Strain Fitness in Escherichia coli BW25113 around b3653

Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecG and gltS are separated by 168 nucleotidesgltS and yicE are separated by 279 nucleotides b3652: recG - ATP-dependent DNA helicase (NCBI), at 3,823,233 to 3,825,314 recG b3653: gltS - glutamate transporter (NCBI), at 3,825,483 to 3,826,688 gltS b3654: yicE - predicted transporter (NCBI), at 3,826,968 to 3,828,359 yicE Position (kb) 3825 3826 3827Strain fitness (log2 ratio) -2 -1 0 1at 3824.594 kb on - strand, within recGat 3824.609 kb on - strand, within recGat 3824.653 kb on + strand, within recGat 3824.658 kb on + strand, within recGat 3824.661 kb on - strand, within recGat 3824.666 kb on + strand, within recGat 3824.681 kb on - strand, within recGat 3824.681 kb on - strand, within recGat 3824.751 kb on + strand, within recGat 3824.793 kb on + strand, within recGat 3824.799 kb on + strand, within recGat 3824.799 kb on + strand, within recGat 3824.868 kb on - strand, within recGat 3824.876 kb on + strand, within recGat 3824.878 kb on + strand, within recGat 3824.923 kb on - strand, within recGat 3824.983 kb on + strand, within recGat 3824.983 kb on + strand, within recGat 3825.011 kb on - strand, within recGat 3825.011 kb on - strand, within recGat 3825.013 kb on - strand, within recGat 3825.038 kb on + strand, within recGat 3825.038 kb on + strand, within recGat 3825.218 kb on + strandat 3825.253 kb on - strandat 3825.334 kb on - strandat 3825.334 kb on - strandat 3825.354 kb on - strandat 3825.364 kb on - strandat 3825.414 kb on + strandat 3825.505 kb on + strandat 3825.505 kb on + strandat 3825.593 kb on + strandat 3825.593 kb on + strandat 3825.602 kb on + strandat 3825.650 kb on + strand, within gltSat 3825.711 kb on - strand, within gltSat 3825.716 kb on - strand, within gltSat 3825.756 kb on - strand, within gltSat 3825.803 kb on + strand, within gltSat 3825.834 kb on - strand, within gltSat 3825.850 kb on - strand, within gltSat 3825.855 kb on - strand, within gltSat 3825.860 kb on - strand, within gltSat 3825.870 kb on - strand, within gltSat 3825.932 kb on + strand, within gltSat 3825.932 kb on + strand, within gltSat 3826.037 kb on - strand, within gltSat 3826.070 kb on - strand, within gltSat 3826.140 kb on - strand, within gltSat 3826.173 kb on + strand, within gltSat 3826.173 kb on + strand, within gltSat 3826.208 kb on - strand, within gltSat 3826.212 kb on - strand, within gltSat 3826.273 kb on - strand, within gltSat 3826.275 kb on - strand, within gltSat 3826.289 kb on + strand, within gltSat 3826.297 kb on - strand, within gltSat 3826.345 kb on - strand, within gltSat 3826.446 kb on + strand, within gltSat 3826.446 kb on + strand, within gltSat 3826.488 kb on + strand, within gltSat 3826.488 kb on + strand, within gltSat 3826.489 kb on + strand, within gltSat 3826.511 kb on + strand, within gltSat 3826.556 kb on + strand, within gltSat 3826.578 kb on + strandat 3826.596 kb on - strandat 3826.596 kb on - strandat 3826.622 kb on + strandat 3826.631 kb on - strandat 3826.634 kb on + strandat 3826.663 kb on - strandat 3826.663 kb on - strandat 3826.733 kb on - strandat 3826.733 kb on - strandat 3826.752 kb on - strandat 3826.759 kb on + strandat 3826.760 kb on + strandat 3826.760 kb on + strandat 3827.075 kb on + strandat 3827.107 kb on + strandat 3827.107 kb on + strandat 3827.115 kb on - strand, within yicEat 3827.145 kb on + strand, within yicEat 3827.145 kb on + strand, within yicEat 3827.148 kb on - strand, within yicEat 3827.179 kb on + strand, within yicEat 3827.203 kb on + strand, within yicEat 3827.266 kb on - strand, within yicEat 3827.319 kb on - strand, within yicEat 3827.326 kb on - strand, within yicEat 3827.326 kb on - strand, within yicEat 3827.374 kb on - strand, within yicEat 3827.379 kb on - strand, within yicEat 3827.386 kb on + strand, within yicEat 3827.417 kb on + strand, within yicEat 3827.478 kb on - strand, within yicEat 3827.478 kb on - strand, within yicEat 3827.524 kb on - strand, within yicEat 3827.532 kb on + strand, within yicEat 3827.588 kb on - strand, within yicEat 3827.588 kb on - strand, within yicEat 3827.590 kb on + strand, within yicEat 3827.607 kb on + strand, within yicEat 3827.607 kb on + strand, within yicEat 3827.634 kb on + strand, within yicEat 3827.684 kb on + strand, within yicEat 3827.686 kb on - strand, within yicE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
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3,824,594 - recG b3652 0.65 +0.4
3,824,609 - recG b3652 0.66 +0.7
3,824,653 + recG b3652 0.68 -0.8
3,824,658 + recG b3652 0.68 +1.7
3,824,661 - recG b3652 0.69 -1.6
3,824,666 + recG b3652 0.69 +0.2
3,824,681 - recG b3652 0.70 +0.9
3,824,681 - recG b3652 0.70 -0.6
3,824,751 + recG b3652 0.73 +1.7
3,824,793 + recG b3652 0.75 -0.1
3,824,799 + recG b3652 0.75 -0.6
3,824,799 + recG b3652 0.75 +0.7
3,824,868 - recG b3652 0.79 -1.1
3,824,876 + recG b3652 0.79 -0.3
3,824,878 + recG b3652 0.79 +1.7
3,824,923 - recG b3652 0.81 -1.4
3,824,983 + recG b3652 0.84 +1.7
3,824,983 + recG b3652 0.84 +0.7
3,825,011 - recG b3652 0.85 +0.7
3,825,011 - recG b3652 0.85 +1.2
3,825,013 - recG b3652 0.85 -1.0
3,825,038 + recG b3652 0.87 +0.7
3,825,038 + recG b3652 0.87 -0.3
3,825,218 + -0.1
3,825,253 - -2.0
3,825,334 - +0.2
3,825,334 - +0.4
3,825,354 - -0.5
3,825,364 - -0.8
3,825,414 + -0.3
3,825,505 + -0.3
3,825,505 + -0.3
3,825,593 + +0.2
3,825,593 + -0.2
3,825,602 + -0.3
3,825,650 + gltS b3653 0.14 -0.4
3,825,711 - gltS b3653 0.19 -0.4
3,825,716 - gltS b3653 0.19 -0.7
3,825,756 - gltS b3653 0.23 +0.4
3,825,803 + gltS b3653 0.27 +0.9
3,825,834 - gltS b3653 0.29 +0.3
3,825,850 - gltS b3653 0.30 -0.4
3,825,855 - gltS b3653 0.31 -0.6
3,825,860 - gltS b3653 0.31 -0.7
3,825,870 - gltS b3653 0.32 -0.9
3,825,932 + gltS b3653 0.37 +0.6
3,825,932 + gltS b3653 0.37 +0.2
3,826,037 - gltS b3653 0.46 -0.4
3,826,070 - gltS b3653 0.49 -0.0
3,826,140 - gltS b3653 0.54 -0.3
3,826,173 + gltS b3653 0.57 -0.8
3,826,173 + gltS b3653 0.57 +0.3
3,826,208 - gltS b3653 0.60 -0.4
3,826,212 - gltS b3653 0.60 -0.8
3,826,273 - gltS b3653 0.66 -0.5
3,826,275 - gltS b3653 0.66 -0.6
3,826,289 + gltS b3653 0.67 -0.6
3,826,297 - gltS b3653 0.67 +0.7
3,826,345 - gltS b3653 0.71 -1.3
3,826,446 + gltS b3653 0.80 +0.6
3,826,446 + gltS b3653 0.80 +1.5
3,826,488 + gltS b3653 0.83 -0.5
3,826,488 + gltS b3653 0.83 +0.2
3,826,489 + gltS b3653 0.83 +0.4
3,826,511 + gltS b3653 0.85 -0.2
3,826,556 + gltS b3653 0.89 -2.2
3,826,578 + +0.1
3,826,596 - -1.3
3,826,596 - -0.1
3,826,622 + -0.2
3,826,631 - +0.5
3,826,634 + +0.3
3,826,663 - +0.6
3,826,663 - +1.7
3,826,733 - -0.1
3,826,733 - -2.5
3,826,752 - -0.4
3,826,759 + +0.4
3,826,760 + -0.7
3,826,760 + +1.2
3,827,075 + +0.3
3,827,107 + +0.2
3,827,107 + -1.7
3,827,115 - yicE b3654 0.11 +0.4
3,827,145 + yicE b3654 0.13 -0.3
3,827,145 + yicE b3654 0.13 +1.7
3,827,148 - yicE b3654 0.13 -0.7
3,827,179 + yicE b3654 0.15 -1.1
3,827,203 + yicE b3654 0.17 +0.2
3,827,266 - yicE b3654 0.21 -0.2
3,827,319 - yicE b3654 0.25 -0.1
3,827,326 - yicE b3654 0.26 +0.2
3,827,326 - yicE b3654 0.26 -0.2
3,827,374 - yicE b3654 0.29 -0.2
3,827,379 - yicE b3654 0.30 -0.3
3,827,386 + yicE b3654 0.30 +0.8
3,827,417 + yicE b3654 0.32 -0.0
3,827,478 - yicE b3654 0.37 +0.2
3,827,478 - yicE b3654 0.37 -0.5
3,827,524 - yicE b3654 0.40 +0.6
3,827,532 + yicE b3654 0.41 -0.1
3,827,588 - yicE b3654 0.45 +0.3
3,827,588 - yicE b3654 0.45 -0.6
3,827,590 + yicE b3654 0.45 -0.2
3,827,607 + yicE b3654 0.46 -1.2
3,827,607 + yicE b3654 0.46 -0.4
3,827,634 + yicE b3654 0.48 +0.4
3,827,684 + yicE b3654 0.51 -0.7
3,827,686 - yicE b3654 0.52 +1.3

Or see this region's nucleotide sequence