Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0404 and PP_0405 are separated by 130 nucleotides PP_0405 and PP_0406 overlap by 4 nucleotides PP_0406 and PP_0407 are separated by 1 nucleotides
PP_0404: PP_0404 - LPS-assembly protein LptD, at 489,598 to 492,420
_0404
PP_0405: PP_0405 - conserved protein of unknown function, at 492,551 to 493,570
_0405
PP_0406: PP_0406 - Nucleotidyltransferase family protein, at 493,567 to 494,238
_0406
PP_0407: PP_0407 - DnaJ-like protein DjlA, at 494,240 to 495,007
_0407
Position (kb) 492
493
494 Strain fitness (log2 ratio) -2
-1
0
1 at 492.506 kb on - strand at 492.567 kb on + strand at 492.567 kb on + strand at 492.567 kb on + strand at 492.567 kb on + strand at 492.568 kb on - strand at 492.568 kb on - strand at 492.568 kb on - strand at 492.568 kb on - strand at 492.693 kb on - strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.804 kb on + strand, within PP_0405 at 492.805 kb on - strand, within PP_0405 at 492.805 kb on - strand, within PP_0405 at 492.805 kb on - strand, within PP_0405 at 492.805 kb on - strand, within PP_0405 at 492.805 kb on - strand, within PP_0405 at 492.878 kb on + strand, within PP_0405 at 492.878 kb on + strand, within PP_0405 at 492.878 kb on + strand, within PP_0405 at 492.878 kb on + strand, within PP_0405 at 492.878 kb on + strand, within PP_0405 at 492.879 kb on - strand, within PP_0405 at 492.879 kb on - strand, within PP_0405 at 492.983 kb on + strand, within PP_0405 at 492.984 kb on - strand, within PP_0405 at 492.984 kb on - strand, within PP_0405 at 493.031 kb on + strand, within PP_0405 at 493.032 kb on - strand, within PP_0405 at 493.032 kb on - strand, within PP_0405 at 493.141 kb on - strand, within PP_0405 at 493.141 kb on - strand at 493.141 kb on - strand, within PP_0405 at 493.141 kb on - strand, within PP_0405 at 493.141 kb on - strand, within PP_0405 at 493.141 kb on - strand, within PP_0405 at 493.151 kb on + strand, within PP_0405 at 493.151 kb on + strand, within PP_0405 at 493.152 kb on - strand, within PP_0405 at 493.152 kb on - strand, within PP_0405 at 493.216 kb on - strand, within PP_0405 at 493.217 kb on + strand, within PP_0405 at 493.217 kb on + strand, within PP_0405 at 493.232 kb on + strand, within PP_0405 at 493.232 kb on + strand, within PP_0405 at 493.233 kb on - strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.240 kb on + strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.241 kb on - strand, within PP_0405 at 493.454 kb on + strand, within PP_0405 at 493.455 kb on - strand, within PP_0405 at 493.484 kb on + strand at 493.485 kb on - strand at 493.485 kb on - strand at 493.486 kb on + strand at 493.487 kb on - strand at 493.487 kb on - strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.488 kb on + strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.489 kb on - strand at 493.673 kb on - strand, within PP_0406 at 493.901 kb on - strand, within PP_0406 at 494.147 kb on + strand, within PP_0406 at 494.148 kb on - strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.161 kb on + strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.162 kb on - strand, within PP_0406 at 494.473 kb on + strand, within PP_0407 at 494.473 kb on + strand, within PP_0407
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 492,506 - -0.9 492,567 + -1.8 492,567 + -1.1 492,567 + -1.6 492,567 + -1.2 492,568 - +0.1 492,568 - -0.6 492,568 - -0.4 492,568 - +1.2 492,693 - PP_0405 0.14 +0.1 492,804 + PP_0405 0.25 -0.4 492,804 + PP_0405 0.25 -0.5 492,804 + PP_0405 0.25 -0.8 492,804 + PP_0405 0.25 -0.0 492,804 + PP_0405 0.25 -0.6 492,804 + PP_0405 0.25 -0.0 492,804 + PP_0405 0.25 -0.5 492,804 + PP_0405 0.25 -0.4 492,804 + PP_0405 0.25 -0.6 492,804 + PP_0405 0.25 -0.3 492,804 + PP_0405 0.25 -0.5 492,805 - PP_0405 0.25 +0.2 492,805 - PP_0405 0.25 -0.8 492,805 - PP_0405 0.25 -1.0 492,805 - PP_0405 0.25 +0.2 492,805 - PP_0405 0.25 -1.3 492,878 + PP_0405 0.32 -0.6 492,878 + PP_0405 0.32 -0.6 492,878 + PP_0405 0.32 -0.3 492,878 + PP_0405 0.32 -0.9 492,878 + PP_0405 0.32 -1.1 492,879 - PP_0405 0.32 -0.5 492,879 - PP_0405 0.32 -0.5 492,983 + PP_0405 0.42 -0.4 492,984 - PP_0405 0.42 -0.3 492,984 - PP_0405 0.42 -0.4 493,031 + PP_0405 0.47 -0.5 493,032 - PP_0405 0.47 -0.0 493,032 - PP_0405 0.47 -0.7 493,141 - PP_0405 0.58 -0.8 493,141 - +0.8 493,141 - PP_0405 0.58 +0.2 493,141 - PP_0405 0.58 -1.2 493,141 - PP_0405 0.58 -0.3 493,141 - PP_0405 0.58 -0.6 493,151 + PP_0405 0.59 -1.4 493,151 + PP_0405 0.59 -1.1 493,152 - PP_0405 0.59 -0.2 493,152 - PP_0405 0.59 -0.9 493,216 - PP_0405 0.65 -0.3 493,217 + PP_0405 0.65 -1.7 493,217 + PP_0405 0.65 -0.6 493,232 + PP_0405 0.67 -0.7 493,232 + PP_0405 0.67 +0.0 493,233 - PP_0405 0.67 -0.5 493,240 + PP_0405 0.68 -1.2 493,240 + PP_0405 0.68 +0.0 493,240 + PP_0405 0.68 -1.1 493,240 + PP_0405 0.68 -1.0 493,240 + PP_0405 0.68 -0.3 493,240 + PP_0405 0.68 -0.1 493,241 - PP_0405 0.68 -0.4 493,241 - PP_0405 0.68 -1.3 493,454 + PP_0405 0.89 -1.4 493,455 - PP_0405 0.89 -0.4 493,484 + -0.7 493,485 - -1.0 493,485 - -1.9 493,486 + -0.9 493,487 - -0.3 493,487 - -0.3 493,488 + -0.1 493,488 + -0.4 493,488 + -1.0 493,489 - -1.1 493,489 - -0.3 493,489 - -0.3 493,489 - -1.3 493,489 - -0.5 493,489 - -0.9 493,673 - PP_0406 0.16 -0.9 493,901 - PP_0406 0.50 -0.3 494,147 + PP_0406 0.86 -0.9 494,148 - PP_0406 0.86 -0.8 494,161 + PP_0406 0.88 -2.3 494,161 + PP_0406 0.88 -0.0 494,162 - PP_0406 0.89 -1.6 494,162 - PP_0406 0.89 -2.6 494,162 - PP_0406 0.89 -2.5 494,473 + PP_0407 0.30 -0.2 494,473 + PP_0407 0.30 -0.3
Or see this region's nucleotide sequence