Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sohA and yhaV overlap by 1 nucleotides yhaV and agaR are separated by 54 nucleotides agaR and kbaZ are separated by 248 nucleotides
b3129: sohA - predicted regulator (NCBI), at 3,275,024 to 3,275,359
sohA
b3130: yhaV - hypothetical protein (NCBI), at 3,275,359 to 3,275,823
yhaV
b3131: agaR - DNA-binding transcriptional dual regulator (NCBI), at 3,275,878 to 3,276,687
agaR
b3132: kbaZ - tagatose 6-phosphate aldolase 1, kbaZ subunit (NCBI), at 3,276,936 to 3,278,216
kbaZ
Position (kb)
3275
3276
3277 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3275.046 kb on + strand at 3275.046 kb on + strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.049 kb on - strand at 3275.051 kb on + strand at 3275.051 kb on + strand at 3275.057 kb on - strand at 3275.059 kb on - strand, within sohA at 3275.063 kb on + strand, within sohA at 3275.098 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.108 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.118 kb on + strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.318 kb on - strand, within sohA at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.392 kb on + strand at 3275.400 kb on - strand at 3275.400 kb on - strand at 3275.420 kb on - strand, within yhaV at 3275.427 kb on + strand, within yhaV at 3275.451 kb on + strand, within yhaV at 3275.512 kb on + strand, within yhaV at 3275.536 kb on + strand, within yhaV at 3275.596 kb on + strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.642 kb on - strand, within yhaV at 3275.678 kb on + strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.685 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.686 kb on - strand, within yhaV at 3275.690 kb on - strand, within yhaV at 3275.735 kb on + strand, within yhaV at 3275.741 kb on - strand, within yhaV at 3275.781 kb on - strand at 3275.781 kb on - strand at 3276.109 kb on + strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.131 kb on - strand, within agaR at 3276.170 kb on + strand, within agaR at 3276.242 kb on - strand, within agaR at 3276.242 kb on - strand at 3276.270 kb on + strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.447 kb on - strand, within agaR at 3276.560 kb on - strand, within agaR at 3276.602 kb on - strand, within agaR at 3276.625 kb on - strand at 3276.635 kb on - strand at 3276.643 kb on + strand at 3276.762 kb on - strand at 3276.790 kb on - strand at 3277.028 kb on - strand at 3277.120 kb on - strand, within kbaZ at 3277.220 kb on - strand, within kbaZ at 3277.261 kb on - strand, within kbaZ at 3277.338 kb on - strand, within kbaZ at 3277.338 kb on - strand, within kbaZ at 3277.393 kb on + strand, within kbaZ at 3277.393 kb on + strand, within kbaZ at 3277.419 kb on + strand, within kbaZ at 3277.443 kb on - strand, within kbaZ at 3277.443 kb on - strand, within kbaZ at 3277.477 kb on + strand, within kbaZ at 3277.602 kb on - strand, within kbaZ at 3277.603 kb on + strand, within kbaZ at 3277.603 kb on + strand, within kbaZ at 3277.647 kb on + strand, within kbaZ at 3277.647 kb on + strand, within kbaZ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 3,275,046 + -0.4 3,275,046 + +0.3 3,275,049 - +1.1 3,275,049 - +0.5 3,275,049 - -0.3 3,275,051 + +0.4 3,275,051 + +3.0 3,275,057 - +0.7 3,275,059 - sohA b3129 0.10 +1.9 3,275,063 + sohA b3129 0.12 +0.7 3,275,098 + sohA b3129 0.22 +0.9 3,275,108 + sohA b3129 0.25 -1.7 3,275,108 + sohA b3129 0.25 +0.7 3,275,118 + sohA b3129 0.28 +1.5 3,275,118 + sohA b3129 0.28 +0.5 3,275,318 - sohA b3129 0.88 +0.1 3,275,318 - sohA b3129 0.88 -0.1 3,275,392 + -0.2 3,275,392 + +0.0 3,275,392 + -0.8 3,275,400 - -2.1 3,275,400 - +1.5 3,275,420 - yhaV b3130 0.13 +0.1 3,275,427 + yhaV b3130 0.15 +0.2 3,275,451 + yhaV b3130 0.20 -0.0 3,275,512 + yhaV b3130 0.33 +1.0 3,275,536 + yhaV b3130 0.38 +0.1 3,275,596 + yhaV b3130 0.51 -0.0 3,275,642 - yhaV b3130 0.61 +0.5 3,275,642 - yhaV b3130 0.61 -0.5 3,275,678 + yhaV b3130 0.69 -1.2 3,275,685 - yhaV b3130 0.70 -0.7 3,275,685 - yhaV b3130 0.70 +0.5 3,275,686 - yhaV b3130 0.70 +1.9 3,275,686 - yhaV b3130 0.70 -0.5 3,275,690 - yhaV b3130 0.71 +1.2 3,275,735 + yhaV b3130 0.81 +3.8 3,275,741 - yhaV b3130 0.82 +0.1 3,275,781 - -0.5 3,275,781 - +0.6 3,276,109 + agaR b3131 0.29 -1.1 3,276,131 - agaR b3131 0.31 -1.0 3,276,131 - agaR b3131 0.31 -1.1 3,276,131 - agaR b3131 0.31 +0.0 3,276,170 + agaR b3131 0.36 -1.0 3,276,242 - agaR b3131 0.45 -0.3 3,276,242 - +1.5 3,276,270 + agaR b3131 0.48 -1.1 3,276,447 - agaR b3131 0.70 -0.4 3,276,447 - agaR b3131 0.70 -1.5 3,276,560 - agaR b3131 0.84 -1.7 3,276,602 - agaR b3131 0.89 -2.4 3,276,625 - -2.3 3,276,635 - -0.9 3,276,643 + -1.2 3,276,762 - +1.0 3,276,790 - +1.0 3,277,028 - +1.5 3,277,120 - kbaZ b3132 0.14 -0.4 3,277,220 - kbaZ b3132 0.22 +0.5 3,277,261 - kbaZ b3132 0.25 -0.0 3,277,338 - kbaZ b3132 0.31 +0.8 3,277,338 - kbaZ b3132 0.31 -0.8 3,277,393 + kbaZ b3132 0.36 +0.9 3,277,393 + kbaZ b3132 0.36 +0.7 3,277,419 + kbaZ b3132 0.38 +0.3 3,277,443 - kbaZ b3132 0.40 +0.0 3,277,443 - kbaZ b3132 0.40 +0.7 3,277,477 + kbaZ b3132 0.42 +0.6 3,277,602 - kbaZ b3132 0.52 -0.5 3,277,603 + kbaZ b3132 0.52 +0.1 3,277,603 + kbaZ b3132 0.52 -0.5 3,277,647 + kbaZ b3132 0.56 -0.5 3,277,647 + kbaZ b3132 0.56 -0.4
Or see this region's nucleotide sequence