Strain Fitness in Escherichia coli BW25113 around b2592

Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrrsG and clpB are separated by 442 nucleotidesclpB and ryfD overlap by 21 nucleotidesryfD and yfiH are separated by 7 nucleotidesyfiH and rluD overlap by 4 nucleotides b2591: rrsG - 16S ribosomal RNA (NCBI), at 2,727,638 to 2,729,179 rrsG b2592: clpB - protein disaggregation chaperone (NCBI), at 2,729,622 to 2,732,195 clpB b4609: ryfD - Novel sRNA, function unknown (RefSeq), at 2,732,175 to 2,732,317 ryfD b2593: yfiH - hypothetical protein (NCBI), at 2,732,325 to 2,733,056 yfiH b2594: rluD - 23S rRNA pseudouridine synthase (NCBI), at 2,733,053 to 2,734,033 rluD Position (kb) 2729 2730 2731 2732 2733Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 2729.412 kb on - strandat 2729.705 kb on - strandat 2729.753 kb on + strandat 2729.753 kb on + strandat 2729.761 kb on - strandat 2729.900 kb on + strand, within clpBat 2730.084 kb on + strand, within clpBat 2730.092 kb on - strand, within clpBat 2730.173 kb on + strand, within clpBat 2730.189 kb on - strand, within clpBat 2730.269 kb on - strand, within clpBat 2730.269 kb on - strand, within clpBat 2730.304 kb on - strand, within clpBat 2730.304 kb on - strand, within clpBat 2730.346 kb on + strand, within clpBat 2730.357 kb on - strand, within clpBat 2730.357 kb on - strand, within clpBat 2730.392 kb on + strand, within clpBat 2730.456 kb on - strand, within clpBat 2730.516 kb on - strand, within clpBat 2730.565 kb on + strand, within clpBat 2730.755 kb on + strand, within clpBat 2730.787 kb on - strand, within clpBat 2730.798 kb on + strand, within clpBat 2730.798 kb on + strand, within clpBat 2730.862 kb on - strand, within clpBat 2730.901 kb on - strand, within clpBat 2730.901 kb on - strand, within clpBat 2730.902 kb on + strand, within clpBat 2731.039 kb on + strand, within clpBat 2731.048 kb on - strand, within clpBat 2731.121 kb on - strand, within clpBat 2731.121 kb on - strand, within clpBat 2731.133 kb on + strand, within clpBat 2731.156 kb on - strand, within clpBat 2731.267 kb on - strand, within clpBat 2731.267 kb on - strand, within clpBat 2731.351 kb on + strand, within clpBat 2731.448 kb on - strand, within clpBat 2731.468 kb on + strand, within clpBat 2731.476 kb on - strand, within clpBat 2731.476 kb on - strand, within clpBat 2731.481 kb on - strand, within clpBat 2731.531 kb on - strand, within clpBat 2731.575 kb on - strand, within clpBat 2731.674 kb on - strand, within clpBat 2731.730 kb on + strand, within clpBat 2731.736 kb on - strand, within clpBat 2731.746 kb on - strand, within clpBat 2731.746 kb on - strand, within clpBat 2731.857 kb on - strand, within clpBat 2731.868 kb on + strand, within clpBat 2731.919 kb on + strand, within clpBat 2731.927 kb on - strand, within clpBat 2731.965 kb on - strandat 2731.966 kb on - strandat 2731.966 kb on - strandat 2732.002 kb on - strandat 2732.140 kb on - strandat 2732.202 kb on + strand, within ryfDat 2732.205 kb on - strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.212 kb on + strand, within ryfDat 2732.251 kb on + strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.277 kb on - strand, within ryfDat 2732.323 kb on + strandat 2732.323 kb on + strandat 2732.456 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.499 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.502 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.563 kb on - strand, within yfiHat 2732.594 kb on + strand, within yfiHat 2732.602 kb on - strand, within yfiHat 2732.648 kb on + strand, within yfiHat 2732.659 kb on + strand, within yfiHat 2732.669 kb on + strand, within yfiHat 2732.748 kb on + strand, within yfiHat 2732.756 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.763 kb on - strand, within yfiHat 2732.778 kb on + strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.813 kb on - strand, within yfiHat 2732.852 kb on - strand, within yfiHat 2732.854 kb on - strand, within yfiHat 2732.926 kb on + strand, within yfiHat 2732.943 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.969 kb on - strand, within yfiHat 2732.994 kb on + strandat 2733.052 kb on + strandat 2733.060 kb on - strandat 2733.062 kb on - strandat 2733.072 kb on + strandat 2733.079 kb on - strandat 2733.079 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
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2,729,412 - +1.6
2,729,705 - +0.8
2,729,753 + -1.3
2,729,753 + +0.2
2,729,761 - -0.6
2,729,900 + clpB b2592 0.11 +0.4
2,730,084 + clpB b2592 0.18 -0.7
2,730,092 - clpB b2592 0.18 -1.1
2,730,173 + clpB b2592 0.21 -0.2
2,730,189 - clpB b2592 0.22 +1.6
2,730,269 - clpB b2592 0.25 +1.7
2,730,269 - clpB b2592 0.25 -0.2
2,730,304 - clpB b2592 0.26 +0.3
2,730,304 - clpB b2592 0.26 +0.7
2,730,346 + clpB b2592 0.28 -1.2
2,730,357 - clpB b2592 0.29 +0.2
2,730,357 - clpB b2592 0.29 -2.5
2,730,392 + clpB b2592 0.30 -0.5
2,730,456 - clpB b2592 0.32 -0.3
2,730,516 - clpB b2592 0.35 -2.0
2,730,565 + clpB b2592 0.37 +0.8
2,730,755 + clpB b2592 0.44 -0.3
2,730,787 - clpB b2592 0.45 +1.9
2,730,798 + clpB b2592 0.46 +1.8
2,730,798 + clpB b2592 0.46 +0.0
2,730,862 - clpB b2592 0.48 -0.7
2,730,901 - clpB b2592 0.50 -0.2
2,730,901 - clpB b2592 0.50 +0.6
2,730,902 + clpB b2592 0.50 +0.7
2,731,039 + clpB b2592 0.55 +0.8
2,731,048 - clpB b2592 0.55 +0.5
2,731,121 - clpB b2592 0.58 +1.4
2,731,121 - clpB b2592 0.58 -0.0
2,731,133 + clpB b2592 0.59 -0.2
2,731,156 - clpB b2592 0.60 -1.2
2,731,267 - clpB b2592 0.64 -1.5
2,731,267 - clpB b2592 0.64 -1.8
2,731,351 + clpB b2592 0.67 +0.5
2,731,448 - clpB b2592 0.71 -1.8
2,731,468 + clpB b2592 0.72 -0.6
2,731,476 - clpB b2592 0.72 +0.9
2,731,476 - clpB b2592 0.72 -3.2
2,731,481 - clpB b2592 0.72 -1.8
2,731,531 - clpB b2592 0.74 +0.2
2,731,575 - clpB b2592 0.76 +0.1
2,731,674 - clpB b2592 0.80 +1.0
2,731,730 + clpB b2592 0.82 +0.6
2,731,736 - clpB b2592 0.82 +1.6
2,731,746 - clpB b2592 0.83 -2.1
2,731,746 - clpB b2592 0.83 +0.4
2,731,857 - clpB b2592 0.87 -1.2
2,731,868 + clpB b2592 0.87 +1.1
2,731,919 + clpB b2592 0.89 +0.9
2,731,927 - clpB b2592 0.90 +0.5
2,731,965 - +2.4
2,731,966 - -1.0
2,731,966 - +1.4
2,732,002 - -1.5
2,732,140 - +1.2
2,732,202 + ryfD b4609 0.19 -1.3
2,732,205 - ryfD b4609 0.21 +1.2
2,732,212 + ryfD b4609 0.26 +1.8
2,732,212 + ryfD b4609 0.26 +0.2
2,732,251 + ryfD b4609 0.53 +0.6
2,732,277 - ryfD b4609 0.71 -0.8
2,732,277 - ryfD b4609 0.71 -1.3
2,732,323 + -0.2
2,732,323 + -1.2
2,732,456 - yfiH b2593 0.18 -1.5
2,732,499 - yfiH b2593 0.24 -0.3
2,732,499 - yfiH b2593 0.24 -0.8
2,732,502 - yfiH b2593 0.24 +0.8
2,732,502 - yfiH b2593 0.24 +0.4
2,732,563 - yfiH b2593 0.33 +1.3
2,732,563 - yfiH b2593 0.33 -1.0
2,732,594 + yfiH b2593 0.37 -0.6
2,732,602 - yfiH b2593 0.38 +1.2
2,732,648 + yfiH b2593 0.44 -0.6
2,732,659 + yfiH b2593 0.46 +0.4
2,732,669 + yfiH b2593 0.47 +0.2
2,732,748 + yfiH b2593 0.58 -0.4
2,732,756 - yfiH b2593 0.59 +0.4
2,732,763 - yfiH b2593 0.60 -2.2
2,732,763 - yfiH b2593 0.60 -1.5
2,732,778 + yfiH b2593 0.62 -0.8
2,732,813 - yfiH b2593 0.67 -0.6
2,732,813 - yfiH b2593 0.67 -1.3
2,732,852 - yfiH b2593 0.72 +0.3
2,732,854 - yfiH b2593 0.72 +0.5
2,732,926 + yfiH b2593 0.82 -0.3
2,732,943 - yfiH b2593 0.84 -1.5
2,732,969 - yfiH b2593 0.88 -0.5
2,732,969 - yfiH b2593 0.88 -1.2
2,732,994 + -1.9
2,733,052 + -0.1
2,733,060 - +0.6
2,733,062 - -1.0
2,733,072 + -0.0
2,733,079 - +1.7
2,733,079 - +0.1

Or see this region's nucleotide sequence