Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnS and pncB are separated by 168 nucleotides pncB and pepN are separated by 265 nucleotides
b0930: asnS - asparaginyl-tRNA synthetase (NCBI), at 986,808 to 988,208
asnS
b0931: pncB - nicotinate phosphoribosyltransferase (NCBI), at 988,377 to 989,579
pncB
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
Position (kb) 988
989
990 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6
7 at 988.280 kb on - strand at 988.301 kb on + strand at 988.411 kb on + strand at 988.419 kb on - strand at 988.419 kb on - strand at 988.494 kb on + strand at 988.500 kb on - strand, within pncB at 988.654 kb on + strand at 988.670 kb on - strand, within pncB at 988.752 kb on - strand, within pncB at 988.752 kb on - strand, within pncB at 988.819 kb on + strand, within pncB at 988.819 kb on + strand, within pncB at 988.883 kb on + strand, within pncB at 989.105 kb on - strand, within pncB at 989.105 kb on - strand, within pncB at 989.167 kb on + strand, within pncB at 989.401 kb on + strand, within pncB at 989.401 kb on + strand, within pncB at 989.403 kb on - strand, within pncB at 989.445 kb on + strand, within pncB at 989.445 kb on + strand, within pncB at 989.493 kb on - strand at 989.493 kb on - strand at 989.541 kb on - strand at 989.541 kb on - strand at 989.718 kb on + strand at 989.726 kb on - strand at 989.726 kb on - strand at 989.771 kb on + strand at 989.771 kb on + strand at 989.771 kb on + strand at 989.798 kb on + strand at 989.809 kb on - strand at 989.810 kb on + strand at 989.819 kb on - strand at 989.819 kb on - strand at 989.828 kb on - strand at 990.041 kb on + strand at 990.059 kb on - strand at 990.142 kb on - strand, within pepN at 990.147 kb on + strand, within pepN at 990.147 kb on + strand, within pepN at 990.201 kb on + strand, within pepN at 990.209 kb on - strand, within pepN at 990.209 kb on - strand, within pepN at 990.357 kb on + strand, within pepN at 990.357 kb on + strand, within pepN at 990.433 kb on - strand, within pepN at 990.433 kb on - strand, within pepN at 990.499 kb on + strand, within pepN at 990.554 kb on + strand, within pepN at 990.554 kb on + strand, within pepN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 988,280 - +0.1 988,301 + +0.4 988,411 + -0.1 988,419 - -1.8 988,419 - -0.9 988,494 + -1.5 988,500 - pncB b0931 0.10 +0.9 988,654 + -0.1 988,670 - pncB b0931 0.24 +0.1 988,752 - pncB b0931 0.31 -0.8 988,752 - pncB b0931 0.31 -1.5 988,819 + pncB b0931 0.37 -2.9 988,819 + pncB b0931 0.37 -1.5 988,883 + pncB b0931 0.42 -0.7 989,105 - pncB b0931 0.61 -0.7 989,105 - pncB b0931 0.61 +0.1 989,167 + pncB b0931 0.66 +1.9 989,401 + pncB b0931 0.85 -1.1 989,401 + pncB b0931 0.85 -0.1 989,403 - pncB b0931 0.85 -0.5 989,445 + pncB b0931 0.89 -0.8 989,445 + pncB b0931 0.89 -1.8 989,493 - -1.0 989,493 - -1.9 989,541 - -0.9 989,541 - -2.3 989,718 + -1.1 989,726 - +0.1 989,726 - +0.6 989,771 + -1.3 989,771 + +0.4 989,771 + -0.7 989,798 + -0.4 989,809 - +0.9 989,810 + +0.1 989,819 - -1.0 989,819 - +1.2 989,828 - -0.7 990,041 + +7.3 990,059 - +0.2 990,142 - pepN b0932 0.11 +0.5 990,147 + pepN b0932 0.12 +0.3 990,147 + pepN b0932 0.12 -0.6 990,201 + pepN b0932 0.14 -1.1 990,209 - pepN b0932 0.14 +0.3 990,209 - pepN b0932 0.14 -0.6 990,357 + pepN b0932 0.20 -0.1 990,357 + pepN b0932 0.20 -1.2 990,433 - pepN b0932 0.23 -0.8 990,433 - pepN b0932 0.23 -0.0 990,499 + pepN b0932 0.25 +0.1 990,554 + pepN b0932 0.27 +0.3 990,554 + pepN b0932 0.27 +0.6
Or see this region's nucleotide sequence