Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt accD and dedA are separated by 155 nucleotides dedA and truA are separated by 82 nucleotides truA and usg overlap by 1 nucleotides
b2316: accD - acetyl-CoA carboxylase subunit beta (NCBI), at 2,431,034 to 2,431,948
accD
b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763
dedA
b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658
truA
b2319: usg - hypothetical protein (NCBI), at 2,433,658 to 2,434,671
usg
Position (kb)
2432
2433
2434 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2432.025 kb on - strand at 2432.025 kb on - strand at 2432.196 kb on + strand, within dedA at 2432.269 kb on - strand, within dedA at 2432.269 kb on - strand, within dedA at 2432.290 kb on - strand, within dedA at 2432.290 kb on - strand, within dedA at 2432.494 kb on + strand, within dedA at 2432.650 kb on + strand, within dedA at 2432.650 kb on + strand, within dedA at 2432.800 kb on + strand at 2432.808 kb on - strand at 2432.808 kb on - strand at 2433.004 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.141 kb on + strand, within truA at 2433.157 kb on + strand, within truA at 2433.160 kb on - strand, within truA at 2433.160 kb on - strand, within truA at 2433.170 kb on - strand, within truA at 2433.363 kb on - strand at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.465 kb on - strand, within truA at 2433.600 kb on + strand at 2433.600 kb on + strand at 2433.656 kb on + strand at 2433.664 kb on - strand at 2433.670 kb on + strand at 2433.670 kb on + strand at 2433.678 kb on - strand at 2433.753 kb on - strand at 2433.846 kb on + strand, within usg at 2433.853 kb on - strand, within usg at 2433.853 kb on - strand, within usg at 2433.910 kb on + strand, within usg at 2433.943 kb on + strand, within usg at 2433.952 kb on - strand, within usg at 2433.952 kb on - strand, within usg at 2434.057 kb on + strand, within usg at 2434.167 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.287 kb on - strand, within usg at 2434.339 kb on + strand, within usg at 2434.360 kb on + strand, within usg at 2434.626 kb on - strand at 2434.647 kb on + strand at 2434.647 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 2,432,025 - +0.9 2,432,025 - +1.6 2,432,196 + dedA b2317 0.14 -2.1 2,432,269 - dedA b2317 0.25 -2.2 2,432,269 - dedA b2317 0.25 -1.3 2,432,290 - dedA b2317 0.28 -1.6 2,432,290 - dedA b2317 0.28 -2.4 2,432,494 + dedA b2317 0.59 -1.3 2,432,650 + dedA b2317 0.83 -1.2 2,432,650 + dedA b2317 0.83 +1.4 2,432,800 + +0.1 2,432,808 - +1.1 2,432,808 - -1.7 2,433,004 + truA b2318 0.19 -1.9 2,433,141 + truA b2318 0.36 -1.1 2,433,141 + truA b2318 0.36 -2.1 2,433,157 + truA b2318 0.38 -0.1 2,433,160 - truA b2318 0.39 -0.4 2,433,160 - truA b2318 0.39 +0.9 2,433,170 - truA b2318 0.40 +0.3 2,433,363 - +0.9 2,433,465 - truA b2318 0.76 +2.9 2,433,465 - truA b2318 0.76 -0.1 2,433,465 - truA b2318 0.76 -0.1 2,433,465 - truA b2318 0.76 +0.9 2,433,600 + +0.9 2,433,600 + -0.7 2,433,656 + +0.9 2,433,664 - -0.6 2,433,670 + +0.1 2,433,670 + -0.7 2,433,678 - +0.0 2,433,753 - +0.8 2,433,846 + usg b2319 0.19 -0.1 2,433,853 - usg b2319 0.19 +0.4 2,433,853 - usg b2319 0.19 -0.7 2,433,910 + usg b2319 0.25 -1.7 2,433,943 + usg b2319 0.28 -1.4 2,433,952 - usg b2319 0.29 -0.4 2,433,952 - usg b2319 0.29 +0.1 2,434,057 + usg b2319 0.39 -1.4 2,434,167 - usg b2319 0.50 -1.2 2,434,287 - usg b2319 0.62 +0.3 2,434,287 - usg b2319 0.62 -0.1 2,434,339 + usg b2319 0.67 +1.3 2,434,360 + usg b2319 0.69 -1.3 2,434,626 - -2.8 2,434,647 + -0.4 2,434,647 + -1.1
Or see this region's nucleotide sequence