Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dld and pbpG are separated by 37 nucleotides pbpG and yohC are separated by 173 nucleotides yohC and yohD are separated by 169 nucleotides
b2133: dld - D-lactate dehydrogenase, FAD-binding, NADH independent (NCBI), at 2,220,207 to 2,221,922
dld
b2134: pbpG - penicillin-binding protein 7 (VIMSS), at 2,221,960 to 2,222,892
pbpG
b2135: yohC - orf, hypothetical protein (VIMSS), at 2,223,066 to 2,223,653
yohC
b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401
yohD
Position (kb)
2221
2222
2223 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2221.087 kb on + strand, within dld at 2221.199 kb on + strand, within dld at 2221.199 kb on + strand, within dld at 2221.333 kb on + strand, within dld at 2221.333 kb on + strand, within dld at 2221.373 kb on + strand, within dld at 2221.373 kb on + strand, within dld at 2221.482 kb on - strand, within dld at 2221.505 kb on - strand, within dld at 2221.577 kb on + strand, within dld at 2221.658 kb on - strand, within dld at 2221.687 kb on + strand, within dld at 2221.697 kb on + strand, within dld at 2221.697 kb on + strand, within dld at 2221.730 kb on - strand, within dld at 2221.780 kb on + strand at 2221.781 kb on - strand at 2221.938 kb on - strand at 2222.020 kb on + strand at 2222.020 kb on + strand at 2222.154 kb on + strand, within pbpG at 2222.163 kb on + strand, within pbpG at 2222.171 kb on - strand, within pbpG at 2222.183 kb on + strand, within pbpG at 2222.254 kb on - strand, within pbpG at 2222.306 kb on + strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.314 kb on - strand, within pbpG at 2222.552 kb on - strand, within pbpG at 2222.552 kb on - strand, within pbpG at 2222.602 kb on - strand, within pbpG at 2222.804 kb on - strand at 2222.806 kb on - strand at 2222.976 kb on - strand at 2223.193 kb on - strand, within yohC at 2223.323 kb on - strand, within yohC at 2223.457 kb on - strand, within yohC at 2223.476 kb on - strand, within yohC at 2223.531 kb on + strand, within yohC at 2223.573 kb on - strand, within yohC at 2223.647 kb on + strand at 2223.831 kb on + strand at 2223.879 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 2,221,087 + dld b2133 0.51 -1.5 2,221,199 + dld b2133 0.58 -0.1 2,221,199 + dld b2133 0.58 -0.8 2,221,333 + dld b2133 0.66 -1.5 2,221,333 + dld b2133 0.66 +1.2 2,221,373 + dld b2133 0.68 -2.2 2,221,373 + dld b2133 0.68 -0.2 2,221,482 - dld b2133 0.74 +0.4 2,221,505 - dld b2133 0.76 -2.0 2,221,577 + dld b2133 0.80 -1.7 2,221,658 - dld b2133 0.85 -1.4 2,221,687 + dld b2133 0.86 +0.0 2,221,697 + dld b2133 0.87 +0.5 2,221,697 + dld b2133 0.87 +0.4 2,221,730 - dld b2133 0.89 -1.0 2,221,780 + -2.3 2,221,781 - -0.0 2,221,938 - -1.7 2,222,020 + -0.9 2,222,020 + -1.8 2,222,154 + pbpG b2134 0.21 -0.3 2,222,163 + pbpG b2134 0.22 -0.3 2,222,171 - pbpG b2134 0.23 +2.1 2,222,183 + pbpG b2134 0.24 +1.7 2,222,254 - pbpG b2134 0.32 -1.3 2,222,306 + pbpG b2134 0.37 +1.0 2,222,314 - pbpG b2134 0.38 -2.7 2,222,314 - pbpG b2134 0.38 +0.4 2,222,314 - pbpG b2134 0.38 +0.8 2,222,314 - pbpG b2134 0.38 -0.8 2,222,552 - pbpG b2134 0.63 -0.2 2,222,552 - pbpG b2134 0.63 -0.7 2,222,602 - pbpG b2134 0.69 -0.8 2,222,804 - -1.5 2,222,806 - +1.5 2,222,976 - -2.0 2,223,193 - yohC b2135 0.22 +0.7 2,223,323 - yohC b2135 0.44 -1.4 2,223,457 - yohC b2135 0.66 +1.0 2,223,476 - yohC b2135 0.70 +0.4 2,223,531 + yohC b2135 0.79 +1.6 2,223,573 - yohC b2135 0.86 +1.7 2,223,647 + -1.7 2,223,831 + -1.4 2,223,879 + -1.1
Or see this region's nucleotide sequence