Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yjgN and valS are separated by 55 nucleotides valS and holC overlap by 1 nucleotides holC and pepA are separated by 159 nucleotides
b4257: yjgN - conserved inner membrane protein (NCBI), at 4,477,753 to 4,478,949
yjgN
b4258: valS - valyl-tRNA synthetase (NCBI), at 4,479,005 to 4,481,860
valS
b4259: holC - DNA polymerase III subunit chi (NCBI), at 4,481,860 to 4,482,303
holC
b4260: pepA - leucyl aminopeptidase (NCBI), at 4,482,463 to 4,483,974
pepA
Position (kb)
4479
4480
4481
4482 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4478.268 kb on + strand, within yjgN at 4478.530 kb on + strand, within yjgN at 4478.668 kb on + strand, within yjgN at 4478.675 kb on + strand, within yjgN at 4478.675 kb on + strand, within yjgN at 4478.678 kb on - strand, within yjgN at 4478.702 kb on - strand, within yjgN at 4478.702 kb on - strand, within yjgN at 4478.726 kb on + strand, within yjgN at 4478.727 kb on + strand, within yjgN at 4478.727 kb on + strand, within yjgN at 4478.727 kb on + strand, within yjgN at 4478.741 kb on + strand, within yjgN at 4478.744 kb on + strand, within yjgN at 4478.747 kb on + strand, within yjgN at 4478.750 kb on + strand, within yjgN at 4478.770 kb on + strand, within yjgN at 4478.770 kb on - strand, within yjgN at 4478.780 kb on + strand, within yjgN at 4478.784 kb on + strand, within yjgN at 4478.789 kb on + strand, within yjgN at 4478.858 kb on + strand at 4478.858 kb on + strand at 4478.898 kb on - strand at 4478.926 kb on + strand at 4478.926 kb on + strand at 4478.930 kb on + strand at 4482.341 kb on + strand at 4482.349 kb on - strand at 4482.354 kb on - strand at 4482.369 kb on - strand at 4482.369 kb on - strand at 4482.379 kb on - strand at 4482.394 kb on + strand at 4482.394 kb on - strand at 4482.402 kb on - strand at 4482.402 kb on - strand at 4482.404 kb on + strand at 4482.445 kb on + strand at 4482.452 kb on + strand at 4482.458 kb on + strand at 4482.460 kb on + strand at 4482.494 kb on + strand at 4482.582 kb on - strand at 4482.650 kb on + strand, within pepA at 4482.690 kb on - strand, within pepA at 4482.698 kb on - strand, within pepA at 4482.706 kb on + strand at 4482.706 kb on + strand at 4482.726 kb on - strand, within pepA at 4482.741 kb on + strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.749 kb on - strand, within pepA at 4482.787 kb on + strand, within pepA at 4482.787 kb on + strand, within pepA at 4482.800 kb on - strand, within pepA at 4482.805 kb on - strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA at 4482.822 kb on + strand, within pepA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 4,478,268 + yjgN b4257 0.43 +1.5 4,478,530 + yjgN b4257 0.65 +1.5 4,478,668 + yjgN b4257 0.76 -0.8 4,478,675 + yjgN b4257 0.77 +3.1 4,478,675 + yjgN b4257 0.77 +2.1 4,478,678 - yjgN b4257 0.77 +1.5 4,478,702 - yjgN b4257 0.79 +1.5 4,478,702 - yjgN b4257 0.79 -0.1 4,478,726 + yjgN b4257 0.81 -0.5 4,478,727 + yjgN b4257 0.81 +1.1 4,478,727 + yjgN b4257 0.81 -0.4 4,478,727 + yjgN b4257 0.81 -1.1 4,478,741 + yjgN b4257 0.83 -0.5 4,478,744 + yjgN b4257 0.83 -0.3 4,478,747 + yjgN b4257 0.83 -1.0 4,478,750 + yjgN b4257 0.83 +0.5 4,478,770 + yjgN b4257 0.85 +0.7 4,478,770 - yjgN b4257 0.85 +1.5 4,478,780 + yjgN b4257 0.86 -0.0 4,478,784 + yjgN b4257 0.86 +0.1 4,478,789 + yjgN b4257 0.87 -0.7 4,478,858 + -0.5 4,478,858 + +0.3 4,478,898 - +0.3 4,478,926 + +0.8 4,478,926 + +0.1 4,478,930 + +0.7 4,482,341 + -0.3 4,482,349 - -0.8 4,482,354 - -0.6 4,482,369 - -0.4 4,482,369 - -0.3 4,482,379 - -0.1 4,482,394 + -0.9 4,482,394 - +0.0 4,482,402 - -0.3 4,482,402 - -0.4 4,482,404 + -0.0 4,482,445 + -0.8 4,482,452 + -1.1 4,482,458 + +0.5 4,482,460 + -0.6 4,482,494 + -0.1 4,482,582 - -1.0 4,482,650 + pepA b4260 0.12 +0.1 4,482,690 - pepA b4260 0.15 -1.1 4,482,698 - pepA b4260 0.16 -2.4 4,482,706 + -0.1 4,482,706 + +1.5 4,482,726 - pepA b4260 0.17 -0.1 4,482,741 + pepA b4260 0.18 +0.7 4,482,749 - pepA b4260 0.19 -1.3 4,482,749 - pepA b4260 0.19 +0.2 4,482,749 - pepA b4260 0.19 +0.2 4,482,787 + pepA b4260 0.21 -0.1 4,482,787 + pepA b4260 0.21 -1.8 4,482,800 - pepA b4260 0.22 +0.8 4,482,805 - pepA b4260 0.23 -0.7 4,482,822 + pepA b4260 0.24 -0.1 4,482,822 + pepA b4260 0.24 +0.5 4,482,822 + pepA b4260 0.24 +0.3 4,482,822 + pepA b4260 0.24 -0.5
Or see this region's nucleotide sequence