Strain Fitness in Escherichia coli BW25113 around b1188

Experiment: LB Aerobic with Irgasan 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntnhaB and fadR are separated by 220 nucleotidesfadR and ycgB are separated by 51 nucleotidesycgB and dadA are separated by 329 nucleotides b1186: nhaB - NhaB sodium/proton transporter (Katherine Huang), at 1,232,399 to 1,233,940 nhaB b1187: fadR - fatty acid metabolism regulator (NCBI), at 1,234,161 to 1,234,880 fadR b1188: ycgB - ycgB component of nitrogen-related signalling system (of yeaGH-ycgB) (from data), at 1,234,932 to 1,236,464 ycgB b1189: dadA - D-amino acid dehydrogenase (quinone) DadA (EC 1.4.5.1) (from data), at 1,236,794 to 1,238,092 dadA Position (kb) 1234 1235 1236 1237Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5 6 7 8 9at 1234.004 kb on - strandat 1234.099 kb on - strandat 1234.102 kb on + strandat 1234.112 kb on + strandat 1234.114 kb on + strandat 1234.118 kb on - strandat 1234.118 kb on - strandat 1234.157 kb on + strandat 1234.222 kb on + strandat 1234.299 kb on + strand, within fadRat 1234.317 kb on - strand, within fadRat 1234.331 kb on + strand, within fadRat 1234.436 kb on - strandat 1234.565 kb on + strand, within fadRat 1234.673 kb on + strand, within fadRat 1234.681 kb on - strand, within fadRat 1234.720 kb on + strand, within fadRat 1234.807 kb on + strand, within fadRat 1234.807 kb on + strand, within fadRat 1234.815 kb on - strandat 1234.889 kb on - strandat 1234.989 kb on + strandat 1235.038 kb on - strandat 1235.069 kb on + strandat 1235.092 kb on - strand, within ycgBat 1235.092 kb on - strand, within ycgBat 1235.136 kb on - strand, within ycgBat 1235.251 kb on + strand, within ycgBat 1235.263 kb on + strand, within ycgBat 1235.263 kb on + strand, within ycgBat 1235.362 kb on - strand, within ycgBat 1235.483 kb on - strand, within ycgBat 1235.815 kb on - strand, within ycgBat 1235.815 kb on - strand, within ycgBat 1236.122 kb on + strand, within ycgBat 1236.187 kb on - strand, within ycgBat 1236.339 kb on - strandat 1236.345 kb on - strandat 1236.374 kb on + strandat 1236.374 kb on + strandat 1236.414 kb on - strandat 1236.456 kb on - strandat 1236.788 kb on - strandat 1236.788 kb on - strandat 1236.803 kb on + strandat 1236.924 kb on - strand, within dadAat 1237.037 kb on + strand, within dadAat 1237.040 kb on - strand, within dadAat 1237.146 kb on + strand, within dadAat 1237.180 kb on + strand, within dadAat 1237.213 kb on + strand, within dadAat 1237.213 kb on + strand, within dadAat 1237.218 kb on + strand, within dadA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM
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1,234,004 - +0.6
1,234,099 - +1.4
1,234,102 + +0.9
1,234,112 + -1.7
1,234,114 + -1.3
1,234,118 - -1.5
1,234,118 - +0.1
1,234,157 + -2.3
1,234,222 + +8.3
1,234,299 + fadR b1187 0.19 +8.9
1,234,317 - fadR b1187 0.22 +7.0
1,234,331 + fadR b1187 0.24 +7.7
1,234,436 - +0.7
1,234,565 + fadR b1187 0.56 +8.1
1,234,673 + fadR b1187 0.71 +7.8
1,234,681 - fadR b1187 0.72 +7.5
1,234,720 + fadR b1187 0.78 +7.5
1,234,807 + fadR b1187 0.90 +8.5
1,234,807 + fadR b1187 0.90 +7.8
1,234,815 - +7.0
1,234,889 - +5.6
1,234,989 + +0.5
1,235,038 - -0.6
1,235,069 + +1.9
1,235,092 - ycgB b1188 0.10 -0.2
1,235,092 - ycgB b1188 0.10 -0.2
1,235,136 - ycgB b1188 0.13 -0.3
1,235,251 + ycgB b1188 0.21 -0.9
1,235,263 + ycgB b1188 0.22 -1.3
1,235,263 + ycgB b1188 0.22 +0.4
1,235,362 - ycgB b1188 0.28 -1.6
1,235,483 - ycgB b1188 0.36 +1.7
1,235,815 - ycgB b1188 0.58 -0.3
1,235,815 - ycgB b1188 0.58 +0.8
1,236,122 + ycgB b1188 0.78 -0.1
1,236,187 - ycgB b1188 0.82 +0.7
1,236,339 - +0.4
1,236,345 - +2.0
1,236,374 + +1.8
1,236,374 + +1.4
1,236,414 - -2.1
1,236,456 - -0.2
1,236,788 - -0.9
1,236,788 - +1.5
1,236,803 + -0.1
1,236,924 - dadA b1189 0.10 +1.9
1,237,037 + dadA b1189 0.19 -1.0
1,237,040 - dadA b1189 0.19 -0.2
1,237,146 + dadA b1189 0.27 +0.6
1,237,180 + dadA b1189 0.30 -0.4
1,237,213 + dadA b1189 0.32 -1.9
1,237,213 + dadA b1189 0.32 +0.7
1,237,218 + dadA b1189 0.33 +0.6

Or see this region's nucleotide sequence