Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0784 and PP_0785 are separated by 44 nucleotides PP_0785 and PP_0786 are separated by 330 nucleotides
PP_0784: PP_0784 - conserved protein of unknown function, at 899,891 to 900,508
_0784
PP_0785: PP_0785 - putative cysZ protein, required for sulfate transport, at 900,553 to 901,281
_0785
PP_0786: PP_0786 - thioredoxin reductase, at 901,612 to 902,574
_0786
Position (kb)
900
901
902 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 899.600 kb on + strand at 899.601 kb on - strand at 899.778 kb on - strand at 899.781 kb on + strand at 899.781 kb on + strand at 899.871 kb on - strand at 899.916 kb on + strand at 900.008 kb on + strand, within PP_0784 at 900.036 kb on - strand, within PP_0784 at 900.128 kb on - strand, within PP_0784 at 900.131 kb on + strand, within PP_0784 at 900.215 kb on - strand, within PP_0784 at 900.323 kb on - strand, within PP_0784 at 900.357 kb on + strand, within PP_0784 at 900.358 kb on - strand, within PP_0784 at 900.453 kb on + strand at 900.453 kb on + strand at 900.453 kb on + strand at 900.453 kb on + strand at 900.454 kb on - strand at 900.454 kb on - strand at 900.454 kb on - strand at 900.454 kb on - strand at 900.456 kb on + strand at 900.488 kb on + strand at 900.489 kb on - strand at 900.507 kb on - strand at 900.582 kb on + strand at 900.583 kb on - strand at 900.583 kb on - strand at 900.644 kb on + strand, within PP_0785 at 900.746 kb on + strand, within PP_0785 at 900.747 kb on - strand, within PP_0785 at 900.896 kb on - strand, within PP_0785 at 900.952 kb on + strand, within PP_0785 at 900.952 kb on + strand, within PP_0785 at 900.952 kb on + strand, within PP_0785 at 900.952 kb on + strand, within PP_0785 at 900.953 kb on - strand, within PP_0785 at 900.953 kb on - strand, within PP_0785 at 901.002 kb on + strand, within PP_0785 at 901.002 kb on + strand, within PP_0785 at 901.002 kb on + strand, within PP_0785 at 901.073 kb on + strand, within PP_0785 at 901.073 kb on + strand, within PP_0785 at 901.074 kb on - strand, within PP_0785 at 901.074 kb on - strand, within PP_0785 at 901.074 kb on - strand, within PP_0785 at 901.086 kb on + strand, within PP_0785 at 901.087 kb on - strand, within PP_0785 at 901.087 kb on - strand, within PP_0785 at 901.151 kb on + strand, within PP_0785 at 901.151 kb on + strand, within PP_0785 at 901.250 kb on + strand at 901.251 kb on - strand at 901.251 kb on - strand at 901.283 kb on + strand at 901.283 kb on + strand at 901.283 kb on + strand at 901.284 kb on - strand at 901.284 kb on - strand at 901.318 kb on + strand at 901.370 kb on + strand at 901.370 kb on + strand at 901.371 kb on - strand at 901.371 kb on - strand at 901.371 kb on - strand at 901.395 kb on - strand at 901.395 kb on - strand at 901.413 kb on + strand at 901.424 kb on + strand at 901.425 kb on - strand at 901.425 kb on - strand at 901.425 kb on - strand at 901.425 kb on - strand at 901.425 kb on - strand at 901.425 kb on - strand at 901.440 kb on + strand at 901.454 kb on + strand at 901.454 kb on + strand at 901.513 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 899,600 + +0.1 899,601 - -1.0 899,778 - +0.9 899,781 + -0.1 899,781 + -1.6 899,871 - -2.1 899,916 + +1.9 900,008 + PP_0784 0.19 +0.2 900,036 - PP_0784 0.23 +1.6 900,128 - PP_0784 0.38 +1.7 900,131 + PP_0784 0.39 +0.2 900,215 - PP_0784 0.52 -1.4 900,323 - PP_0784 0.70 -1.8 900,357 + PP_0784 0.75 -0.3 900,358 - PP_0784 0.76 -0.6 900,453 + +0.2 900,453 + -2.0 900,453 + +0.8 900,453 + -0.1 900,454 - +2.4 900,454 - -0.6 900,454 - +1.3 900,454 - +0.1 900,456 + -0.9 900,488 + +0.9 900,489 - -0.1 900,507 - -1.4 900,582 + -0.6 900,583 - -0.5 900,583 - +0.8 900,644 + PP_0785 0.12 -1.0 900,746 + PP_0785 0.26 -2.3 900,747 - PP_0785 0.27 -1.2 900,896 - PP_0785 0.47 -1.1 900,952 + PP_0785 0.55 +0.1 900,952 + PP_0785 0.55 +0.1 900,952 + PP_0785 0.55 -1.7 900,952 + PP_0785 0.55 -1.1 900,953 - PP_0785 0.55 +1.4 900,953 - PP_0785 0.55 -1.3 901,002 + PP_0785 0.62 -1.2 901,002 + PP_0785 0.62 -0.3 901,002 + PP_0785 0.62 +1.6 901,073 + PP_0785 0.71 +0.3 901,073 + PP_0785 0.71 +2.9 901,074 - PP_0785 0.71 -1.1 901,074 - PP_0785 0.71 +1.4 901,074 - PP_0785 0.71 -1.5 901,086 + PP_0785 0.73 +0.0 901,087 - PP_0785 0.73 -1.0 901,087 - PP_0785 0.73 +1.9 901,151 + PP_0785 0.82 +0.7 901,151 + PP_0785 0.82 +1.1 901,250 + -0.3 901,251 - -0.1 901,251 - -1.5 901,283 + +0.1 901,283 + -0.1 901,283 + +1.9 901,284 - -0.3 901,284 - -1.4 901,318 + -1.0 901,370 + -2.2 901,370 + -0.6 901,371 - -0.2 901,371 - -1.0 901,371 - -1.4 901,395 - +0.1 901,395 - +1.2 901,413 + +1.1 901,424 + -0.1 901,425 - +3.9 901,425 - -0.6 901,425 - +0.7 901,425 - +0.2 901,425 - +1.6 901,425 - +1.2 901,440 + +2.4 901,454 + -0.5 901,454 + +0.1 901,513 + -2.4
Or see this region's nucleotide sequence