Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabH and fabD are separated by 15 nucleotides fabD and fabG are separated by 12 nucleotides fabG and acpP are separated by 210 nucleotides acpP and fabF are separated by 87 nucleotides
b1091: fabH - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,147,982 to 1,148,935
fabH
b1092: fabD - acyl carrier protein S-malonyltransferase (NCBI), at 1,148,951 to 1,149,880
fabD
b1093: fabG - 3-oxoacyl-[acyl-carrier-protein] reductase (NCBI), at 1,149,893 to 1,150,627
fabG
b1094: acpP - acyl carrier protein (NCBI), at 1,150,838 to 1,151,074
acpP
b1095: fabF - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,151,162 to 1,152,403
fabF
Position (kb)
1149
1150
1151 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 1149.875 kb on + strand at 1151.238 kb on + strand at 1151.266 kb on + strand at 1151.266 kb on + strand at 1151.368 kb on - strand, within fabF at 1151.416 kb on - strand, within fabF at 1151.427 kb on - strand, within fabF at 1151.427 kb on - strand, within fabF at 1151.530 kb on - strand, within fabF at 1151.530 kb on - strand, within fabF at 1151.535 kb on - strand, within fabF at 1151.535 kb on - strand, within fabF at 1151.608 kb on + strand, within fabF at 1151.608 kb on + strand, within fabF at 1151.610 kb on + strand, within fabF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 1,149,875 + -0.3 1,151,238 + +4.1 1,151,266 + +3.7 1,151,266 + +4.2 1,151,368 - fabF b1095 0.17 +2.7 1,151,416 - fabF b1095 0.20 +2.1 1,151,427 - fabF b1095 0.21 +5.5 1,151,427 - fabF b1095 0.21 +4.9 1,151,530 - fabF b1095 0.30 +5.0 1,151,530 - fabF b1095 0.30 +4.2 1,151,535 - fabF b1095 0.30 +5.9 1,151,535 - fabF b1095 0.30 +3.7 1,151,608 + fabF b1095 0.36 +3.8 1,151,608 + fabF b1095 0.36 +4.6 1,151,610 + fabF b1095 0.36 +2.9
Or see this region's nucleotide sequence