Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0521 and PP_0522 are separated by 180 nucleotides PP_0522 and PP_0523 overlap by 4 nucleotides PP_0523 and PP_0524 overlap by 1 nucleotides PP_0524 and PP_0525 overlap by 23 nucleotides
PP_0521: PP_0521 - conserved exported protein of unknown function, at 606,173 to 606,916
_0521
PP_0522: PP_0522 - GTP cyclohydrolase-2, at 607,097 to 607,714
_0522
PP_0523: PP_0523 - conserved membrane protein of unknown function, at 607,711 to 608,130
_0523
PP_0524: PP_0524 - putative Periplasmic cobalamin-binding protein HutB, at 608,130 to 608,942
_0524
PP_0525: PP_0525 - TonB-dependent receptor, B12 family, at 608,920 to 610,845
_0525
Position (kb) 607
608
609 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 606.764 kb on + strand, within PP_0521 at 606.764 kb on + strand, within PP_0521 at 606.765 kb on - strand, within PP_0521 at 606.765 kb on - strand, within PP_0521 at 606.765 kb on - strand, within PP_0521 at 606.782 kb on - strand, within PP_0521 at 606.837 kb on + strand, within PP_0521 at 606.837 kb on + strand, within PP_0521 at 606.837 kb on + strand, within PP_0521 at 606.837 kb on + strand, within PP_0521 at 606.838 kb on - strand, within PP_0521 at 606.841 kb on + strand, within PP_0521 at 606.884 kb on + strand at 606.924 kb on + strand at 606.925 kb on - strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.934 kb on + strand at 606.935 kb on - strand at 606.935 kb on - strand at 606.935 kb on - strand at 606.936 kb on + strand at 606.937 kb on - strand at 606.983 kb on + strand at 606.986 kb on + strand at 606.986 kb on + strand at 606.987 kb on - strand at 607.008 kb on + strand at 607.018 kb on + strand at 607.018 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.020 kb on + strand at 607.021 kb on - strand at 607.051 kb on + strand at 607.080 kb on + strand at 607.080 kb on + strand at 607.900 kb on + strand at 607.900 kb on + strand, within PP_0523 at 607.901 kb on - strand, within PP_0523 at 607.914 kb on + strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.915 kb on - strand, within PP_0523 at 607.939 kb on - strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.997 kb on + strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.998 kb on - strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 607.999 kb on + strand, within PP_0523 at 608.000 kb on - strand, within PP_0523 at 608.000 kb on - strand, within PP_0523 at 608.000 kb on - strand, within PP_0523 at 608.000 kb on - strand, within PP_0523 at 608.090 kb on + strand at 608.091 kb on - strand at 608.091 kb on - strand at 608.091 kb on - strand at 608.091 kb on - strand at 608.091 kb on - strand at 608.098 kb on + strand at 608.099 kb on - strand at 608.099 kb on - strand at 608.104 kb on + strand at 608.140 kb on + strand at 608.140 kb on + strand at 608.141 kb on - strand at 608.141 kb on - strand at 608.141 kb on - strand at 608.141 kb on - strand at 608.173 kb on + strand at 608.174 kb on - strand at 608.174 kb on - strand at 608.191 kb on + strand at 608.191 kb on + strand at 608.191 kb on + strand at 608.192 kb on - strand at 608.529 kb on + strand, within PP_0524 at 608.574 kb on + strand, within PP_0524 at 608.604 kb on + strand, within PP_0524 at 608.629 kb on - strand, within PP_0524 at 608.629 kb on - strand, within PP_0524 at 608.728 kb on + strand, within PP_0524 at 608.728 kb on + strand, within PP_0524 at 608.728 kb on + strand, within PP_0524 at 608.728 kb on + strand, within PP_0524 at 608.748 kb on - strand, within PP_0524 at 608.748 kb on - strand, within PP_0524 at 608.921 kb on + strand at 608.981 kb on + strand at 608.981 kb on + strand at 609.002 kb on + strand at 609.002 kb on + strand at 609.003 kb on - strand at 609.003 kb on - strand at 609.003 kb on - strand at 609.017 kb on + strand at 609.018 kb on - strand at 609.058 kb on - strand at 609.073 kb on + strand at 609.074 kb on - strand at 609.123 kb on + strand, within PP_0525 at 609.123 kb on + strand at 609.124 kb on - strand, within PP_0525 at 609.124 kb on - strand, within PP_0525
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 606,764 + PP_0521 0.79 -1.2 606,764 + PP_0521 0.79 +0.0 606,765 - PP_0521 0.80 -0.5 606,765 - PP_0521 0.80 -1.9 606,765 - PP_0521 0.80 +1.7 606,782 - PP_0521 0.82 -0.5 606,837 + PP_0521 0.89 +0.1 606,837 + PP_0521 0.89 +0.1 606,837 + PP_0521 0.89 +0.1 606,837 + PP_0521 0.89 +0.3 606,838 - PP_0521 0.89 +0.7 606,841 + PP_0521 0.90 -1.3 606,884 + +1.2 606,924 + +1.0 606,925 - +2.0 606,934 + -0.5 606,934 + -0.9 606,934 + +1.2 606,934 + -0.3 606,934 + +0.4 606,934 + -0.6 606,934 + +0.8 606,935 - +0.3 606,935 - -1.0 606,935 - +0.2 606,936 + -0.3 606,937 - +0.0 606,983 + +0.3 606,986 + -0.5 606,986 + -0.4 606,987 - -0.2 607,008 + +1.0 607,018 + -0.6 607,018 + -0.2 607,020 + +0.8 607,020 + -0.0 607,020 + +0.5 607,020 + +0.9 607,020 + -1.0 607,020 + +0.1 607,020 + -0.5 607,021 - +1.4 607,051 + +1.7 607,080 + +1.6 607,080 + -0.1 607,900 + +2.0 607,900 + PP_0523 0.45 -1.8 607,901 - PP_0523 0.45 -0.2 607,914 + PP_0523 0.48 +0.1 607,915 - PP_0523 0.49 -0.1 607,915 - PP_0523 0.49 -1.9 607,915 - PP_0523 0.49 -0.7 607,915 - PP_0523 0.49 -1.0 607,915 - PP_0523 0.49 +1.7 607,915 - PP_0523 0.49 -1.2 607,939 - PP_0523 0.54 +0.4 607,997 + PP_0523 0.68 -1.3 607,997 + PP_0523 0.68 -0.9 607,997 + PP_0523 0.68 +0.7 607,997 + PP_0523 0.68 +1.1 607,997 + PP_0523 0.68 -0.1 607,997 + PP_0523 0.68 -1.7 607,997 + PP_0523 0.68 +1.7 607,997 + PP_0523 0.68 +0.3 607,997 + PP_0523 0.68 -0.6 607,997 + PP_0523 0.68 -0.2 607,997 + PP_0523 0.68 -1.2 607,997 + PP_0523 0.68 +1.2 607,997 + PP_0523 0.68 +0.3 607,998 - PP_0523 0.68 -1.2 607,998 - PP_0523 0.68 -0.1 607,998 - PP_0523 0.68 +0.6 607,998 - PP_0523 0.68 -0.3 607,998 - PP_0523 0.68 -0.7 607,998 - PP_0523 0.68 -0.8 607,998 - PP_0523 0.68 +0.3 607,998 - PP_0523 0.68 -0.7 607,998 - PP_0523 0.68 +2.0 607,998 - PP_0523 0.68 +0.2 607,998 - PP_0523 0.68 -1.2 607,998 - PP_0523 0.68 -0.7 607,998 - PP_0523 0.68 +0.2 607,999 + PP_0523 0.69 +0.7 607,999 + PP_0523 0.69 +1.2 607,999 + PP_0523 0.69 +1.4 607,999 + PP_0523 0.69 -2.4 607,999 + PP_0523 0.69 -1.9 607,999 + PP_0523 0.69 +0.2 607,999 + PP_0523 0.69 -0.6 607,999 + PP_0523 0.69 -0.1 607,999 + PP_0523 0.69 -0.4 607,999 + PP_0523 0.69 -0.3 608,000 - PP_0523 0.69 -1.7 608,000 - PP_0523 0.69 +1.1 608,000 - PP_0523 0.69 -1.3 608,000 - PP_0523 0.69 -0.6 608,090 + +0.7 608,091 - -1.0 608,091 - +0.4 608,091 - +3.0 608,091 - +0.2 608,091 - -0.7 608,098 + +0.2 608,099 - -1.5 608,099 - -0.1 608,104 + +0.4 608,140 + -1.7 608,140 + -2.1 608,141 - -0.2 608,141 - -0.0 608,141 - +1.2 608,141 - -0.8 608,173 + +1.4 608,174 - -0.3 608,174 - -2.6 608,191 + -0.7 608,191 + -1.6 608,191 + +0.4 608,192 - +0.5 608,529 + PP_0524 0.49 -0.7 608,574 + PP_0524 0.55 -0.4 608,604 + PP_0524 0.58 -1.2 608,629 - PP_0524 0.61 -0.4 608,629 - PP_0524 0.61 +0.1 608,728 + PP_0524 0.74 +0.6 608,728 + PP_0524 0.74 -0.2 608,728 + PP_0524 0.74 +1.0 608,728 + PP_0524 0.74 -1.5 608,748 - PP_0524 0.76 +0.6 608,748 - PP_0524 0.76 +1.0 608,921 + +1.7 608,981 + +1.6 608,981 + -0.5 609,002 + -0.2 609,002 + -0.2 609,003 - +0.2 609,003 - +0.4 609,003 - -0.4 609,017 + +0.1 609,018 - -1.7 609,058 - +0.2 609,073 + +0.8 609,074 - +1.2 609,123 + PP_0525 0.11 +1.2 609,123 + +0.1 609,124 - PP_0525 0.11 -1.8 609,124 - PP_0525 0.11 +0.9
Or see this region's nucleotide sequence