Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaQ and yoaG are separated by 147 nucleotides yoaG and yeaR are separated by 3 nucleotides yeaR and yeaS are separated by 172 nucleotides yeaS and yeaT are separated by 126 nucleotides
b1795: yeaQ - conserved inner membrane protein (NCBI), at 1,877,031 to 1,877,279
yeaQ
b1796: yoaG - hypothetical protein (NCBI), at 1,877,427 to 1,877,609
yoaG
b1797: yeaR - hypothetical protein (NCBI), at 1,877,613 to 1,877,972
yeaR
b1798: yeaS - neutral amino-acid efflux system (NCBI), at 1,878,145 to 1,878,783
yeaS
b1799: yeaT - putative transcriptional regulator LYSR-type (VIMSS), at 1,878,910 to 1,879,833
yeaT
Position (kb)
1878
1879 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1877.145 kb on - strand, within yeaQ at 1877.145 kb on - strand, within yeaQ at 1877.174 kb on - strand, within yeaQ at 1877.175 kb on - strand, within yeaQ at 1877.260 kb on + strand at 1877.433 kb on + strand at 1877.476 kb on + strand, within yoaG at 1877.476 kb on + strand, within yoaG at 1877.484 kb on - strand, within yoaG at 1877.513 kb on - strand, within yoaG at 1877.571 kb on - strand, within yoaG at 1877.602 kb on - strand at 1877.658 kb on - strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.714 kb on + strand, within yeaR at 1877.715 kb on - strand, within yeaR at 1877.764 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.791 kb on + strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.814 kb on - strand, within yeaR at 1877.859 kb on - strand, within yeaR at 1877.985 kb on + strand at 1878.018 kb on - strand at 1878.085 kb on - strand at 1878.142 kb on - strand at 1878.142 kb on - strand at 1878.186 kb on + strand at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.303 kb on - strand, within yeaS at 1878.340 kb on + strand, within yeaS at 1878.363 kb on + strand, within yeaS at 1878.363 kb on + strand, within yeaS at 1878.364 kb on - strand, within yeaS at 1878.388 kb on - strand, within yeaS at 1878.414 kb on - strand, within yeaS at 1878.446 kb on + strand, within yeaS at 1878.454 kb on - strand, within yeaS at 1878.484 kb on + strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.511 kb on + strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.608 kb on - strand, within yeaS at 1878.656 kb on - strand, within yeaS at 1878.664 kb on - strand, within yeaS at 1878.721 kb on - strand at 1878.738 kb on + strand at 1878.746 kb on - strand at 1878.752 kb on - strand at 1878.757 kb on + strand at 1878.757 kb on + strand at 1878.841 kb on + strand at 1878.940 kb on + strand at 1879.004 kb on - strand, within yeaT at 1879.009 kb on + strand, within yeaT at 1879.027 kb on - strand, within yeaT at 1879.118 kb on - strand, within yeaT at 1879.175 kb on - strand, within yeaT at 1879.175 kb on - strand, within yeaT at 1879.210 kb on - strand, within yeaT at 1879.210 kb on - strand, within yeaT at 1879.231 kb on - strand, within yeaT at 1879.245 kb on + strand, within yeaT at 1879.253 kb on - strand, within yeaT at 1879.266 kb on + strand, within yeaT at 1879.340 kb on - strand, within yeaT at 1879.340 kb on - strand, within yeaT at 1879.410 kb on + strand, within yeaT at 1879.426 kb on + strand, within yeaT at 1879.601 kb on - strand, within yeaT
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 1,877,145 - yeaQ b1795 0.46 -0.3 1,877,145 - yeaQ b1795 0.46 +0.4 1,877,174 - yeaQ b1795 0.57 -0.2 1,877,175 - yeaQ b1795 0.58 +0.3 1,877,260 + +0.4 1,877,433 + -2.9 1,877,476 + yoaG b1796 0.27 -1.6 1,877,476 + yoaG b1796 0.27 +1.1 1,877,484 - yoaG b1796 0.31 +0.8 1,877,513 - yoaG b1796 0.47 +0.2 1,877,571 - yoaG b1796 0.79 -1.0 1,877,602 - -0.2 1,877,658 - yeaR b1797 0.12 -3.2 1,877,663 + yeaR b1797 0.14 +0.2 1,877,663 + yeaR b1797 0.14 +1.2 1,877,714 + yeaR b1797 0.28 -1.0 1,877,715 - yeaR b1797 0.28 -0.9 1,877,764 + yeaR b1797 0.42 -0.1 1,877,765 + yeaR b1797 0.42 +0.5 1,877,765 + yeaR b1797 0.42 -2.4 1,877,791 + yeaR b1797 0.49 -0.5 1,877,804 - yeaR b1797 0.53 +0.1 1,877,804 - yeaR b1797 0.53 -0.9 1,877,804 - yeaR b1797 0.53 -1.3 1,877,814 - yeaR b1797 0.56 -0.3 1,877,859 - yeaR b1797 0.68 +1.6 1,877,985 + +0.1 1,878,018 - -0.6 1,878,085 - +0.1 1,878,142 - -1.0 1,878,142 - -0.7 1,878,186 + +0.7 1,878,236 + yeaS b1798 0.14 +0.7 1,878,236 + yeaS b1798 0.14 +0.7 1,878,236 + yeaS b1798 0.14 +0.7 1,878,236 + yeaS b1798 0.14 -0.3 1,878,244 - yeaS b1798 0.15 +1.3 1,878,244 - yeaS b1798 0.15 -1.1 1,878,244 - yeaS b1798 0.15 +1.7 1,878,244 - yeaS b1798 0.15 -0.3 1,878,303 - yeaS b1798 0.25 +3.3 1,878,340 + yeaS b1798 0.31 -0.3 1,878,363 + yeaS b1798 0.34 +0.7 1,878,363 + yeaS b1798 0.34 -0.9 1,878,364 - yeaS b1798 0.34 +1.4 1,878,388 - yeaS b1798 0.38 -0.3 1,878,414 - yeaS b1798 0.42 -1.1 1,878,446 + yeaS b1798 0.47 -0.3 1,878,454 - yeaS b1798 0.48 +0.7 1,878,484 + yeaS b1798 0.53 +0.7 1,878,492 - yeaS b1798 0.54 +0.3 1,878,492 - yeaS b1798 0.54 +0.1 1,878,492 - yeaS b1798 0.54 -1.3 1,878,511 + yeaS b1798 0.57 +0.7 1,878,598 - yeaS b1798 0.71 +0.9 1,878,598 - yeaS b1798 0.71 +1.5 1,878,598 - yeaS b1798 0.71 +0.7 1,878,598 - yeaS b1798 0.71 +1.9 1,878,608 - yeaS b1798 0.72 +0.3 1,878,656 - yeaS b1798 0.80 +2.5 1,878,664 - yeaS b1798 0.81 +1.2 1,878,721 - -0.3 1,878,738 + +0.7 1,878,746 - -0.3 1,878,752 - -0.9 1,878,757 + +0.7 1,878,757 + +0.7 1,878,841 + +0.7 1,878,940 + +0.7 1,879,004 - yeaT b1799 0.10 -0.3 1,879,009 + yeaT b1799 0.11 +0.7 1,879,027 - yeaT b1799 0.13 -1.3 1,879,118 - yeaT b1799 0.23 -0.9 1,879,175 - yeaT b1799 0.29 -1.6 1,879,175 - yeaT b1799 0.29 +2.0 1,879,210 - yeaT b1799 0.32 +0.7 1,879,210 - yeaT b1799 0.32 +3.9 1,879,231 - yeaT b1799 0.35 -0.0 1,879,245 + yeaT b1799 0.36 -0.3 1,879,253 - yeaT b1799 0.37 +0.7 1,879,266 + yeaT b1799 0.39 -0.9 1,879,340 - yeaT b1799 0.47 +3.3 1,879,340 - yeaT b1799 0.47 +1.7 1,879,410 + yeaT b1799 0.54 +0.7 1,879,426 + yeaT b1799 0.56 -0.3 1,879,601 - yeaT b1799 0.75 -1.1
Or see this region's nucleotide sequence