Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0698 and PP_0699 are separated by 227 nucleotides PP_0699 and PP_t70 are separated by 130 nucleotides PP_t70 and PP_0700 are separated by 95 nucleotides
PP_0698: PP_0698 - Transcriptional regulator, LysR family, at 811,334 to 812,251
_0698
PP_0699: PP_0699 - Transporter, LysE family, at 812,479 to 813,093
_0699
PP_t70: PP_t70 - tRNA-Met, at 813,224 to 813,300
_t70
PP_0700: PP_0700 - putative Transmembrane sensor, at 813,396 to 814,382
_0700
Position (kb) 812
813
814 Strain fitness (log2 ratio) -2
-1
0
1
2 at 811.485 kb on + strand, within PP_0698 at 811.611 kb on + strand, within PP_0698 at 811.612 kb on - strand, within PP_0698 at 811.788 kb on + strand, within PP_0698 at 811.788 kb on + strand, within PP_0698 at 811.788 kb on + strand, within PP_0698 at 811.789 kb on - strand, within PP_0698 at 811.789 kb on - strand, within PP_0698 at 811.789 kb on - strand at 812.135 kb on + strand, within PP_0698 at 812.136 kb on - strand, within PP_0698 at 812.136 kb on - strand, within PP_0698 at 812.136 kb on - strand, within PP_0698 at 812.136 kb on - strand, within PP_0698 at 812.136 kb on - strand, within PP_0698 at 812.301 kb on - strand at 812.301 kb on - strand at 812.301 kb on - strand at 812.312 kb on + strand at 812.312 kb on + strand at 812.312 kb on + strand at 812.312 kb on + strand at 812.313 kb on - strand at 812.313 kb on - strand at 812.313 kb on - strand at 812.313 kb on - strand at 812.323 kb on - strand at 812.361 kb on + strand at 812.362 kb on - strand at 812.362 kb on - strand at 812.362 kb on - strand at 812.366 kb on - strand at 812.401 kb on - strand at 812.422 kb on + strand at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.638 kb on + strand, within PP_0699 at 812.639 kb on - strand at 812.639 kb on - strand, within PP_0699 at 812.734 kb on + strand, within PP_0699 at 812.735 kb on - strand, within PP_0699 at 812.735 kb on - strand, within PP_0699 at 812.760 kb on + strand, within PP_0699 at 812.760 kb on + strand, within PP_0699 at 812.798 kb on + strand, within PP_0699 at 812.986 kb on - strand, within PP_0699 at 813.086 kb on + strand at 813.086 kb on + strand at 813.086 kb on + strand at 813.086 kb on + strand at 813.087 kb on - strand at 813.103 kb on - strand at 813.504 kb on + strand, within PP_0700 at 813.505 kb on - strand, within PP_0700 at 813.650 kb on + strand, within PP_0700 at 813.651 kb on - strand, within PP_0700 at 813.651 kb on - strand, within PP_0700 at 813.783 kb on + strand, within PP_0700 at 813.784 kb on - strand, within PP_0700 at 813.802 kb on + strand, within PP_0700 at 813.803 kb on - strand, within PP_0700 at 813.803 kb on - strand, within PP_0700 at 813.804 kb on + strand, within PP_0700 at 813.805 kb on - strand, within PP_0700 at 813.805 kb on - strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.972 kb on + strand, within PP_0700 at 813.973 kb on - strand, within PP_0700 at 814.037 kb on - strand, within PP_0700 at 814.037 kb on - strand, within PP_0700 at 814.042 kb on + strand, within PP_0700 at 814.043 kb on - strand, within PP_0700 at 814.043 kb on - strand, within PP_0700
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 811,485 + PP_0698 0.16 +1.0 811,611 + PP_0698 0.30 +1.0 811,612 - PP_0698 0.30 +0.0 811,788 + PP_0698 0.49 -2.0 811,788 + PP_0698 0.49 -1.7 811,788 + PP_0698 0.49 -0.8 811,789 - PP_0698 0.50 -0.3 811,789 - PP_0698 0.50 -0.5 811,789 - +2.4 812,135 + PP_0698 0.87 -0.4 812,136 - PP_0698 0.87 -0.8 812,136 - PP_0698 0.87 -0.9 812,136 - PP_0698 0.87 +1.1 812,136 - PP_0698 0.87 +0.0 812,136 - PP_0698 0.87 -0.2 812,301 - -1.3 812,301 - -0.9 812,301 - -0.2 812,312 + +0.6 812,312 + +0.8 812,312 + -0.2 812,312 + -2.0 812,313 - +0.5 812,313 - +0.8 812,313 - -0.9 812,313 - -0.6 812,323 - +0.4 812,361 + +0.1 812,362 - -0.2 812,362 - +1.0 812,362 - -0.3 812,366 - -1.0 812,401 - -0.6 812,422 + -0.9 812,638 + PP_0699 0.26 +0.1 812,638 + PP_0699 0.26 -0.4 812,638 + PP_0699 0.26 -0.3 812,638 + PP_0699 0.26 -0.7 812,639 - +1.0 812,639 - PP_0699 0.26 -0.8 812,734 + PP_0699 0.41 -0.2 812,735 - PP_0699 0.42 +0.3 812,735 - PP_0699 0.42 -0.7 812,760 + PP_0699 0.46 -0.5 812,760 + PP_0699 0.46 -0.5 812,798 + PP_0699 0.52 -1.0 812,986 - PP_0699 0.82 +0.1 813,086 + -0.8 813,086 + +0.4 813,086 + -0.7 813,086 + -0.7 813,087 - -1.0 813,103 - +0.1 813,504 + PP_0700 0.11 -0.8 813,505 - PP_0700 0.11 -0.3 813,650 + PP_0700 0.26 +0.2 813,651 - PP_0700 0.26 -2.0 813,651 - PP_0700 0.26 +2.0 813,783 + PP_0700 0.39 -2.5 813,784 - PP_0700 0.39 -0.2 813,802 + PP_0700 0.41 -2.1 813,803 - PP_0700 0.41 +0.2 813,803 - PP_0700 0.41 +0.0 813,804 + PP_0700 0.41 -1.3 813,805 - PP_0700 0.41 -1.3 813,805 - PP_0700 0.41 +0.2 813,972 + PP_0700 0.58 -0.4 813,972 + PP_0700 0.58 -0.4 813,972 + PP_0700 0.58 -0.3 813,973 - PP_0700 0.58 -0.8 814,037 - PP_0700 0.65 -1.6 814,037 - PP_0700 0.65 -0.5 814,042 + PP_0700 0.65 -1.6 814,043 - PP_0700 0.66 -1.2 814,043 - PP_0700 0.66 -0.8
Or see this region's nucleotide sequence