Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PP_0351 and PP_0352 overlap by 4 nucleotides PP_0352 and PP_0353 are separated by 147 nucleotides PP_0353 and PP_0354 overlap by 4 nucleotides
PP_0351: PP_0351 - putative Transmembrane sensor protein, at 428,050 to 429,006
_0351
PP_0352: PP_0352 - RNA polymerase sigma-70 factor, ECF subfamily, at 429,003 to 429,521
_0352
PP_0353: PP_0353 - Exonuclease, at 429,669 to 430,379
_0353
PP_0354: PP_0354 - CBS domain protein, at 430,376 to 432,313
_0354
Position (kb) 429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 428.051 kb on + strand at 428.051 kb on + strand at 428.082 kb on + strand at 428.082 kb on + strand at 428.083 kb on - strand at 428.083 kb on - strand at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.248 kb on + strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.249 kb on - strand, within PP_0351 at 428.425 kb on - strand, within PP_0351 at 428.492 kb on - strand, within PP_0351 at 428.577 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.645 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351 at 428.682 kb on - strand, within PP_0351 at 429.031 kb on + strand at 429.031 kb on + strand at 429.031 kb on + strand at 429.032 kb on - strand at 429.032 kb on - strand at 429.032 kb on - strand at 429.058 kb on + strand, within PP_0352 at 429.059 kb on - strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.268 kb on + strand, within PP_0352 at 429.476 kb on - strand at 429.641 kb on + strand at 429.642 kb on - strand at 429.664 kb on + strand at 429.665 kb on - strand at 429.670 kb on + strand at 429.670 kb on + strand at 429.671 kb on - strand at 430.104 kb on + strand, within PP_0353 at 430.104 kb on + strand, within PP_0353 at 430.105 kb on - strand, within PP_0353 at 430.105 kb on - strand, within PP_0353 at 430.105 kb on - strand, within PP_0353 at 430.206 kb on + strand, within PP_0353 at 430.207 kb on - strand, within PP_0353 at 430.207 kb on - strand, within PP_0353 at 430.277 kb on + strand, within PP_0353 at 430.277 kb on + strand, within PP_0353 at 430.277 kb on + strand, within PP_0353 at 430.277 kb on + strand, within PP_0353 at 430.277 kb on + strand, within PP_0353 at 430.278 kb on - strand, within PP_0353 at 430.278 kb on - strand, within PP_0353 at 430.281 kb on + strand, within PP_0353 at 430.282 kb on - strand, within PP_0353 at 430.282 kb on - strand, within PP_0353 at 430.379 kb on + strand at 430.379 kb on + strand at 430.379 kb on + strand at 430.380 kb on - strand at 430.380 kb on - strand at 430.380 kb on - strand at 430.380 kb on - strand at 430.491 kb on + strand at 430.491 kb on + strand at 430.508 kb on + strand at 430.509 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO. remove 428,051 + +0.1 428,051 + -0.3 428,082 + +0.1 428,082 + -1.4 428,083 - -1.1 428,083 - -1.5 428,248 + PP_0351 0.21 -1.0 428,248 + PP_0351 0.21 -1.3 428,248 + PP_0351 0.21 -2.2 428,249 - PP_0351 0.21 -2.8 428,249 - PP_0351 0.21 -0.4 428,249 - PP_0351 0.21 +1.0 428,425 - PP_0351 0.39 -1.7 428,492 - PP_0351 0.46 -2.2 428,577 - PP_0351 0.55 +1.2 428,645 - PP_0351 0.62 -1.9 428,645 - PP_0351 0.62 -2.4 428,645 - PP_0351 0.62 -2.4 428,682 - PP_0351 0.66 -2.9 428,682 - PP_0351 0.66 -0.3 429,031 + +1.4 429,031 + +1.2 429,031 + +0.0 429,032 - -0.4 429,032 - +1.1 429,032 - -0.7 429,058 + PP_0352 0.11 -0.4 429,059 - PP_0352 0.11 -1.1 429,268 + PP_0352 0.51 -1.1 429,268 + PP_0352 0.51 -0.9 429,268 + PP_0352 0.51 +0.5 429,476 - +1.1 429,641 + +1.2 429,642 - -0.1 429,664 + -0.8 429,665 - +0.4 429,670 + +1.4 429,670 + -0.8 429,671 - +0.5 430,104 + PP_0353 0.61 -0.1 430,104 + PP_0353 0.61 -0.3 430,105 - PP_0353 0.61 +0.5 430,105 - PP_0353 0.61 -0.1 430,105 - PP_0353 0.61 -1.1 430,206 + PP_0353 0.76 -0.3 430,207 - PP_0353 0.76 -0.5 430,207 - PP_0353 0.76 +0.7 430,277 + PP_0353 0.86 +1.7 430,277 + PP_0353 0.86 -0.9 430,277 + PP_0353 0.86 -0.1 430,277 + PP_0353 0.86 -0.3 430,277 + PP_0353 0.86 +2.0 430,278 - PP_0353 0.86 +0.8 430,278 - PP_0353 0.86 +2.0 430,281 + PP_0353 0.86 -0.5 430,282 - PP_0353 0.86 +1.2 430,282 - PP_0353 0.86 +0.5 430,379 + +0.0 430,379 + +0.8 430,379 + -0.2 430,380 - -0.4 430,380 - +0.2 430,380 - -1.5 430,380 - -0.0 430,491 + +0.8 430,491 + -0.0 430,508 + +3.0 430,509 - -1.9
Or see this region's nucleotide sequence