Strain Fitness in Pseudomonas putida KT2440 around PP_0349

Experiment: 24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	24hr timepoint from 1L M9/1% dextrose BATCH fermentation in 2L Sartorius Biostat reactor kept at 10% DO.
423,200	+		PP_0348	0.73	+0.8
423,200	+		PP_0348	0.73	+0.8
423,200	+		PP_0348	0.73	-0.4
423,200	+		PP_0348	0.73	-0.0
423,200	+		PP_0348	0.73	+0.1
423,201	-		PP_0348	0.73	+0.5
423,201	-		PP_0348	0.73	-1.9
423,201	-		PP_0348	0.73	-0.4
423,204	+		PP_0348	0.73	+0.0
423,205	-		PP_0348	0.73	-0.8
423,205	-		PP_0348	0.73	+0.8
423,276	+		PP_0348	0.76	+1.2
423,277	-		PP_0348	0.76	+0.0
423,277	-		PP_0348	0.76	-0.3
423,277	-		PP_0348	0.76	-1.9
423,325	+		PP_0348	0.78	+0.2
423,326	-		PP_0348	0.78	+0.6
423,326	-		PP_0348	0.78	-1.2
423,373	+		PP_0348	0.79	+0.8
423,373	+		PP_0348	0.79	+1.7
423,568	+		PP_0348	0.86	-0.3
423,569	-		PP_0348	0.86	+0.0
423,638	+		PP_0348	0.89	+0.2
423,638	+		PP_0348	0.89	-0.1
423,638	+		PP_0348	0.89	-1.6
423,638	+		PP_0348	0.89	+0.5
423,639	-		PP_0348	0.89	+0.1
423,639	-		PP_0348	0.89	-0.3
423,639	-		PP_0348	0.89	+0.1
423,639	-		PP_0348	0.89	+0.8
423,639	-		PP_0348	0.89	+0.8
423,681	+				-1.3
423,681	+				+0.5
423,681	+				+2.7
423,681	+				-0.8
423,682	-				-0.6
423,988	+				-0.3
423,989	-				+0.7
424,244	-				-0.7
424,244	-				+0.3
424,319	-		PP_0349	0.11	+1.3
424,363	+		PP_0349	0.15	+0.1
424,416	+		PP_0349	0.20	-1.4
424,417	-		PP_0349	0.20	+1.5
424,472	-		PP_0349	0.25	-1.2
424,472	-		PP_0349	0.25	-2.3
424,472	-		PP_0349	0.25	-1.1
424,674	+		PP_0349	0.43	-0.5
424,774	-		PP_0349	0.52	-0.2
424,810	+		PP_0349	0.55	-0.6
424,810	+		PP_0349	0.55	+0.1
424,811	-		PP_0349	0.55	-0.5
424,848	+		PP_0349	0.58	+0.1
424,848	+		PP_0349	0.58	-0.6
424,849	-		PP_0349	0.59	-1.0
424,849	-		PP_0349	0.59	+0.8
425,179	-		PP_0349	0.88	-0.8
425,207	+				+0.4
425,208	-				+0.2
425,208	-				+0.8
425,208	-				-0.5
425,249	+				-0.6
425,249	+				-0.0
425,250	-				+0.2
425,376	+				-0.8
425,376	+				-1.2
425,376	+				-0.9
425,377	-				-0.3
425,377	-				-0.5
425,377	-				-0.5
425,377	-				-0.8
425,412	+				+1.3
425,412	+				-0.4
425,412	+				-0.0
425,415	+				-0.9
425,416	-				+0.1
425,416	-				-0.5
425,416	-				+0.0
425,421	+				-0.5
425,421	+				+2.4
425,421	+				+0.8
425,422	-				+0.8
425,422	-				-0.5
425,422	-				+0.8
425,422	-				+1.3
425,424	+				-0.1
425,424	+				-1.5
425,424	+				-0.0
425,424	+				-2.6
425,425	-				+1.0
425,425	-				-0.8
425,438	-				+0.1
425,438	-				-1.7
425,438	-				+1.8
425,445	+				-0.4
425,446	-				+0.2
425,446	-				-0.9
425,523	+				+0.7
425,550	+				-0.2
425,571	+				+0.8
425,572	-				-0.6
425,572	-				+1.1
425,575	+				-0.6
425,576	-				-0.5
425,576	-				+0.4
425,576	-				-0.0
425,576	-				+0.7
425,576	-				+0.1
425,596	+				-0.8
425,596	+				-0.3
425,596	+				+0.4
425,597	-				+0.8
425,597	-				-0.5
425,605	+				+0.4
425,661	+		PP_0350	0.12	+2.2
425,661	+		PP_0350	0.12	+0.9
425,661	+		PP_0350	0.12	-0.3
425,662	-		PP_0350	0.12	-2.5
425,754	+		PP_0350	0.16	+0.2
425,941	-		PP_0350	0.24	-0.8
426,037	-		PP_0350	0.28	+0.5
426,073	-		PP_0350	0.29	-0.0
426,115	+		PP_0350	0.31	-0.1
426,197	-		PP_0350	0.34	+1.2
426,197	-		PP_0350	0.34	+0.2
426,222	+		PP_0350	0.35	-0.1
426,222	+		PP_0350	0.35	+0.1
426,222	+		PP_0350	0.35	-0.3
426,223	-		PP_0350	0.35	+0.3
426,223	-		PP_0350	0.35	-2.0
426,223	-		PP_0350	0.35	-1.2
426,252	+		PP_0350	0.36	+1.0
426,258	+		PP_0350	0.37	-1.6