Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phnF and phnE are separated by 20 nucleotides phnE and phnD are separated by 521 nucleotides phnD and phnC are separated by 24 nucleotides phnC and phnB are separated by 132 nucleotides
b4102: phnF - predicted DNA-binding transcriptional regulator of phosphonate uptake and biodegradation (NCBI), at 4,319,720 to 4,320,445
phnF
b4104: phnE - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,320,466 to 4,320,837
phnE
b4105: phnD - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,321,359 to 4,322,375
phnD
b4106: phnC - phosphonate/organophosphate ester transporter subunit (NCBI), at 4,322,400 to 4,323,188
phnC
b4107: phnB - hypothetical protein (NCBI), at 4,323,321 to 4,323,764
phnB
Position (kb)
4321
4322
4323 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4320.405 kb on - strand at 4320.412 kb on + strand at 4320.412 kb on + strand at 4320.652 kb on - strand, within phnE at 4320.670 kb on - strand, within phnE at 4320.678 kb on - strand, within phnE at 4320.691 kb on + strand, within phnE at 4320.804 kb on + strand at 4320.842 kb on + strand at 4320.937 kb on + strand at 4321.008 kb on - strand at 4321.144 kb on - strand at 4321.193 kb on - strand at 4321.193 kb on - strand at 4321.193 kb on - strand at 4321.208 kb on - strand at 4321.248 kb on - strand at 4321.254 kb on + strand at 4321.308 kb on + strand at 4321.310 kb on + strand at 4321.310 kb on + strand at 4321.316 kb on - strand at 4321.421 kb on - strand at 4321.440 kb on - strand at 4321.440 kb on - strand at 4321.512 kb on - strand, within phnD at 4321.572 kb on + strand, within phnD at 4321.637 kb on - strand, within phnD at 4321.637 kb on - strand, within phnD at 4321.713 kb on - strand, within phnD at 4321.713 kb on - strand, within phnD at 4321.716 kb on + strand, within phnD at 4321.834 kb on + strand, within phnD at 4321.834 kb on + strand, within phnD at 4321.915 kb on - strand, within phnD at 4322.004 kb on + strand, within phnD at 4322.088 kb on - strand, within phnD at 4322.155 kb on + strand, within phnD at 4322.155 kb on + strand, within phnD at 4322.249 kb on - strand, within phnD at 4322.310 kb on + strand at 4322.328 kb on + strand at 4322.335 kb on - strand at 4322.415 kb on + strand at 4322.415 kb on + strand at 4322.429 kb on + strand at 4322.463 kb on + strand at 4322.471 kb on + strand at 4322.471 kb on + strand at 4322.525 kb on + strand, within phnC at 4322.538 kb on - strand, within phnC at 4322.563 kb on + strand, within phnC at 4322.563 kb on + strand, within phnC at 4322.841 kb on - strand, within phnC at 4322.856 kb on - strand, within phnC at 4322.915 kb on + strand, within phnC at 4322.915 kb on + strand, within phnC at 4322.963 kb on - strand, within phnC at 4322.988 kb on - strand, within phnC at 4323.255 kb on + strand at 4323.352 kb on + strand at 4323.369 kb on + strand, within phnB at 4323.369 kb on + strand, within phnB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 4,320,405 - -0.5 4,320,412 + -0.4 4,320,412 + -0.8 4,320,652 - phnE b4104 0.50 -2.4 4,320,670 - phnE b4104 0.55 +0.4 4,320,678 - phnE b4104 0.57 +0.6 4,320,691 + phnE b4104 0.60 -0.3 4,320,804 + -0.8 4,320,842 + +1.1 4,320,937 + +0.7 4,321,008 - -2.2 4,321,144 - +0.1 4,321,193 - -0.7 4,321,193 - +0.8 4,321,193 - +0.5 4,321,208 - +0.2 4,321,248 - +1.1 4,321,254 + +0.2 4,321,308 + -0.9 4,321,310 + -1.5 4,321,310 + +1.2 4,321,316 - -0.2 4,321,421 - +0.2 4,321,440 - +0.4 4,321,440 - +1.4 4,321,512 - phnD b4105 0.15 -0.0 4,321,572 + phnD b4105 0.21 -0.6 4,321,637 - phnD b4105 0.27 -1.0 4,321,637 - phnD b4105 0.27 -1.9 4,321,713 - phnD b4105 0.35 +0.6 4,321,713 - phnD b4105 0.35 +1.6 4,321,716 + phnD b4105 0.35 -0.0 4,321,834 + phnD b4105 0.47 -1.2 4,321,834 + phnD b4105 0.47 -0.0 4,321,915 - phnD b4105 0.55 +0.5 4,322,004 + phnD b4105 0.63 +0.1 4,322,088 - phnD b4105 0.72 -1.3 4,322,155 + phnD b4105 0.78 +0.5 4,322,155 + phnD b4105 0.78 +0.2 4,322,249 - phnD b4105 0.88 -0.4 4,322,310 + +1.8 4,322,328 + +0.2 4,322,335 - -0.7 4,322,415 + -1.7 4,322,415 + +1.2 4,322,429 + +0.2 4,322,463 + -0.1 4,322,471 + -2.0 4,322,471 + +1.4 4,322,525 + phnC b4106 0.16 +0.0 4,322,538 - phnC b4106 0.17 +0.5 4,322,563 + phnC b4106 0.21 +0.3 4,322,563 + phnC b4106 0.21 +1.5 4,322,841 - phnC b4106 0.56 +0.3 4,322,856 - phnC b4106 0.58 +0.8 4,322,915 + phnC b4106 0.65 +1.0 4,322,915 + phnC b4106 0.65 +0.6 4,322,963 - phnC b4106 0.71 -0.8 4,322,988 - phnC b4106 0.75 +0.2 4,323,255 + +0.4 4,323,352 + -2.1 4,323,369 + phnB b4107 0.11 +0.0 4,323,369 + phnB b4107 0.11 -0.0
Or see this region's nucleotide sequence