Strain Fitness in Pseudomonas putida KT2440 around PP_0817

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_0816 and PP_0817 are separated by 312 nucleotidesPP_0817 and PP_0818 are separated by 113 nucleotides PP_0816: PP_0816 - protoheme IX farnesyltransferase, at 954,159 to 955,046 _0816 PP_0817: PP_0817 - aminotransferase, at 955,359 to 956,567 _0817 PP_0818: PP_0818 - conserved protein of unknown function, at 956,681 to 957,808 _0818 Position (kb) 955 956 957Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 955.035 kb on + strandat 955.036 kb on - strandat 955.036 kb on - strandat 955.036 kb on - strandat 955.036 kb on - strandat 955.145 kb on + strandat 955.145 kb on + strandat 955.145 kb on + strandat 955.159 kb on - strandat 955.159 kb on - strandat 955.340 kb on + strandat 955.340 kb on + strandat 955.340 kb on + strandat 955.341 kb on - strandat 955.414 kb on - strandat 955.534 kb on + strand, within PP_0817at 955.535 kb on - strand, within PP_0817at 955.535 kb on - strand, within PP_0817at 955.618 kb on - strand, within PP_0817at 955.618 kb on - strand, within PP_0817at 955.630 kb on - strand, within PP_0817at 955.727 kb on + strand, within PP_0817at 955.768 kb on - strand, within PP_0817at 955.965 kb on + strand, within PP_0817at 955.965 kb on + strand, within PP_0817at 955.966 kb on - strand, within PP_0817at 955.966 kb on - strand, within PP_0817at 955.966 kb on - strand, within PP_0817at 955.972 kb on + strand, within PP_0817at 955.972 kb on + strand, within PP_0817at 955.973 kb on - strand, within PP_0817at 955.973 kb on - strand, within PP_0817at 955.995 kb on + strand, within PP_0817at 956.094 kb on + strand, within PP_0817at 956.094 kb on + strand, within PP_0817at 956.094 kb on + strand, within PP_0817at 956.172 kb on + strand, within PP_0817at 956.172 kb on + strand, within PP_0817at 956.172 kb on + strand, within PP_0817at 956.343 kb on + strand, within PP_0817at 956.343 kb on + strand, within PP_0817at 956.343 kb on + strand, within PP_0817at 956.343 kb on + strand, within PP_0817at 956.370 kb on + strand, within PP_0817at 956.458 kb on - strandat 956.565 kb on + strandat 956.645 kb on - strandat 956.782 kb on + strandat 956.996 kb on + strand, within PP_0818at 956.997 kb on - strand, within PP_0818at 957.068 kb on + strand, within PP_0818at 957.240 kb on + strand, within PP_0818at 957.252 kb on + strand, within PP_0818at 957.252 kb on + strand, within PP_0818at 957.252 kb on + strand, within PP_0818at 957.252 kb on + strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.253 kb on - strand, within PP_0818at 957.288 kb on + strand, within PP_0818at 957.288 kb on + strand, within PP_0818at 957.289 kb on - strand, within PP_0818at 957.317 kb on - strand, within PP_0818at 957.317 kb on - strand, within PP_0818at 957.330 kb on - strand, within PP_0818at 957.330 kb on - strand, within PP_0818at 957.330 kb on - strand, within PP_0818at 957.333 kb on + strand, within PP_0818at 957.333 kb on + strand, within PP_0818at 957.334 kb on - strand, within PP_0818at 957.334 kb on - strand, within PP_0818at 957.334 kb on - strand, within PP_0818at 957.334 kb on - strand, within PP_0818at 957.341 kb on + strand, within PP_0818at 957.342 kb on - strand, within PP_0818at 957.409 kb on - strand, within PP_0818at 957.409 kb on - strand, within PP_0818

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Per-strain Table

Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
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955,035 + +0.1
955,036 - -1.6
955,036 - -1.1
955,036 - +2.3
955,036 - +0.3
955,145 + -0.4
955,145 + +0.1
955,145 + -0.0
955,159 - -1.4
955,159 - -0.0
955,340 + +0.2
955,340 + +3.9
955,340 + +0.7
955,341 - -0.4
955,414 - +0.8
955,534 + PP_0817 0.14 +0.9
955,535 - PP_0817 0.15 +0.2
955,535 - PP_0817 0.15 -0.8
955,618 - PP_0817 0.21 -0.6
955,618 - PP_0817 0.21 -0.2
955,630 - PP_0817 0.22 +1.6
955,727 + PP_0817 0.30 -0.8
955,768 - PP_0817 0.34 -1.3
955,965 + PP_0817 0.50 -0.6
955,965 + PP_0817 0.50 -0.4
955,966 - PP_0817 0.50 +2.9
955,966 - PP_0817 0.50 -0.2
955,966 - PP_0817 0.50 -0.4
955,972 + PP_0817 0.51 -0.2
955,972 + PP_0817 0.51 -0.4
955,973 - PP_0817 0.51 -0.7
955,973 - PP_0817 0.51 -2.3
955,995 + PP_0817 0.53 -1.1
956,094 + PP_0817 0.61 +0.3
956,094 + PP_0817 0.61 +0.9
956,094 + PP_0817 0.61 -0.4
956,172 + PP_0817 0.67 +1.8
956,172 + PP_0817 0.67 +0.1
956,172 + PP_0817 0.67 -0.7
956,343 + PP_0817 0.81 -0.1
956,343 + PP_0817 0.81 +1.3
956,343 + PP_0817 0.81 +0.9
956,343 + PP_0817 0.81 -0.5
956,370 + PP_0817 0.84 +0.2
956,458 - -0.7
956,565 + -0.5
956,645 - +0.1
956,782 + +0.4
956,996 + PP_0818 0.28 -0.1
956,997 - PP_0818 0.28 -0.2
957,068 + PP_0818 0.34 +0.1
957,240 + PP_0818 0.50 -0.5
957,252 + PP_0818 0.51 +0.1
957,252 + PP_0818 0.51 +0.3
957,252 + PP_0818 0.51 -0.2
957,252 + PP_0818 0.51 -0.1
957,253 - PP_0818 0.51 +1.0
957,253 - PP_0818 0.51 -0.3
957,253 - PP_0818 0.51 +1.6
957,253 - PP_0818 0.51 -0.2
957,253 - PP_0818 0.51 +1.9
957,253 - PP_0818 0.51 -1.7
957,253 - PP_0818 0.51 +0.7
957,288 + PP_0818 0.54 -0.0
957,288 + PP_0818 0.54 +0.1
957,289 - PP_0818 0.54 +0.2
957,317 - PP_0818 0.56 -1.2
957,317 - PP_0818 0.56 -0.4
957,330 - PP_0818 0.58 -0.5
957,330 - PP_0818 0.58 -1.1
957,330 - PP_0818 0.58 +1.3
957,333 + PP_0818 0.58 -1.2
957,333 + PP_0818 0.58 -0.6
957,334 - PP_0818 0.58 -0.1
957,334 - PP_0818 0.58 +0.0
957,334 - PP_0818 0.58 -0.9
957,334 - PP_0818 0.58 +0.6
957,341 + PP_0818 0.59 -0.9
957,342 - PP_0818 0.59 -0.8
957,409 - PP_0818 0.65 +0.4
957,409 - PP_0818 0.65 +0.1

Or see this region's nucleotide sequence