Strain Fitness in Escherichia coli BW25113 around b0960

Experiment: LB Aerobic with Irgasan 0.0000003125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Irgasan 0.0000003125 mM
1,020,001	-	sulA	b0958	0.72	-0.5
1,020,001	-	sulA	b0958	0.72	+2.2
1,020,042	+	sulA	b0958	0.80	-2.2
1,020,049	+	sulA	b0958	0.82	+2.8
1,020,049	+	sulA	b0958	0.82	+1.1
1,020,226	-				+0.7
1,020,419	+				+0.9
1,020,453	+	yccR	b0959	0.15	+0.4
1,020,496	-	yccR	b0959	0.21	-2.2
1,020,496	-	yccR	b0959	0.21	-0.2
1,020,529	+	yccR	b0959	0.27	-0.0
1,020,529	+	yccR	b0959	0.27	+0.1
1,020,534	+	yccR	b0959	0.27	-1.4
1,020,534	+	yccR	b0959	0.27	-2.0
1,020,558	-	yccR	b0959	0.31	+1.0
1,020,597	-	yccR	b0959	0.37	+0.9
1,020,605	-	yccR	b0959	0.39	+0.6
1,020,640	+	yccR	b0959	0.44	-0.7
1,020,799	-	yccR	b0959	0.70	+0.4
1,020,800	-	yccR	b0959	0.70	-0.0
1,020,894	+	yccR	b0959	0.85	-1.4
1,020,983	-				-2.8
1,020,998	-				+1.0
1,021,034	-				+0.5
1,021,151	-				-2.5
1,021,223	-	b0960	b0960	0.13	-4.6
1,021,267	+	b0960	b0960	0.15	+0.8
1,021,267	+	b0960	b0960	0.15	-1.1
1,021,335	+	b0960	b0960	0.18	-0.8
1,021,342	-	b0960	b0960	0.18	-4.3
1,021,377	-	b0960	b0960	0.20	-3.2
1,021,441	-	b0960	b0960	0.23	-2.5
1,021,473	-	b0960	b0960	0.24	-1.8
1,021,483	+	b0960	b0960	0.25	-3.3
1,021,483	+	b0960	b0960	0.25	-3.7
1,021,526	-	b0960	b0960	0.27	-2.9
1,021,526	-	b0960	b0960	0.27	-2.4
1,021,558	-	b0960	b0960	0.28	-1.2
1,021,624	-	b0960	b0960	0.31	-1.9
1,021,661	-	b0960	b0960	0.33	-1.2
1,021,751	+	b0960	b0960	0.37	-1.8
1,021,751	+	b0960	b0960	0.37	-1.8
1,021,764	-	b0960	b0960	0.38	-2.9
1,021,920	+	b0960	b0960	0.45	-1.5
1,021,920	+	b0960	b0960	0.45	-1.2
1,021,983	-	b0960	b0960	0.48	-3.2
1,021,983	-	b0960	b0960	0.48	-1.2
1,022,028	+	b0960	b0960	0.50	-1.0
1,022,033	+	b0960	b0960	0.50	-2.4
1,022,053	+	b0960	b0960	0.51	-4.3
1,022,308	+	b0960	b0960	0.63	-2.5
1,022,316	-	b0960	b0960	0.63	-2.7
1,022,316	-	b0960	b0960	0.63	-0.7
1,022,336	-	b0960	b0960	0.64	-1.5
1,022,444	-	b0960	b0960	0.69	-0.5
1,022,444	-	b0960	b0960	0.69	-2.0
1,022,446	-	b0960	b0960	0.69	-1.7
1,022,512	+	b0960	b0960	0.72	-1.0
1,022,512	-	b0960	b0960	0.72	-3.0
1,022,565	+	b0960	b0960	0.75	-2.4
1,022,602	-	b0960	b0960	0.77	-0.2
1,022,603	+	b0960	b0960	0.77	-1.8
1,022,603	+	b0960	b0960	0.77	-1.5
1,022,644	+	b0960	b0960	0.79	-2.5
1,022,703	+	b0960	b0960	0.81	-1.2
1,022,713	-	b0960	b0960	0.82	-2.3
1,022,810	+	b0960	b0960	0.86	+2.4
1,022,819	+	b0960	b0960	0.87	-2.4
1,022,852	-	b0960	b0960	0.88	-1.8
1,022,884	-	b0960	b0960	0.90	-1.4
1,022,884	-	b0960	b0960	0.90	-3.4
1,022,886	+	b0960	b0960	0.90	-2.3
1,023,008	+				-1.8
1,023,160	+				-0.8
1,023,160	+				-1.5
1,023,170	-	yccF	b0961	0.10	+0.3
1,023,179	-	yccF	b0961	0.12	+1.9
1,023,400	+	yccF	b0961	0.62	-3.3
1,023,423	+	yccF	b0961	0.67	-2.0
1,023,423	+	yccF	b0961	0.67	-1.8
1,023,488	+	yccF	b0961	0.81	-2.8
1,023,512	+	yccF	b0961	0.87	-0.4
1,023,525	-	yccF	b0961	0.89	-2.0
1,023,525	-	yccF	b0961	0.89	-1.1
1,023,588	-				+1.3
1,023,689	-				-1.2
1,023,689	-				-0.5
1,023,698	-				-1.2
1,023,770	+				+1.0
1,023,770	+				+1.6
1,023,867	-				-0.8
1,023,867	-				+0.8
1,023,876	+				-0.2
1,023,937	-	helD	b0962	0.12	+0.5