Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sufD and sufC overlap by 26 nucleotides sufC and ynhE are separated by 9 nucleotides ynhE and sufA are separated by 8 nucleotides sufA and rydB are separated by 326 nucleotides rydB and ydiH are separated by 153 nucleotides
b1681: sufD - component of SufBCD complex (NCBI), at 1,758,544 to 1,759,815
sufD
b1682: sufC - cysteine desulfurase ATPase component (NCBI), at 1,759,790 to 1,760,536
sufC
b1683: ynhE - orf, hypothetical protein (VIMSS), at 1,760,546 to 1,762,033
ynhE
b1684: sufA - iron-sulfur cluster assembly scaffold protein (NCBI), at 1,762,042 to 1,762,410
sufA
b4430: rydB - regulatory sRNA (NCBI), at 1,762,737 to 1,762,804
rydB
b1685: ydiH - predicted protein (RefSeq), at 1,762,958 to 1,763,146
ydiH
Position (kb)
1760
1761
1762
1763 Strain fitness (log2 ratio)
-2
-1
0
1 at 1759.576 kb on - strand, within sufD at 1759.578 kb on - strand, within sufD at 1759.578 kb on - strand, within sufD at 1759.590 kb on - strand, within sufD at 1759.590 kb on - strand, within sufD at 1759.624 kb on + strand, within sufD at 1759.624 kb on + strand, within sufD at 1759.806 kb on - strand at 1759.806 kb on - strand at 1759.812 kb on + strand at 1759.812 kb on + strand at 1759.877 kb on + strand, within sufC at 1759.902 kb on - strand, within sufC at 1759.902 kb on - strand, within sufC at 1759.907 kb on - strand, within sufC at 1759.944 kb on + strand, within sufC at 1759.944 kb on + strand, within sufC at 1759.999 kb on + strand, within sufC at 1760.009 kb on - strand, within sufC at 1760.777 kb on - strand, within ynhE at 1760.788 kb on - strand, within ynhE at 1760.968 kb on - strand, within ynhE at 1760.968 kb on - strand, within ynhE at 1761.252 kb on - strand, within ynhE at 1761.371 kb on + strand, within ynhE at 1761.462 kb on + strand, within ynhE at 1761.662 kb on - strand, within ynhE at 1761.706 kb on - strand, within ynhE at 1761.706 kb on - strand, within ynhE at 1761.706 kb on - strand, within ynhE at 1761.721 kb on - strand, within ynhE at 1761.721 kb on - strand, within ynhE at 1761.867 kb on + strand, within ynhE at 1761.868 kb on - strand, within ynhE at 1762.235 kb on - strand, within sufA at 1762.235 kb on - strand, within sufA at 1762.245 kb on - strand, within sufA at 1762.437 kb on + strand at 1762.521 kb on + strand at 1762.546 kb on - strand at 1762.891 kb on - strand at 1762.891 kb on - strand at 1762.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 1,759,576 - sufD b1681 0.81 -0.8 1,759,578 - sufD b1681 0.81 -0.6 1,759,578 - sufD b1681 0.81 -0.4 1,759,590 - sufD b1681 0.82 -0.2 1,759,590 - sufD b1681 0.82 -1.3 1,759,624 + sufD b1681 0.85 -2.1 1,759,624 + sufD b1681 0.85 -0.3 1,759,806 - -1.1 1,759,806 - -1.8 1,759,812 + -2.3 1,759,812 + -0.8 1,759,877 + sufC b1682 0.12 -1.8 1,759,902 - sufC b1682 0.15 -0.8 1,759,902 - sufC b1682 0.15 -1.0 1,759,907 - sufC b1682 0.16 -2.1 1,759,944 + sufC b1682 0.21 -0.3 1,759,944 + sufC b1682 0.21 +0.0 1,759,999 + sufC b1682 0.28 -0.8 1,760,009 - sufC b1682 0.29 -1.1 1,760,777 - ynhE b1683 0.16 -1.6 1,760,788 - ynhE b1683 0.16 +0.0 1,760,968 - ynhE b1683 0.28 -2.7 1,760,968 - ynhE b1683 0.28 -1.7 1,761,252 - ynhE b1683 0.47 -1.5 1,761,371 + ynhE b1683 0.55 -1.3 1,761,462 + ynhE b1683 0.62 +0.3 1,761,662 - ynhE b1683 0.75 -2.1 1,761,706 - ynhE b1683 0.78 +0.0 1,761,706 - ynhE b1683 0.78 -0.8 1,761,706 - ynhE b1683 0.78 -1.6 1,761,721 - ynhE b1683 0.79 -0.4 1,761,721 - ynhE b1683 0.79 +0.2 1,761,867 + ynhE b1683 0.89 +0.2 1,761,868 - ynhE b1683 0.89 -0.6 1,762,235 - sufA b1684 0.52 -0.6 1,762,235 - sufA b1684 0.52 -1.2 1,762,245 - sufA b1684 0.55 -0.4 1,762,437 + -0.3 1,762,521 + -0.7 1,762,546 - +0.1 1,762,891 - -1.8 1,762,891 - +0.5 1,762,904 - +0.3
Or see this region's nucleotide sequence