Strain Fitness in Escherichia coli BW25113 around b2253

Experiment: LB Aerobic with Irgasan 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyfaO and ais are separated by 38 nucleotidesais and arnB are separated by 307 nucleotidesarnB and yfbF are separated by 3 nucleotidesyfbF and yfbG overlap by 1 nucleotides b2251: yfaO - predicted NUDIX hydrolase (NCBI), at 2,362,576 to 2,363,001 yfaO b2252: ais - hypothetical protein (NCBI), at 2,363,040 to 2,363,642 ais b2253: arnB - uridine 5'-(beta-1-threo-pentapyranosyl-4-ulose diphosphate) aminotransferase, PLP-dependent (RefSeq), at 2,363,950 to 2,365,089 arnB b2254: yfbF - undecaprenyl phosphate-L-Ara4FN transferase (NCBI), at 2,365,093 to 2,366,061 yfbF b2255: yfbG - hypothetical protein (NCBI), at 2,366,061 to 2,368,043 yfbG Position (kb) 2363 2364 2365 2366Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 2362.994 kb on + strandat 2363.002 kb on + strandat 2363.002 kb on + strandat 2363.003 kb on + strandat 2363.008 kb on + strandat 2363.008 kb on + strandat 2363.082 kb on + strandat 2363.082 kb on + strandat 2363.084 kb on + strandat 2363.088 kb on + strandat 2363.093 kb on + strandat 2363.093 kb on + strandat 2363.126 kb on - strand, within aisat 2363.142 kb on - strand, within aisat 2363.153 kb on + strand, within aisat 2363.244 kb on + strand, within aisat 2363.244 kb on + strand, within aisat 2363.275 kb on + strand, within aisat 2363.275 kb on + strand, within aisat 2363.299 kb on + strand, within aisat 2363.320 kb on + strand, within aisat 2363.328 kb on - strand, within aisat 2363.373 kb on - strand, within aisat 2363.375 kb on + strand, within aisat 2363.375 kb on + strand, within aisat 2363.375 kb on + strand, within aisat 2363.383 kb on - strand, within aisat 2363.385 kb on + strand, within aisat 2363.387 kb on + strand, within aisat 2363.387 kb on + strand, within aisat 2363.387 kb on + strand, within aisat 2363.387 kb on + strand, within aisat 2363.393 kb on - strand, within aisat 2363.395 kb on - strand, within aisat 2363.404 kb on - strand, within aisat 2363.418 kb on + strand, within aisat 2363.418 kb on + strand, within aisat 2363.426 kb on + strand, within aisat 2363.427 kb on + strand, within aisat 2363.430 kb on - strand, within aisat 2363.434 kb on - strand, within aisat 2363.445 kb on - strand, within aisat 2363.451 kb on + strand, within aisat 2363.468 kb on + strand, within aisat 2363.468 kb on + strand, within aisat 2363.492 kb on + strand, within aisat 2363.500 kb on - strand, within aisat 2363.505 kb on - strand, within aisat 2363.505 kb on - strand, within aisat 2363.507 kb on + strand, within aisat 2363.507 kb on + strand, within aisat 2363.510 kb on - strand, within aisat 2363.525 kb on + strand, within aisat 2363.533 kb on + strand, within aisat 2363.533 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.536 kb on + strand, within aisat 2363.543 kb on - strand, within aisat 2363.544 kb on - strand, within aisat 2363.544 kb on - strand, within aisat 2363.544 kb on - strand, within aisat 2363.549 kb on - strand, within aisat 2363.554 kb on + strand, within aisat 2363.562 kb on - strand, within aisat 2363.645 kb on + strandat 2363.669 kb on + strandat 2363.699 kb on - strandat 2363.855 kb on - strandat 2364.000 kb on + strandat 2364.013 kb on - strandat 2364.016 kb on + strandat 2364.026 kb on - strandat 2364.036 kb on - strandat 2364.041 kb on - strandat 2364.041 kb on - strandat 2364.168 kb on - strand, within arnBat 2364.168 kb on - strand, within arnBat 2364.185 kb on - strand, within arnBat 2364.192 kb on + strand, within arnBat 2364.195 kb on - strand, within arnBat 2364.244 kb on - strand, within arnBat 2364.301 kb on + strand, within arnBat 2364.314 kb on + strand, within arnBat 2364.333 kb on + strand, within arnBat 2364.514 kb on - strand, within arnBat 2364.522 kb on + strand, within arnBat 2364.590 kb on - strand, within arnBat 2364.590 kb on - strand, within arnBat 2364.751 kb on - strand, within arnBat 2364.796 kb on - strand, within arnBat 2364.796 kb on - strand, within arnBat 2364.815 kb on - strand, within arnBat 2364.844 kb on + strand, within arnBat 2364.844 kb on + strand, within arnBat 2364.857 kb on - strand, within arnBat 2364.863 kb on + strand, within arnBat 2364.863 kb on + strand, within arnBat 2364.918 kb on - strand, within arnBat 2364.926 kb on + strand, within arnBat 2364.926 kb on + strand, within arnBat 2365.002 kb on + strandat 2365.021 kb on + strandat 2365.035 kb on - strandat 2365.053 kb on + strandat 2365.053 kb on + strandat 2365.056 kb on + strandat 2365.179 kb on - strandat 2365.181 kb on + strandat 2365.198 kb on - strand, within yfbFat 2365.200 kb on + strand, within yfbFat 2365.208 kb on - strand, within yfbFat 2365.220 kb on - strand, within yfbFat 2365.264 kb on - strand, within yfbFat 2365.290 kb on - strand, within yfbFat 2365.290 kb on - strand, within yfbFat 2365.414 kb on + strand, within yfbFat 2365.433 kb on + strand, within yfbFat 2365.450 kb on + strand, within yfbFat 2365.477 kb on - strand, within yfbFat 2365.529 kb on + strand, within yfbFat 2365.555 kb on + strand, within yfbFat 2365.555 kb on + strand, within yfbFat 2365.637 kb on + strand, within yfbFat 2365.773 kb on + strand, within yfbFat 2365.786 kb on - strand, within yfbFat 2365.786 kb on - strand, within yfbFat 2365.804 kb on - strand, within yfbFat 2365.854 kb on - strand, within yfbFat 2365.866 kb on + strand, within yfbFat 2365.866 kb on + strand, within yfbFat 2365.965 kb on - strandat 2365.983 kb on + strandat 2365.984 kb on + strandat 2365.984 kb on + strandat 2366.037 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM
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2,362,994 + -0.7
2,363,002 + +0.8
2,363,002 + -0.2
2,363,003 + -0.6
2,363,008 + -0.2
2,363,008 + -1.2
2,363,082 + -2.0
2,363,082 + +2.4
2,363,084 + +0.8
2,363,088 + +0.8
2,363,093 + +0.8
2,363,093 + -0.2
2,363,126 - ais b2252 0.14 +4.2
2,363,142 - ais b2252 0.17 -1.2
2,363,153 + ais b2252 0.19 +0.8
2,363,244 + ais b2252 0.34 +1.4
2,363,244 + ais b2252 0.34 -0.7
2,363,275 + ais b2252 0.39 +0.3
2,363,275 + ais b2252 0.39 +0.8
2,363,299 + ais b2252 0.43 -0.2
2,363,320 + ais b2252 0.46 +1.4
2,363,328 - ais b2252 0.48 +0.8
2,363,373 - ais b2252 0.55 +2.6
2,363,375 + ais b2252 0.56 +0.8
2,363,375 + ais b2252 0.56 -0.2
2,363,375 + ais b2252 0.56 +0.8
2,363,383 - ais b2252 0.57 +0.8
2,363,385 + ais b2252 0.57 +0.8
2,363,387 + ais b2252 0.58 +0.8
2,363,387 + ais b2252 0.58 +0.8
2,363,387 + ais b2252 0.58 -0.2
2,363,387 + ais b2252 0.58 -0.2
2,363,393 - ais b2252 0.59 +0.8
2,363,395 - ais b2252 0.59 +0.8
2,363,404 - ais b2252 0.60 -0.2
2,363,418 + ais b2252 0.63 -0.7
2,363,418 + ais b2252 0.63 -0.7
2,363,426 + ais b2252 0.64 +0.8
2,363,427 + ais b2252 0.64 -0.7
2,363,430 - ais b2252 0.65 +3.4
2,363,434 - ais b2252 0.65 -1.2
2,363,445 - ais b2252 0.67 +0.8
2,363,451 + ais b2252 0.68 +0.8
2,363,468 + ais b2252 0.71 -0.7
2,363,468 + ais b2252 0.71 -0.2
2,363,492 + ais b2252 0.75 +1.8
2,363,500 - ais b2252 0.76 +0.8
2,363,505 - ais b2252 0.77 +0.8
2,363,505 - ais b2252 0.77 +0.8
2,363,507 + ais b2252 0.77 -0.2
2,363,507 + ais b2252 0.77 -0.2
2,363,510 - ais b2252 0.78 -0.2
2,363,525 + ais b2252 0.80 +0.8
2,363,533 + ais b2252 0.82 +0.8
2,363,533 + ais b2252 0.82 +0.8
2,363,536 + ais b2252 0.82 +0.8
2,363,536 + ais b2252 0.82 -0.2
2,363,536 + ais b2252 0.82 -0.2
2,363,536 + ais b2252 0.82 +0.8
2,363,536 + ais b2252 0.82 -0.2
2,363,536 + ais b2252 0.82 +0.8
2,363,543 - ais b2252 0.83 -0.7
2,363,544 - ais b2252 0.84 -0.2
2,363,544 - ais b2252 0.84 +0.8
2,363,544 - ais b2252 0.84 +0.8
2,363,549 - ais b2252 0.84 +0.8
2,363,554 + ais b2252 0.85 -0.2
2,363,562 - ais b2252 0.87 +0.8
2,363,645 + +0.8
2,363,669 + +0.8
2,363,699 - +0.8
2,363,855 - +0.8
2,364,000 + +0.8
2,364,013 - +0.5
2,364,016 + -0.2
2,364,026 - +0.4
2,364,036 - +0.3
2,364,041 - +1.3
2,364,041 - +0.5
2,364,168 - arnB b2253 0.19 +0.7
2,364,168 - arnB b2253 0.19 +1.4
2,364,185 - arnB b2253 0.21 -0.6
2,364,192 + arnB b2253 0.21 +3.7
2,364,195 - arnB b2253 0.21 -0.2
2,364,244 - arnB b2253 0.26 +0.8
2,364,301 + arnB b2253 0.31 +0.3
2,364,314 + arnB b2253 0.32 +0.4
2,364,333 + arnB b2253 0.34 +0.3
2,364,514 - arnB b2253 0.49 +0.3
2,364,522 + arnB b2253 0.50 -0.7
2,364,590 - arnB b2253 0.56 +2.1
2,364,590 - arnB b2253 0.56 -0.2
2,364,751 - arnB b2253 0.70 +1.3
2,364,796 - arnB b2253 0.74 +0.4
2,364,796 - arnB b2253 0.74 +0.7
2,364,815 - arnB b2253 0.76 -0.3
2,364,844 + arnB b2253 0.78 +0.6
2,364,844 + arnB b2253 0.78 +0.0
2,364,857 - arnB b2253 0.80 +0.5
2,364,863 + arnB b2253 0.80 +1.2
2,364,863 + arnB b2253 0.80 -0.2
2,364,918 - arnB b2253 0.85 -1.3
2,364,926 + arnB b2253 0.86 +0.8
2,364,926 + arnB b2253 0.86 +1.3
2,365,002 + +0.6
2,365,021 + -0.9
2,365,035 - +0.2
2,365,053 + -0.8
2,365,053 + -2.2
2,365,056 + +0.1
2,365,179 - +1.1
2,365,181 + -1.1
2,365,198 - yfbF b2254 0.11 -1.5
2,365,200 + yfbF b2254 0.11 -0.3
2,365,208 - yfbF b2254 0.12 +1.0
2,365,220 - yfbF b2254 0.13 +2.5
2,365,264 - yfbF b2254 0.18 +0.2
2,365,290 - yfbF b2254 0.20 -1.1
2,365,290 - yfbF b2254 0.20 -1.1
2,365,414 + yfbF b2254 0.33 -2.3
2,365,433 + yfbF b2254 0.35 -1.0
2,365,450 + yfbF b2254 0.37 +0.6
2,365,477 - yfbF b2254 0.40 +0.3
2,365,529 + yfbF b2254 0.45 +1.9
2,365,555 + yfbF b2254 0.48 -0.7
2,365,555 + yfbF b2254 0.48 -0.6
2,365,637 + yfbF b2254 0.56 -1.1
2,365,773 + yfbF b2254 0.70 +0.1
2,365,786 - yfbF b2254 0.72 +1.1
2,365,786 - yfbF b2254 0.72 +0.3
2,365,804 - yfbF b2254 0.73 +0.6
2,365,854 - yfbF b2254 0.79 +0.2
2,365,866 + yfbF b2254 0.80 -1.3
2,365,866 + yfbF b2254 0.80 -0.9
2,365,965 - +1.4
2,365,983 + -0.2
2,365,984 + -0.6
2,365,984 + +1.7
2,366,037 - +2.2

Or see this region's nucleotide sequence