Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpA and mhpB are separated by 1 nucleotides mhpB and mhpC are separated by 2 nucleotides mhpC and mhpD are separated by 9 nucleotides
b0347: mhpA - 3-(3-hydroxyphenyl)propionate hydroxylase (NCBI), at 367,835 to 369,499
mhpA
b0348: mhpB - 2,3-dihydroxyphenylpropionate 1,2-dioxygenase (NCBI), at 369,501 to 370,445
mhpB
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
Position (kb) 369
370
371 Strain fitness (log2 ratio) -2
-1
0
1
2 at 368.568 kb on - strand, within mhpA at 368.661 kb on - strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.728 kb on + strand, within mhpA at 368.755 kb on + strand, within mhpA at 368.761 kb on - strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.763 kb on + strand, within mhpA at 368.778 kb on + strand, within mhpA at 368.883 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 368.898 kb on - strand, within mhpA at 369.036 kb on - strand, within mhpA at 369.077 kb on - strand, within mhpA at 369.133 kb on + strand, within mhpA at 369.232 kb on + strand, within mhpA at 369.283 kb on + strand, within mhpA at 369.418 kb on + strand at 369.625 kb on + strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.705 kb on - strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.829 kb on + strand, within mhpB at 369.837 kb on - strand, within mhpB at 369.908 kb on + strand, within mhpB at 369.908 kb on + strand, within mhpB at 370.056 kb on - strand, within mhpB at 370.198 kb on - strand, within mhpB at 370.263 kb on + strand, within mhpB at 370.344 kb on + strand, within mhpB at 370.364 kb on + strand at 370.398 kb on - strand at 370.517 kb on - strand at 370.524 kb on + strand at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.598 kb on - strand, within mhpC at 370.632 kb on + strand, within mhpC at 370.635 kb on - strand, within mhpC at 370.688 kb on - strand, within mhpC at 370.690 kb on + strand, within mhpC at 370.813 kb on + strand, within mhpC at 370.931 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.051 kb on - strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.089 kb on + strand, within mhpC at 371.143 kb on - strand, within mhpC at 371.194 kb on + strand, within mhpC at 371.196 kb on + strand, within mhpC at 371.237 kb on + strand, within mhpC at 371.247 kb on - strand at 371.280 kb on - strand at 371.325 kb on + strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.360 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM remove 368,568 - mhpA b0347 0.44 +0.2 368,661 - mhpA b0347 0.50 +1.0 368,728 + mhpA b0347 0.54 -0.5 368,728 + mhpA b0347 0.54 -0.6 368,755 + mhpA b0347 0.55 +0.0 368,761 - mhpA b0347 0.56 +0.8 368,763 + mhpA b0347 0.56 +1.7 368,763 + mhpA b0347 0.56 -0.9 368,778 + mhpA b0347 0.57 +1.2 368,883 - mhpA b0347 0.63 +1.2 368,898 - mhpA b0347 0.64 +2.0 368,898 - mhpA b0347 0.64 -1.4 369,036 - mhpA b0347 0.72 +0.1 369,077 - mhpA b0347 0.75 +0.2 369,133 + mhpA b0347 0.78 -0.4 369,232 + mhpA b0347 0.84 +1.4 369,283 + mhpA b0347 0.87 +0.1 369,418 + +1.1 369,625 + mhpB b0348 0.13 -1.0 369,705 - mhpB b0348 0.22 -0.6 369,705 - mhpB b0348 0.22 -1.0 369,829 + mhpB b0348 0.35 -0.4 369,829 + mhpB b0348 0.35 -1.4 369,837 - mhpB b0348 0.36 +0.5 369,908 + mhpB b0348 0.43 -0.0 369,908 + mhpB b0348 0.43 +0.1 370,056 - mhpB b0348 0.59 +0.4 370,198 - mhpB b0348 0.74 -1.9 370,263 + mhpB b0348 0.81 -0.1 370,344 + mhpB b0348 0.89 -0.2 370,364 + +1.0 370,398 - -0.1 370,517 - +0.5 370,524 + +0.5 370,598 - mhpC b0349 0.17 -0.1 370,598 - mhpC b0349 0.17 +1.5 370,598 - mhpC b0349 0.17 +1.8 370,632 + mhpC b0349 0.21 +1.0 370,635 - mhpC b0349 0.21 +0.3 370,688 - mhpC b0349 0.27 +0.1 370,690 + mhpC b0349 0.27 +0.0 370,813 + mhpC b0349 0.41 -0.3 370,931 - mhpC b0349 0.55 +0.1 371,051 - mhpC b0349 0.68 -1.5 371,051 - mhpC b0349 0.68 -0.2 371,089 + mhpC b0349 0.73 -2.0 371,089 + mhpC b0349 0.73 +0.4 371,143 - mhpC b0349 0.79 +0.4 371,194 + mhpC b0349 0.85 -1.0 371,196 + mhpC b0349 0.85 -1.3 371,237 + mhpC b0349 0.89 -1.4 371,247 - +0.1 371,280 - -0.2 371,325 + +0.6 371,331 - -0.6 371,331 - -1.2 371,331 - +0.8 371,360 - -1.9
Or see this region's nucleotide sequence