Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yecP and bisZ are separated by 164 nucleotides bisZ and torY are separated by 24 nucleotides
b1871: yecP - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 1,951,466 to 1,952,437
yecP
b1872: bisZ - biotin sulfoxide reductase 2 (VIMSS), at 1,952,602 to 1,955,031
bisZ
b1873: torY - TMAO reductase III (TorYZ), cytochrome c-type subunit (NCBI), at 1,955,056 to 1,956,156
torY
Position (kb)
1952
1953
1954
1955
1956 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1951.831 kb on - strand, within yecP at 1951.831 kb on - strand, within yecP at 1951.834 kb on + strand, within yecP at 1951.834 kb on + strand, within yecP at 1951.868 kb on + strand, within yecP at 1951.912 kb on + strand, within yecP at 1951.912 kb on + strand, within yecP at 1951.920 kb on + strand, within yecP at 1952.017 kb on - strand, within yecP at 1952.017 kb on - strand, within yecP at 1952.288 kb on - strand, within yecP at 1952.330 kb on - strand, within yecP at 1952.330 kb on - strand, within yecP at 1952.419 kb on + strand at 1952.530 kb on + strand at 1952.786 kb on + strand at 1952.794 kb on - strand at 1952.794 kb on - strand at 1952.972 kb on + strand, within bisZ at 1952.986 kb on + strand, within bisZ at 1953.062 kb on - strand, within bisZ at 1953.078 kb on + strand, within bisZ at 1953.208 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.262 kb on - strand, within bisZ at 1953.270 kb on + strand, within bisZ at 1953.298 kb on - strand, within bisZ at 1953.467 kb on - strand, within bisZ at 1953.554 kb on + strand, within bisZ at 1953.554 kb on + strand, within bisZ at 1953.674 kb on + strand, within bisZ at 1953.692 kb on + strand, within bisZ at 1953.899 kb on - strand, within bisZ at 1953.899 kb on - strand, within bisZ at 1953.903 kb on + strand, within bisZ at 1954.203 kb on + strand, within bisZ at 1954.349 kb on + strand, within bisZ at 1954.357 kb on - strand, within bisZ at 1954.357 kb on - strand, within bisZ at 1954.375 kb on + strand, within bisZ at 1954.401 kb on + strand, within bisZ at 1954.592 kb on - strand, within bisZ at 1954.698 kb on + strand, within bisZ at 1954.803 kb on - strand at 1954.951 kb on + strand at 1954.951 kb on + strand at 1954.984 kb on + strand at 1954.984 kb on + strand at 1955.019 kb on + strand at 1955.069 kb on - strand at 1955.180 kb on + strand, within torY at 1955.216 kb on + strand, within torY at 1955.216 kb on + strand, within torY at 1955.461 kb on - strand, within torY at 1955.485 kb on + strand, within torY at 1955.485 kb on + strand, within torY at 1955.507 kb on + strand, within torY at 1955.507 kb on + strand, within torY at 1955.530 kb on - strand, within torY at 1955.580 kb on - strand, within torY at 1955.599 kb on + strand, within torY at 1955.607 kb on + strand, within torY at 1955.669 kb on - strand, within torY at 1955.669 kb on - strand, within torY at 1955.672 kb on + strand, within torY at 1955.686 kb on - strand, within torY at 1955.733 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.765 kb on + strand, within torY at 1955.774 kb on + strand, within torY at 1955.800 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.820 kb on + strand, within torY at 1955.844 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.854 kb on + strand, within torY at 1955.969 kb on - strand, within torY at 1955.974 kb on - strand, within torY
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 1,951,831 - yecP b1871 0.38 +0.5 1,951,831 - yecP b1871 0.38 +1.9 1,951,834 + yecP b1871 0.38 -2.1 1,951,834 + yecP b1871 0.38 -1.6 1,951,868 + yecP b1871 0.41 -1.9 1,951,912 + yecP b1871 0.46 -0.3 1,951,912 + yecP b1871 0.46 -1.6 1,951,920 + yecP b1871 0.47 -2.6 1,952,017 - yecP b1871 0.57 -0.6 1,952,017 - yecP b1871 0.57 +2.3 1,952,288 - yecP b1871 0.85 -1.1 1,952,330 - yecP b1871 0.89 -1.9 1,952,330 - yecP b1871 0.89 +1.5 1,952,419 + -0.2 1,952,530 + -1.3 1,952,786 + -1.3 1,952,794 - -0.5 1,952,794 - -0.9 1,952,972 + bisZ b1872 0.15 +2.1 1,952,986 + bisZ b1872 0.16 +0.6 1,953,062 - bisZ b1872 0.19 -0.1 1,953,078 + bisZ b1872 0.20 +0.1 1,953,208 - bisZ b1872 0.25 +0.7 1,953,262 - bisZ b1872 0.27 -0.2 1,953,262 - bisZ b1872 0.27 -0.9 1,953,262 - bisZ b1872 0.27 -1.5 1,953,270 + bisZ b1872 0.27 -1.6 1,953,298 - bisZ b1872 0.29 -0.9 1,953,467 - bisZ b1872 0.36 +1.3 1,953,554 + bisZ b1872 0.39 +0.2 1,953,554 + bisZ b1872 0.39 -0.9 1,953,674 + bisZ b1872 0.44 +0.3 1,953,692 + bisZ b1872 0.45 +0.4 1,953,899 - bisZ b1872 0.53 -0.9 1,953,899 - bisZ b1872 0.53 -0.3 1,953,903 + bisZ b1872 0.54 -0.7 1,954,203 + bisZ b1872 0.66 +0.1 1,954,349 + bisZ b1872 0.72 -0.4 1,954,357 - bisZ b1872 0.72 -0.0 1,954,357 - bisZ b1872 0.72 +0.7 1,954,375 + bisZ b1872 0.73 +2.2 1,954,401 + bisZ b1872 0.74 -2.3 1,954,592 - bisZ b1872 0.82 +2.2 1,954,698 + bisZ b1872 0.86 +0.1 1,954,803 - +0.5 1,954,951 + +0.9 1,954,951 + +1.4 1,954,984 + +0.7 1,954,984 + +2.3 1,955,019 + -0.9 1,955,069 - +0.1 1,955,180 + torY b1873 0.11 -1.6 1,955,216 + torY b1873 0.15 +3.0 1,955,216 + torY b1873 0.15 +0.5 1,955,461 - torY b1873 0.37 -1.4 1,955,485 + torY b1873 0.39 -0.9 1,955,485 + torY b1873 0.39 +0.3 1,955,507 + torY b1873 0.41 -0.1 1,955,507 + torY b1873 0.41 +0.1 1,955,530 - torY b1873 0.43 +0.9 1,955,580 - torY b1873 0.48 +0.7 1,955,599 + torY b1873 0.49 +0.0 1,955,607 + torY b1873 0.50 -0.5 1,955,669 - torY b1873 0.56 -0.9 1,955,669 - torY b1873 0.56 -1.3 1,955,672 + torY b1873 0.56 -0.3 1,955,686 - torY b1873 0.57 -0.3 1,955,733 + torY b1873 0.61 -0.9 1,955,765 + torY b1873 0.64 +2.0 1,955,765 + torY b1873 0.64 +2.2 1,955,774 + torY b1873 0.65 -0.3 1,955,800 + torY b1873 0.68 -0.9 1,955,820 + torY b1873 0.69 -1.9 1,955,820 + torY b1873 0.69 -0.0 1,955,844 + torY b1873 0.72 -0.9 1,955,854 + torY b1873 0.72 +0.7 1,955,854 + torY b1873 0.72 +0.7 1,955,969 - torY b1873 0.83 +0.7 1,955,974 - torY b1873 0.83 +0.7
Or see this region's nucleotide sequence