Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt speD and speE are separated by 15 nucleotides speE and yacC are separated by 105 nucleotides yacC and cueO are separated by 165 nucleotides
b0120: speD - S-adenosylmethionine decarboxylase proenzyme (NCBI), at 134,788 to 135,582
speD
b0121: speE - spermidine synthase (NCBI), at 135,598 to 136,464
speE
b0122: yacC - orf, hypothetical protein (VIMSS), at 136,570 to 136,917
yacC
b0123: cueO - multicopper oxidase (laccase) (NCBI), at 137,083 to 138,633
cueO
Position (kb) 136
137 Strain fitness (log2 ratio) -2
-1
0
1
2 at 135.643 kb on + strand at 135.705 kb on - strand, within speE at 135.749 kb on - strand, within speE at 135.848 kb on - strand, within speE at 135.851 kb on + strand, within speE at 135.851 kb on + strand, within speE at 135.934 kb on - strand, within speE at 135.934 kb on - strand, within speE at 135.994 kb on - strand, within speE at 136.088 kb on + strand, within speE at 136.096 kb on - strand, within speE at 136.148 kb on + strand, within speE at 136.189 kb on + strand, within speE at 136.191 kb on + strand, within speE at 136.257 kb on - strand, within speE at 136.402 kb on - strand at 136.402 kb on - strand at 136.424 kb on - strand at 136.516 kb on - strand at 136.516 kb on - strand at 136.733 kb on + strand, within yacC at 136.755 kb on - strand, within yacC at 136.779 kb on + strand, within yacC at 136.779 kb on + strand, within yacC at 136.859 kb on + strand, within yacC at 136.901 kb on - strand at 136.922 kb on + strand at 136.922 kb on + strand at 137.033 kb on + strand at 137.123 kb on + strand at 137.199 kb on + strand at 137.240 kb on + strand, within cueO at 137.240 kb on + strand, within cueO at 137.380 kb on - strand, within cueO at 137.401 kb on + strand, within cueO at 137.401 kb on - strand, within cueO at 137.401 kb on - strand, within cueO at 137.482 kb on - strand, within cueO at 137.485 kb on + strand, within cueO at 137.544 kb on - strand, within cueO at 137.544 kb on - strand, within cueO at 137.708 kb on + strand, within cueO at 137.708 kb on + strand, within cueO at 137.730 kb on - strand, within cueO at 137.730 kb on - strand, within cueO at 137.735 kb on - strand, within cueO at 137.852 kb on - strand, within cueO at 137.889 kb on + strand, within cueO at 137.897 kb on + strand, within cueO at 137.907 kb on + strand, within cueO at 137.913 kb on - strand, within cueO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM remove 135,643 + +1.0 135,705 - speE b0121 0.12 +1.3 135,749 - speE b0121 0.17 +0.4 135,848 - speE b0121 0.29 +0.2 135,851 + speE b0121 0.29 -0.1 135,851 + speE b0121 0.29 -1.4 135,934 - speE b0121 0.39 +0.5 135,934 - speE b0121 0.39 -1.9 135,994 - speE b0121 0.46 -0.2 136,088 + speE b0121 0.57 +0.9 136,096 - speE b0121 0.57 -0.5 136,148 + speE b0121 0.63 +0.1 136,189 + speE b0121 0.68 -0.7 136,191 + speE b0121 0.68 +0.7 136,257 - speE b0121 0.76 -1.4 136,402 - -0.3 136,402 - +0.3 136,424 - -2.0 136,516 - +0.9 136,516 - +1.0 136,733 + yacC b0122 0.47 -1.2 136,755 - yacC b0122 0.53 +0.5 136,779 + yacC b0122 0.60 -2.4 136,779 + yacC b0122 0.60 -0.7 136,859 + yacC b0122 0.83 +0.7 136,901 - +0.0 136,922 + -0.1 136,922 + -0.1 137,033 + +0.1 137,123 + -1.3 137,199 + -0.4 137,240 + cueO b0123 0.10 -1.2 137,240 + cueO b0123 0.10 +0.3 137,380 - cueO b0123 0.19 -0.3 137,401 + cueO b0123 0.21 +0.5 137,401 - cueO b0123 0.21 +0.1 137,401 - cueO b0123 0.21 +1.7 137,482 - cueO b0123 0.26 +0.8 137,485 + cueO b0123 0.26 +0.2 137,544 - cueO b0123 0.30 -0.0 137,544 - cueO b0123 0.30 +1.8 137,708 + cueO b0123 0.40 +0.1 137,708 + cueO b0123 0.40 +0.0 137,730 - cueO b0123 0.42 +1.3 137,730 - cueO b0123 0.42 +0.5 137,735 - cueO b0123 0.42 -0.1 137,852 - cueO b0123 0.50 -0.3 137,889 + cueO b0123 0.52 -0.5 137,897 + cueO b0123 0.52 -0.3 137,907 + cueO b0123 0.53 +0.0 137,913 - cueO b0123 0.54 +0.6
Or see this region's nucleotide sequence