Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pcm and surE overlap by 7 nucleotides surE and truD overlap by 20 nucleotides truD and ispF overlap by 4 nucleotides ispF and ispD overlap by 1 nucleotides
b2743: pcm - protein-L-isoaspartate O-methyltransferase (NCBI), at 2,866,915 to 2,867,541
pcm
b2744: surE - acid phosphatase (NCBI), at 2,867,535 to 2,868,296
surE
b2745: truD - tRNA pseudouridine synthase D (NCBI), at 2,868,277 to 2,869,326
truD
b2746: ispF - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (NCBI), at 2,869,323 to 2,869,802
ispF
b2747: ispD - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (NCBI), at 2,869,802 to 2,870,512
ispD
Position (kb)
2868
2869
2870 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.354 kb on + strand, within pcm at 2867.359 kb on - strand, within pcm at 2867.541 kb on - strand at 2867.541 kb on - strand at 2867.616 kb on - strand, within surE at 2867.623 kb on + strand, within surE at 2867.631 kb on + strand, within surE at 2867.672 kb on - strand, within surE at 2867.701 kb on + strand, within surE at 2867.709 kb on - strand, within surE at 2867.747 kb on + strand, within surE at 2867.747 kb on + strand, within surE at 2867.862 kb on - strand, within surE at 2867.862 kb on - strand, within surE at 2867.862 kb on - strand, within surE at 2867.883 kb on + strand, within surE at 2867.883 kb on + strand, within surE at 2868.038 kb on - strand, within surE at 2868.043 kb on - strand, within surE at 2868.043 kb on - strand, within surE at 2868.152 kb on + strand, within surE at 2868.266 kb on - strand at 2868.284 kb on + strand at 2868.332 kb on - strand at 2868.421 kb on - strand, within truD at 2868.647 kb on + strand, within truD at 2868.647 kb on - strand, within truD at 2868.744 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.779 kb on + strand, within truD at 2868.785 kb on - strand, within truD at 2868.785 kb on - strand, within truD at 2868.791 kb on + strand, within truD at 2868.845 kb on - strand, within truD at 2868.845 kb on - strand, within truD at 2868.930 kb on + strand, within truD at 2868.930 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.986 kb on + strand, within truD at 2868.996 kb on - strand, within truD at 2869.037 kb on + strand, within truD at 2869.118 kb on - strand, within truD at 2869.118 kb on - strand, within truD at 2869.194 kb on + strand, within truD at 2869.194 kb on + strand, within truD at 2869.292 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 2,867,354 + pcm b2743 0.70 -0.2 2,867,354 + pcm b2743 0.70 -0.4 2,867,354 + pcm b2743 0.70 -2.3 2,867,354 + pcm b2743 0.70 -0.1 2,867,354 + pcm b2743 0.70 -1.6 2,867,359 - pcm b2743 0.71 -0.8 2,867,541 - -0.6 2,867,541 - +0.3 2,867,616 - surE b2744 0.11 -1.1 2,867,623 + surE b2744 0.12 -0.5 2,867,631 + surE b2744 0.13 -2.3 2,867,672 - surE b2744 0.18 -0.4 2,867,701 + surE b2744 0.22 +0.9 2,867,709 - surE b2744 0.23 -2.6 2,867,747 + surE b2744 0.28 -0.2 2,867,747 + surE b2744 0.28 -2.1 2,867,862 - surE b2744 0.43 -2.0 2,867,862 - surE b2744 0.43 -0.1 2,867,862 - surE b2744 0.43 -1.0 2,867,883 + surE b2744 0.46 +0.6 2,867,883 + surE b2744 0.46 -0.8 2,868,038 - surE b2744 0.66 -3.0 2,868,043 - surE b2744 0.67 +0.0 2,868,043 - surE b2744 0.67 -0.2 2,868,152 + surE b2744 0.81 +1.5 2,868,266 - -2.2 2,868,284 + +2.4 2,868,332 - +0.3 2,868,421 - truD b2745 0.14 -0.5 2,868,647 + truD b2745 0.35 -0.9 2,868,647 - truD b2745 0.35 +0.1 2,868,744 + truD b2745 0.44 +0.4 2,868,779 + truD b2745 0.48 +0.2 2,868,779 + truD b2745 0.48 +0.3 2,868,785 - truD b2745 0.48 -2.8 2,868,785 - truD b2745 0.48 +1.0 2,868,791 + truD b2745 0.49 -0.7 2,868,845 - truD b2745 0.54 -2.0 2,868,845 - truD b2745 0.54 -0.7 2,868,930 + truD b2745 0.62 -1.6 2,868,930 + truD b2745 0.62 -0.4 2,868,986 + truD b2745 0.68 +0.6 2,868,986 + truD b2745 0.68 -1.8 2,868,996 - truD b2745 0.68 -0.2 2,869,037 + truD b2745 0.72 +1.2 2,869,118 - truD b2745 0.80 +1.2 2,869,118 - truD b2745 0.80 +1.5 2,869,194 + truD b2745 0.87 +0.9 2,869,194 + truD b2745 0.87 -0.4 2,869,292 - -1.4
Or see this region's nucleotide sequence