Strain Fitness in Escherichia coli BW25113 around b2464

Experiment: LB Aerobic with Irgasan 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmaeB and talA are separated by 288 nucleotidestalA and tktB are separated by 19 nucleotides b2463: maeB - malic enzyme (NCBI), at 2,574,120 to 2,576,399 maeB b2464: talA - transaldolase A (NCBI), at 2,576,688 to 2,577,638 talA b2465: tktB - transketolase 2, thiamin-binding (NCBI), at 2,577,658 to 2,579,661 tktB Position (kb) 2576 2577 2578Strain fitness (log2 ratio) -2 -1 0 1 2at 2575.869 kb on + strand, within maeBat 2576.054 kb on + strand, within maeBat 2576.062 kb on - strand, within maeBat 2576.062 kb on - strand, within maeBat 2576.164 kb on + strand, within maeBat 2576.174 kb on - strandat 2576.281 kb on - strandat 2576.376 kb on + strandat 2576.391 kb on - strandat 2576.391 kb on - strandat 2576.462 kb on + strandat 2576.472 kb on + strandat 2576.480 kb on - strandat 2576.480 kb on - strandat 2576.586 kb on - strandat 2576.853 kb on - strand, within talAat 2576.984 kb on - strand, within talAat 2577.042 kb on - strand, within talAat 2577.044 kb on + strand, within talAat 2577.044 kb on + strand, within talAat 2577.060 kb on - strand, within talAat 2577.060 kb on - strand, within talAat 2577.263 kb on + strand, within talAat 2577.266 kb on + strand, within talAat 2577.491 kb on - strand, within talAat 2577.659 kb on - strandat 2577.696 kb on + strandat 2577.704 kb on - strandat 2577.706 kb on - strandat 2577.706 kb on - strandat 2577.856 kb on - strandat 2577.856 kb on - strandat 2578.022 kb on + strand, within tktBat 2578.083 kb on - strand, within tktBat 2578.083 kb on - strand, within tktBat 2578.169 kb on + strand, within tktBat 2578.352 kb on + strand, within tktBat 2578.372 kb on + strand, within tktBat 2578.439 kb on + strand, within tktBat 2578.439 kb on + strand, within tktBat 2578.624 kb on + strand, within tktB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM
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2,575,869 + maeB b2463 0.77 -0.6
2,576,054 + maeB b2463 0.85 -0.5
2,576,062 - maeB b2463 0.85 +0.9
2,576,062 - maeB b2463 0.85 +0.5
2,576,164 + maeB b2463 0.90 +0.3
2,576,174 - -0.7
2,576,281 - -0.5
2,576,376 + -0.9
2,576,391 - -1.0
2,576,391 - +1.3
2,576,462 + +0.7
2,576,472 + -1.0
2,576,480 - +0.2
2,576,480 - -1.2
2,576,586 - -0.1
2,576,853 - talA b2464 0.17 -0.8
2,576,984 - talA b2464 0.31 -0.6
2,577,042 - talA b2464 0.37 +1.1
2,577,044 + talA b2464 0.37 +0.4
2,577,044 + talA b2464 0.37 +0.1
2,577,060 - talA b2464 0.39 -1.0
2,577,060 - talA b2464 0.39 -0.6
2,577,263 + talA b2464 0.60 -0.8
2,577,266 + talA b2464 0.61 -1.7
2,577,491 - talA b2464 0.84 -0.0
2,577,659 - +1.1
2,577,696 + -1.7
2,577,704 - -0.6
2,577,706 - -0.4
2,577,706 - -0.8
2,577,856 - +0.6
2,577,856 - -1.2
2,578,022 + tktB b2465 0.18 +0.5
2,578,083 - tktB b2465 0.21 -1.6
2,578,083 - tktB b2465 0.21 +2.0
2,578,169 + tktB b2465 0.25 -1.6
2,578,352 + tktB b2465 0.35 -2.2
2,578,372 + tktB b2465 0.36 -1.2
2,578,439 + tktB b2465 0.39 -0.5
2,578,439 + tktB b2465 0.39 -0.9
2,578,624 + tktB b2465 0.48 +1.0

Or see this region's nucleotide sequence