Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhF and ybhG overlap by 8 nucleotides ybhG and ybiH overlap by 1 nucleotides ybiH and rhlE are separated by 228 nucleotides
b0794: ybhF - putative ATP-binding component of a transport system (VIMSS), at 826,468 to 828,204
ybhF
b0795: ybhG - hypothetical protein (NCBI), at 828,197 to 829,195
ybhG
b0796: ybiH - putative transcriptional regulator (VIMSS), at 829,195 to 829,866
ybiH
b0797: rhlE - RNA helicase (NCBI), at 830,095 to 831,459
rhlE
Position (kb) 828
829
830 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 827.246 kb on - strand, within ybhF at 827.246 kb on - strand, within ybhF at 827.256 kb on - strand, within ybhF at 827.256 kb on - strand, within ybhF at 827.256 kb on - strand, within ybhF at 827.288 kb on - strand, within ybhF at 827.288 kb on - strand, within ybhF at 827.464 kb on + strand, within ybhF at 827.470 kb on + strand, within ybhF at 827.547 kb on - strand, within ybhF at 827.549 kb on + strand, within ybhF at 827.549 kb on + strand, within ybhF at 827.554 kb on + strand, within ybhF at 827.881 kb on + strand, within ybhF at 827.906 kb on - strand, within ybhF at 827.949 kb on + strand, within ybhF at 828.002 kb on - strand, within ybhF at 828.002 kb on - strand, within ybhF at 828.069 kb on + strand at 828.073 kb on - strand at 828.130 kb on - strand at 828.143 kb on + strand at 828.156 kb on - strand at 828.197 kb on + strand at 828.197 kb on + strand at 828.205 kb on - strand at 828.207 kb on - strand at 828.311 kb on - strand, within ybhG at 828.410 kb on - strand, within ybhG at 828.469 kb on + strand, within ybhG at 828.502 kb on - strand, within ybhG at 828.504 kb on + strand, within ybhG at 828.528 kb on - strand, within ybhG at 828.528 kb on - strand, within ybhG at 828.620 kb on - strand, within ybhG at 828.725 kb on - strand, within ybhG at 828.876 kb on + strand, within ybhG at 828.906 kb on - strand, within ybhG at 828.949 kb on - strand, within ybhG at 828.953 kb on + strand, within ybhG at 828.959 kb on - strand, within ybhG at 828.959 kb on - strand, within ybhG at 828.978 kb on + strand, within ybhG at 828.978 kb on + strand, within ybhG at 829.047 kb on - strand at 829.088 kb on - strand, within ybhG at 829.093 kb on - strand, within ybhG at 829.295 kb on - strand, within ybiH at 829.360 kb on + strand, within ybiH at 829.360 kb on + strand, within ybiH at 829.535 kb on - strand, within ybiH at 829.598 kb on + strand, within ybiH at 829.679 kb on + strand, within ybiH at 829.841 kb on - strand at 829.841 kb on - strand at 829.845 kb on - strand at 829.858 kb on + strand at 829.996 kb on + strand at 830.051 kb on + strand at 830.059 kb on + strand at 830.059 kb on + strand at 830.123 kb on + strand at 830.123 kb on + strand at 830.123 kb on + strand at 830.146 kb on + strand at 830.156 kb on + strand at 830.159 kb on - strand at 830.176 kb on - strand at 830.182 kb on + strand at 830.182 kb on + strand at 830.192 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 827,246 - ybhF b0794 0.45 +0.9 827,246 - ybhF b0794 0.45 +0.0 827,256 - ybhF b0794 0.45 -0.7 827,256 - ybhF b0794 0.45 -2.3 827,256 - ybhF b0794 0.45 +1.1 827,288 - ybhF b0794 0.47 -1.6 827,288 - ybhF b0794 0.47 -3.0 827,464 + ybhF b0794 0.57 +0.5 827,470 + ybhF b0794 0.58 +0.8 827,547 - ybhF b0794 0.62 -0.4 827,549 + ybhF b0794 0.62 -1.9 827,549 + ybhF b0794 0.62 -0.1 827,554 + ybhF b0794 0.63 +0.0 827,881 + ybhF b0794 0.81 +0.4 827,906 - ybhF b0794 0.83 -1.1 827,949 + ybhF b0794 0.85 -0.4 828,002 - ybhF b0794 0.88 -1.4 828,002 - ybhF b0794 0.88 -1.1 828,069 + -1.3 828,073 - +1.1 828,130 - -3.6 828,143 + -0.1 828,156 - -0.4 828,197 + -1.2 828,197 + -0.7 828,205 - +0.7 828,207 - -0.0 828,311 - ybhG b0795 0.11 -1.3 828,410 - ybhG b0795 0.21 +0.4 828,469 + ybhG b0795 0.27 -0.8 828,502 - ybhG b0795 0.31 -0.1 828,504 + ybhG b0795 0.31 +0.4 828,528 - ybhG b0795 0.33 -0.8 828,528 - ybhG b0795 0.33 -0.8 828,620 - ybhG b0795 0.42 -0.8 828,725 - ybhG b0795 0.53 -0.6 828,876 + ybhG b0795 0.68 -0.5 828,906 - ybhG b0795 0.71 +0.7 828,949 - ybhG b0795 0.75 -0.7 828,953 + ybhG b0795 0.76 +0.9 828,959 - ybhG b0795 0.76 -0.0 828,959 - ybhG b0795 0.76 -1.1 828,978 + ybhG b0795 0.78 -0.7 828,978 + ybhG b0795 0.78 -2.1 829,047 - +1.9 829,088 - ybhG b0795 0.89 -0.6 829,093 - ybhG b0795 0.90 +0.4 829,295 - ybiH b0796 0.15 +0.7 829,360 + ybiH b0796 0.25 -0.1 829,360 + ybiH b0796 0.25 -0.7 829,535 - ybiH b0796 0.51 +0.8 829,598 + ybiH b0796 0.60 -1.3 829,679 + ybiH b0796 0.72 +1.8 829,841 - -1.3 829,841 - -0.1 829,845 - +0.3 829,858 + -1.4 829,996 + -1.9 830,051 + -0.7 830,059 + +0.3 830,059 + -1.1 830,123 + +1.3 830,123 + -0.5 830,123 + +0.3 830,146 + +0.6 830,156 + +2.0 830,159 - +0.8 830,176 - -0.6 830,182 + +0.8 830,182 + +3.2 830,192 - +0.3
Or see this region's nucleotide sequence