Strain Fitness in Escherichia coli BW25113 around b0047

Experiment: LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyaaU and kefF are separated by 107 nucleotideskefF and kefC overlap by 8 nucleotideskefC and folA are separated by 191 nucleotidesfolA and apaH are separated by 77 nucleotides b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138 yaaU b0046: kefF - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 47,246 to 47,776 kefF b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631 kefC b0048: folA - dihydrofolate reductase (NCBI), at 49,823 to 50,302 folA b0049: apaH - diadenosinetetraphosphatase (NCBI), at 50,380 to 51,222 apaH Position (kb) 47 48 49 50Strain fitness (log2 ratio) -2 -1 0 1 2 3at 46.800 kb on + strand, within yaaUat 46.920 kb on - strand, within yaaUat 47.003 kb on + strand, within yaaUat 47.067 kb on + strandat 47.097 kb on - strandat 47.097 kb on - strandat 47.113 kb on - strandat 47.113 kb on - strandat 47.202 kb on - strandat 47.225 kb on + strandat 47.225 kb on + strandat 47.247 kb on - strandat 47.269 kb on + strandat 47.359 kb on - strand, within kefFat 47.364 kb on - strand, within kefFat 47.364 kb on - strand, within kefFat 47.364 kb on - strand, within kefFat 47.364 kb on - strand, within kefFat 47.402 kb on - strand, within kefFat 47.412 kb on - strand, within kefFat 47.446 kb on + strand, within kefFat 47.454 kb on + strand, within kefFat 47.503 kb on + strand, within kefFat 47.504 kb on + strand, within kefFat 47.504 kb on + strand, within kefFat 47.639 kb on + strand, within kefFat 47.651 kb on + strand, within kefFat 47.675 kb on + strand, within kefFat 47.681 kb on + strand, within kefFat 47.681 kb on + strand, within kefFat 47.730 kb on + strandat 47.762 kb on + strandat 47.774 kb on - strandat 47.784 kb on - strandat 47.823 kb on + strandat 47.823 kb on + strandat 47.832 kb on + strandat 47.832 kb on + strandat 47.836 kb on + strandat 47.848 kb on + strandat 47.859 kb on + strandat 47.921 kb on + strandat 47.948 kb on + strandat 47.948 kb on + strandat 47.958 kb on + strand, within kefCat 47.958 kb on + strand, within kefCat 47.977 kb on - strand, within kefCat 48.009 kb on - strand, within kefCat 48.009 kb on - strand, within kefCat 48.009 kb on - strand, within kefCat 48.031 kb on + strand, within kefCat 48.227 kb on + strand, within kefCat 48.227 kb on - strand, within kefCat 48.228 kb on + strand, within kefCat 48.235 kb on - strand, within kefCat 48.253 kb on - strand, within kefCat 48.348 kb on + strand, within kefCat 48.394 kb on + strand, within kefCat 48.408 kb on + strand, within kefCat 48.408 kb on + strand, within kefCat 48.569 kb on + strand, within kefCat 48.569 kb on + strand, within kefCat 48.614 kb on + strand, within kefCat 48.760 kb on + strand, within kefCat 48.760 kb on + strand, within kefCat 48.786 kb on - strand, within kefCat 48.907 kb on + strand, within kefCat 48.958 kb on + strand, within kefCat 48.968 kb on - strand, within kefCat 49.068 kb on - strand, within kefCat 49.068 kb on - strand, within kefCat 49.149 kb on - strand, within kefCat 49.149 kb on - strand, within kefCat 49.202 kb on + strand, within kefCat 49.281 kb on + strand, within kefCat 49.289 kb on - strand, within kefCat 49.289 kb on - strand, within kefCat 49.334 kb on + strand, within kefCat 49.334 kb on + strand, within kefCat 49.356 kb on - strand, within kefCat 49.356 kb on - strand, within kefCat 49.369 kb on + strand, within kefCat 49.369 kb on + strand, within kefCat 49.409 kb on - strand, within kefCat 49.431 kb on + strand, within kefCat 49.487 kb on - strandat 49.493 kb on - strandat 49.709 kb on + strandat 50.361 kb on + strandat 50.361 kb on + strandat 50.370 kb on - strandat 50.376 kb on + strandat 50.461 kb on + strandat 50.469 kb on - strand, within apaHat 50.501 kb on + strand, within apaHat 50.507 kb on - strand, within apaHat 50.568 kb on - strand, within apaH

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with 2-Mercaptopyridine N-oxide 0.0000078125 mM
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46,800 + yaaU b0045 0.75 +1.3
46,920 - yaaU b0045 0.84 +0.5
47,003 + yaaU b0045 0.90 +0.4
47,067 + +0.7
47,097 - +0.5
47,097 - -2.1
47,113 - +0.5
47,113 - +1.3
47,202 - +2.3
47,225 + -0.1
47,225 + +0.3
47,247 - -1.5
47,269 + +0.5
47,359 - kefF b0046 0.21 +0.2
47,364 - kefF b0046 0.22 -0.5
47,364 - kefF b0046 0.22 -0.6
47,364 - kefF b0046 0.22 +0.2
47,364 - kefF b0046 0.22 +3.0
47,402 - kefF b0046 0.29 -1.1
47,412 - kefF b0046 0.31 +0.3
47,446 + kefF b0046 0.38 -0.7
47,454 + kefF b0046 0.39 +1.0
47,503 + kefF b0046 0.48 -0.7
47,504 + kefF b0046 0.49 -0.2
47,504 + kefF b0046 0.49 -1.1
47,639 + kefF b0046 0.74 -0.1
47,651 + kefF b0046 0.76 +0.2
47,675 + kefF b0046 0.81 +0.1
47,681 + kefF b0046 0.82 -0.6
47,681 + kefF b0046 0.82 -1.5
47,730 + -0.9
47,762 + -0.8
47,774 - +0.8
47,784 - +1.8
47,823 + +0.7
47,823 + +0.3
47,832 + -2.1
47,832 + +0.3
47,836 + +0.5
47,848 + -0.2
47,859 + +0.0
47,921 + -0.5
47,948 + -1.1
47,948 + -1.5
47,958 + kefC b0047 0.10 -0.5
47,958 + kefC b0047 0.10 +0.3
47,977 - kefC b0047 0.11 -0.5
48,009 - kefC b0047 0.13 +0.3
48,009 - kefC b0047 0.13 +1.6
48,009 - kefC b0047 0.13 +1.8
48,031 + kefC b0047 0.14 +0.4
48,227 + kefC b0047 0.25 -0.6
48,227 - kefC b0047 0.25 +1.1
48,228 + kefC b0047 0.25 -0.3
48,235 - kefC b0047 0.25 -0.1
48,253 - kefC b0047 0.26 -1.1
48,348 + kefC b0047 0.31 -0.6
48,394 + kefC b0047 0.34 -0.1
48,408 + kefC b0047 0.34 +0.1
48,408 + kefC b0047 0.34 +0.1
48,569 + kefC b0047 0.43 -0.3
48,569 + kefC b0047 0.43 -0.1
48,614 + kefC b0047 0.45 +1.3
48,760 + kefC b0047 0.53 -1.3
48,760 + kefC b0047 0.53 -0.5
48,786 - kefC b0047 0.55 -0.5
48,907 + kefC b0047 0.61 +0.3
48,958 + kefC b0047 0.64 -0.6
48,968 - kefC b0047 0.64 +1.3
49,068 - kefC b0047 0.70 -1.1
49,068 - kefC b0047 0.70 -0.5
49,149 - kefC b0047 0.74 +1.1
49,149 - kefC b0047 0.74 -0.3
49,202 + kefC b0047 0.77 +0.6
49,281 + kefC b0047 0.81 -0.2
49,289 - kefC b0047 0.82 +0.3
49,289 - kefC b0047 0.82 +0.0
49,334 + kefC b0047 0.84 +2.6
49,334 + kefC b0047 0.84 +0.1
49,356 - kefC b0047 0.85 +1.4
49,356 - kefC b0047 0.85 -0.7
49,369 + kefC b0047 0.86 -0.2
49,369 + kefC b0047 0.86 -0.6
49,409 - kefC b0047 0.88 +0.3
49,431 + kefC b0047 0.89 -1.2
49,487 - +0.3
49,493 - +0.1
49,709 + +1.3
50,361 + -0.9
50,361 + -0.5
50,370 - +0.3
50,376 + -0.8
50,461 + +1.3
50,469 - apaH b0049 0.11 -1.1
50,501 + apaH b0049 0.14 +2.8
50,507 - apaH b0049 0.15 -0.3
50,568 - apaH b0049 0.22 +1.3

Or see this region's nucleotide sequence