Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgbU and sgbE overlap by 7 nucleotides sgbE and ysaC are separated by 31 nucleotides ysaC and ysaD are separated by 3 nucleotides ysaD and yiaT are separated by 18 nucleotides yiaT and yiaU are separated by 123 nucleotides
b3582: sgbU - probable 3-hexulose-6-phosphate isomerase (VIMSS), at 3,747,255 to 3,748,115
sgbU
b3583: sgbE - L-ribulose-5-phosphate 4-epimerase (NCBI), at 3,748,109 to 3,748,804
sgbE
b4648: ysaC - no description, at 3,748,836 to 3,748,937
ysaC
b4649: ysaD - no description, at 3,748,941 to 3,749,132
ysaD
b3584: yiaT - hypothetical protein (NCBI), at 3,749,151 to 3,749,891
yiaT
b3585: yiaU - predicted DNA-binding transcriptional regulator (NCBI), at 3,750,015 to 3,750,989
yiaU
Position (kb)
3748
3749
3750 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3748.022 kb on - strand, within sgbU at 3748.022 kb on - strand, within sgbU at 3748.034 kb on + strand at 3748.083 kb on - strand at 3748.103 kb on + strand at 3748.128 kb on - strand at 3748.226 kb on - strand, within sgbE at 3748.237 kb on + strand, within sgbE at 3748.237 kb on + strand, within sgbE at 3748.253 kb on + strand, within sgbE at 3748.276 kb on + strand, within sgbE at 3748.276 kb on + strand, within sgbE at 3748.277 kb on - strand, within sgbE at 3748.324 kb on + strand, within sgbE at 3748.324 kb on + strand, within sgbE at 3748.396 kb on - strand, within sgbE at 3748.479 kb on + strand, within sgbE at 3748.479 kb on + strand, within sgbE at 3748.487 kb on - strand, within sgbE at 3748.501 kb on + strand, within sgbE at 3748.501 kb on + strand, within sgbE at 3748.502 kb on - strand, within sgbE at 3748.530 kb on + strand, within sgbE at 3748.566 kb on + strand, within sgbE at 3748.636 kb on + strand, within sgbE at 3748.666 kb on - strand, within sgbE at 3748.700 kb on - strand, within sgbE at 3748.722 kb on - strand, within sgbE at 3748.821 kb on + strand at 3748.828 kb on + strand at 3748.836 kb on - strand at 3748.858 kb on + strand, within ysaC at 3748.858 kb on + strand, within ysaC at 3748.869 kb on + strand, within ysaC at 3748.869 kb on + strand, within ysaC at 3748.877 kb on - strand, within ysaC at 3748.930 kb on + strand at 3748.930 kb on + strand at 3748.959 kb on + strand at 3748.963 kb on + strand, within ysaD at 3748.976 kb on + strand, within ysaD at 3748.976 kb on + strand, within ysaD at 3749.047 kb on + strand, within ysaD at 3749.071 kb on + strand, within ysaD at 3749.081 kb on + strand, within ysaD at 3749.086 kb on + strand, within ysaD at 3749.166 kb on - strand at 3749.166 kb on - strand at 3749.188 kb on + strand at 3749.238 kb on + strand, within yiaT at 3749.243 kb on + strand, within yiaT at 3749.317 kb on - strand, within yiaT at 3749.317 kb on - strand, within yiaT at 3749.332 kb on + strand, within yiaT at 3749.332 kb on + strand, within yiaT at 3749.345 kb on - strand, within yiaT at 3749.350 kb on + strand, within yiaT at 3749.352 kb on - strand, within yiaT at 3749.352 kb on - strand, within yiaT at 3749.352 kb on - strand, within yiaT at 3749.469 kb on - strand, within yiaT at 3749.472 kb on + strand, within yiaT at 3749.472 kb on + strand, within yiaT at 3749.505 kb on + strand, within yiaT at 3749.505 kb on + strand, within yiaT at 3749.523 kb on + strand, within yiaT at 3749.523 kb on + strand, within yiaT at 3749.545 kb on + strand, within yiaT at 3749.553 kb on - strand, within yiaT at 3749.562 kb on + strand, within yiaT at 3749.562 kb on + strand, within yiaT at 3749.573 kb on + strand, within yiaT at 3749.575 kb on + strand, within yiaT at 3749.603 kb on + strand, within yiaT at 3749.616 kb on + strand, within yiaT at 3749.642 kb on + strand, within yiaT at 3749.642 kb on + strand, within yiaT at 3749.643 kb on + strand, within yiaT at 3749.643 kb on + strand at 3749.648 kb on + strand, within yiaT at 3749.654 kb on + strand, within yiaT at 3749.657 kb on - strand, within yiaT at 3749.657 kb on - strand, within yiaT at 3749.659 kb on + strand, within yiaT at 3749.690 kb on + strand, within yiaT at 3749.697 kb on + strand, within yiaT at 3749.701 kb on + strand, within yiaT at 3749.714 kb on - strand, within yiaT at 3749.717 kb on + strand, within yiaT at 3749.723 kb on - strand, within yiaT at 3749.725 kb on + strand, within yiaT at 3749.731 kb on + strand, within yiaT at 3749.732 kb on + strand, within yiaT at 3749.758 kb on + strand, within yiaT at 3749.788 kb on + strand, within yiaT at 3749.788 kb on + strand, within yiaT at 3749.789 kb on + strand, within yiaT at 3749.789 kb on + strand, within yiaT at 3749.804 kb on + strand, within yiaT at 3749.804 kb on + strand, within yiaT at 3749.804 kb on + strand, within yiaT at 3749.942 kb on - strand at 3750.088 kb on - strand at 3750.104 kb on - strand at 3750.104 kb on - strand at 3750.107 kb on - strand at 3750.107 kb on - strand at 3750.114 kb on - strand, within yiaU at 3750.114 kb on - strand, within yiaU at 3750.114 kb on - strand, within yiaU at 3750.114 kb on - strand, within yiaU at 3750.124 kb on - strand, within yiaU
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 3,748,022 - sgbU b3582 0.89 +1.1 3,748,022 - sgbU b3582 0.89 -0.4 3,748,034 + +0.2 3,748,083 - -0.3 3,748,103 + -0.6 3,748,128 - -1.3 3,748,226 - sgbE b3583 0.17 -0.3 3,748,237 + sgbE b3583 0.18 -0.3 3,748,237 + sgbE b3583 0.18 -0.4 3,748,253 + sgbE b3583 0.21 +0.1 3,748,276 + sgbE b3583 0.24 +0.7 3,748,276 + sgbE b3583 0.24 -0.3 3,748,277 - sgbE b3583 0.24 +0.4 3,748,324 + sgbE b3583 0.31 -0.1 3,748,324 + sgbE b3583 0.31 +0.1 3,748,396 - sgbE b3583 0.41 -0.1 3,748,479 + sgbE b3583 0.53 +0.2 3,748,479 + sgbE b3583 0.53 +0.4 3,748,487 - sgbE b3583 0.54 -0.1 3,748,501 + sgbE b3583 0.56 +0.9 3,748,501 + sgbE b3583 0.56 -1.4 3,748,502 - sgbE b3583 0.56 +0.7 3,748,530 + sgbE b3583 0.60 +0.2 3,748,566 + sgbE b3583 0.66 +0.2 3,748,636 + sgbE b3583 0.76 -0.6 3,748,666 - sgbE b3583 0.80 +0.7 3,748,700 - sgbE b3583 0.85 -0.4 3,748,722 - sgbE b3583 0.88 +1.1 3,748,821 + -1.6 3,748,828 + +0.1 3,748,836 - -0.4 3,748,858 + ysaC b4648 0.22 +0.7 3,748,858 + ysaC b4648 0.22 +1.7 3,748,869 + ysaC b4648 0.32 -0.3 3,748,869 + ysaC b4648 0.32 -0.7 3,748,877 - ysaC b4648 0.40 +0.6 3,748,930 + +0.3 3,748,930 + +0.0 3,748,959 + -0.6 3,748,963 + ysaD b4649 0.11 -0.1 3,748,976 + ysaD b4649 0.18 -2.3 3,748,976 + ysaD b4649 0.18 -0.3 3,749,047 + ysaD b4649 0.55 -0.5 3,749,071 + ysaD b4649 0.68 -2.5 3,749,081 + ysaD b4649 0.73 -0.7 3,749,086 + ysaD b4649 0.76 +0.1 3,749,166 - +0.4 3,749,166 - +0.4 3,749,188 + -1.0 3,749,238 + yiaT b3584 0.12 +0.7 3,749,243 + yiaT b3584 0.12 +0.7 3,749,317 - yiaT b3584 0.22 -1.4 3,749,317 - yiaT b3584 0.22 +0.7 3,749,332 + yiaT b3584 0.24 +1.2 3,749,332 + yiaT b3584 0.24 +0.3 3,749,345 - yiaT b3584 0.26 -0.3 3,749,350 + yiaT b3584 0.27 -0.3 3,749,352 - yiaT b3584 0.27 +1.2 3,749,352 - yiaT b3584 0.27 -2.1 3,749,352 - yiaT b3584 0.27 +0.4 3,749,469 - yiaT b3584 0.43 -0.9 3,749,472 + yiaT b3584 0.43 -0.4 3,749,472 + yiaT b3584 0.43 +0.3 3,749,505 + yiaT b3584 0.48 +0.7 3,749,505 + yiaT b3584 0.48 +1.7 3,749,523 + yiaT b3584 0.50 +1.0 3,749,523 + yiaT b3584 0.50 +0.2 3,749,545 + yiaT b3584 0.53 +0.7 3,749,553 - yiaT b3584 0.54 +0.3 3,749,562 + yiaT b3584 0.55 +2.7 3,749,562 + yiaT b3584 0.55 -0.6 3,749,573 + yiaT b3584 0.57 -0.3 3,749,575 + yiaT b3584 0.57 +0.0 3,749,603 + yiaT b3584 0.61 +0.2 3,749,616 + yiaT b3584 0.63 -0.3 3,749,642 + yiaT b3584 0.66 -0.4 3,749,642 + yiaT b3584 0.66 +0.2 3,749,643 + yiaT b3584 0.66 -0.3 3,749,643 + +0.7 3,749,648 + yiaT b3584 0.67 +1.7 3,749,654 + yiaT b3584 0.68 -0.3 3,749,657 - yiaT b3584 0.68 +0.2 3,749,657 - yiaT b3584 0.68 +1.7 3,749,659 + yiaT b3584 0.69 -0.6 3,749,690 + yiaT b3584 0.73 +0.5 3,749,697 + yiaT b3584 0.74 +1.5 3,749,701 + yiaT b3584 0.74 -0.3 3,749,714 - yiaT b3584 0.76 +1.7 3,749,717 + yiaT b3584 0.76 +0.2 3,749,723 - yiaT b3584 0.77 +0.4 3,749,725 + yiaT b3584 0.77 +2.7 3,749,731 + yiaT b3584 0.78 +1.4 3,749,732 + yiaT b3584 0.78 -0.7 3,749,758 + yiaT b3584 0.82 +0.7 3,749,788 + yiaT b3584 0.86 -1.1 3,749,788 + yiaT b3584 0.86 +1.2 3,749,789 + yiaT b3584 0.86 -0.3 3,749,789 + yiaT b3584 0.86 -0.3 3,749,804 + yiaT b3584 0.88 +0.2 3,749,804 + yiaT b3584 0.88 -0.3 3,749,804 + yiaT b3584 0.88 -0.6 3,749,942 - +1.7 3,750,088 - +0.2 3,750,104 - +0.2 3,750,104 - +1.7 3,750,107 - +0.2 3,750,107 - -0.8 3,750,114 - yiaU b3585 0.10 +1.7 3,750,114 - yiaU b3585 0.10 +1.7 3,750,114 - yiaU b3585 0.10 -0.6 3,750,114 - yiaU b3585 0.10 -0.6 3,750,124 - yiaU b3585 0.11 +0.4
Or see this region's nucleotide sequence