Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bisZ and torY are separated by 24 nucleotides torY and cutC are separated by 387 nucleotides
b1872: bisZ - biotin sulfoxide reductase 2 (VIMSS), at 1,952,602 to 1,955,031
bisZ
b1873: torY - TMAO reductase III (TorYZ), cytochrome c-type subunit (NCBI), at 1,955,056 to 1,956,156
torY
b1874: cutC - copper homeostasis protein (NCBI), at 1,956,544 to 1,957,290
cutC
Position (kb)
1955
1956
1957 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1954.203 kb on + strand, within bisZ at 1954.349 kb on + strand, within bisZ at 1954.357 kb on - strand, within bisZ at 1954.357 kb on - strand, within bisZ at 1954.375 kb on + strand, within bisZ at 1954.401 kb on + strand, within bisZ at 1954.592 kb on - strand, within bisZ at 1954.698 kb on + strand, within bisZ at 1954.803 kb on - strand at 1954.951 kb on + strand at 1954.951 kb on + strand at 1954.984 kb on + strand at 1954.984 kb on + strand at 1955.019 kb on + strand at 1955.069 kb on - strand at 1955.180 kb on + strand, within torY at 1955.216 kb on + strand, within torY at 1955.216 kb on + strand, within torY at 1955.461 kb on - strand, within torY at 1955.485 kb on + strand, within torY at 1955.485 kb on + strand, within torY at 1955.507 kb on + strand, within torY at 1955.507 kb on + strand, within torY at 1955.530 kb on - strand, within torY at 1955.580 kb on - strand, within torY at 1955.599 kb on + strand, within torY at 1955.607 kb on + strand, within torY at 1955.669 kb on - strand, within torY at 1955.669 kb on - strand, within torY at 1955.672 kb on + strand, within torY at 1955.686 kb on - strand, within torY at 1955.733 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.774 kb on + strand, within torY at 1955.800 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.844 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.969 kb on - strand, within torY at 1955.974 kb on - strand, within torY at 1956.405 kb on - strand at 1956.407 kb on - strand at 1956.407 kb on - strand at 1956.416 kb on - strand at 1956.416 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.417 kb on - strand at 1956.505 kb on - strand at 1956.561 kb on + strand at 1956.628 kb on + strand, within cutC at 1956.628 kb on + strand, within cutC at 1956.709 kb on + strand, within cutC at 1956.764 kb on - strand, within cutC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 1,954,203 + bisZ b1872 0.66 +0.1 1,954,349 + bisZ b1872 0.72 -0.4 1,954,357 - bisZ b1872 0.72 -0.0 1,954,357 - bisZ b1872 0.72 +0.7 1,954,375 + bisZ b1872 0.73 +2.2 1,954,401 + bisZ b1872 0.74 -2.3 1,954,592 - bisZ b1872 0.82 +2.2 1,954,698 + bisZ b1872 0.86 +0.1 1,954,803 - +0.5 1,954,951 + +0.9 1,954,951 + +1.4 1,954,984 + +0.7 1,954,984 + +2.3 1,955,019 + -0.9 1,955,069 - +0.1 1,955,180 + torY b1873 0.11 -1.6 1,955,216 + torY b1873 0.15 +3.0 1,955,216 + torY b1873 0.15 +0.5 1,955,461 - torY b1873 0.37 -1.4 1,955,485 + torY b1873 0.39 -0.9 1,955,485 + torY b1873 0.39 +0.3 1,955,507 + torY b1873 0.41 -0.1 1,955,507 + torY b1873 0.41 +0.1 1,955,530 - torY b1873 0.43 +0.9 1,955,580 - torY b1873 0.48 +0.7 1,955,599 + torY b1873 0.49 +0.0 1,955,607 + torY b1873 0.50 -0.5 1,955,669 - torY b1873 0.56 -0.9 1,955,669 - torY b1873 0.56 -1.3 1,955,672 + torY b1873 0.56 -0.3 1,955,686 - torY b1873 0.57 -0.3 1,955,733 + torY b1873 0.61 -0.9 1,955,765 + torY b1873 0.64 +2.0 1,955,765 + torY b1873 0.64 +2.2 1,955,774 + torY b1873 0.65 -0.3 1,955,800 + torY b1873 0.68 -0.9 1,955,820 + torY b1873 0.69 -1.9 1,955,820 + torY b1873 0.69 -0.0 1,955,844 + torY b1873 0.72 -0.9 1,955,854 + torY b1873 0.72 +0.7 1,955,854 + torY b1873 0.72 +0.7 1,955,969 - torY b1873 0.83 +0.7 1,955,974 - torY b1873 0.83 +0.7 1,956,405 - -0.1 1,956,407 - +3.5 1,956,407 - -0.9 1,956,416 - -0.3 1,956,416 - +0.7 1,956,417 - +0.4 1,956,417 - +1.1 1,956,417 - -0.3 1,956,417 - +0.9 1,956,417 - -0.9 1,956,417 - -1.3 1,956,417 - +0.1 1,956,505 - -4.1 1,956,561 + +0.7 1,956,628 + cutC b1874 0.11 -1.9 1,956,628 + cutC b1874 0.11 +1.3 1,956,709 + cutC b1874 0.22 -2.3 1,956,764 - cutC b1874 0.29 +0.2
Or see this region's nucleotide sequence