Strain Fitness in Escherichia coli BW25113 around b0025

Experiment: LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsA-1 and rpsT are separated by 306 nucleotidesrpsT and yaaY are separated by 102 nucleotidesyaaY and ribF are separated by 7 nucleotidesribF and ileS are separated by 42 nucleotides b0022: insA-1 - IS1 repressor protein InsA (NCBI), at 20,233 to 20,508 insA-1 b0023: rpsT - 30S ribosomal protein S20 (NCBI), at 20,815 to 21,078 rpsT b0024: yaaY - hypothetical protein (NCBI), at 21,181 to 21,399 yaaY b0025: ribF - hypothetical protein (NCBI), at 21,407 to 22,348 ribF b0026: ileS - isoleucyl-tRNA synthetase (NCBI), at 22,391 to 25,207 ileS Position (kb) 21 22 23Strain fitness (log2 ratio) -2 -1 0 1at 20.506 kb on + strandat 20.574 kb on - strandat 20.636 kb on + strandat 20.688 kb on + strandat 20.696 kb on - strandat 21.175 kb on + strandat 21.433 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Chlorhexidine diacetate salt 0.000000625 mM
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20,506 + +0.6
20,574 - -0.8
20,636 + +1.0
20,688 + +0.8
20,696 - +0.7
21,175 + +0.0
21,433 + -2.3

Or see this region's nucleotide sequence