Strain Fitness in Escherichia coli BW25113 around b0714

Experiment: LB Aerobic with Irgasan 0.0000003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybgK and ybgL overlap by 11 nucleotidesybgL and nei are separated by 35 nucleotidesnei and abrB overlap by 4 nucleotides b0712: ybgK - predicted enzyme subunit (NCBI), at 743,466 to 744,398 ybgK b0713: ybgL - hypothetical protein (NCBI), at 744,388 to 745,122 ybgL b0714: nei - endonuclease VIII/ 5-formyluracil/5-hydroxymethyluracil DNA glycosylase (NCBI), at 745,158 to 745,949 nei b0715: abrB - putative transport protein (VIMSS), at 745,946 to 746,992 abrB Position (kb) 745 746Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 744.271 kb on - strand, within ybgKat 744.423 kb on - strandat 744.662 kb on - strand, within ybgLat 744.662 kb on - strand, within ybgLat 744.711 kb on + strand, within ybgLat 744.766 kb on + strand, within ybgLat 744.832 kb on - strand, within ybgLat 744.978 kb on - strand, within ybgLat 744.978 kb on - strand, within ybgLat 745.140 kb on - strandat 745.148 kb on + strandat 745.194 kb on + strandat 745.194 kb on + strandat 745.286 kb on + strand, within neiat 745.297 kb on + strand, within neiat 745.297 kb on + strand, within neiat 745.358 kb on - strand, within neiat 745.358 kb on - strand, within neiat 745.419 kb on + strand, within neiat 745.689 kb on - strand, within neiat 745.991 kb on + strandat 746.028 kb on - strandat 746.035 kb on - strandat 746.088 kb on + strand, within abrBat 746.122 kb on - strand, within abrBat 746.218 kb on - strand, within abrBat 746.267 kb on - strand, within abrBat 746.335 kb on - strand, within abrBat 746.335 kb on - strand, within abrBat 746.412 kb on - strand, within abrBat 746.422 kb on - strand, within abrBat 746.509 kb on + strand, within abrBat 746.651 kb on - strand, within abrBat 746.768 kb on + strand, within abrBat 746.768 kb on + strand, within abrBat 746.815 kb on - strand, within abrBat 746.869 kb on + strand, within abrBat 746.929 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM
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744,271 - ybgK b0712 0.86 -0.8
744,423 - -1.5
744,662 - ybgL b0713 0.37 +1.9
744,662 - ybgL b0713 0.37 +1.6
744,711 + ybgL b0713 0.44 +0.7
744,766 + ybgL b0713 0.51 -0.8
744,832 - ybgL b0713 0.60 +0.2
744,978 - ybgL b0713 0.80 -0.1
744,978 - ybgL b0713 0.80 -2.0
745,140 - -1.0
745,148 + -0.6
745,194 + -0.3
745,194 + -0.6
745,286 + nei b0714 0.16 -2.0
745,297 + nei b0714 0.18 +0.5
745,297 + nei b0714 0.18 -0.9
745,358 - nei b0714 0.25 -0.2
745,358 - nei b0714 0.25 -2.8
745,419 + nei b0714 0.33 +0.4
745,689 - nei b0714 0.67 +1.3
745,991 + -0.0
746,028 - +1.3
746,035 - -1.0
746,088 + abrB b0715 0.14 +0.2
746,122 - abrB b0715 0.17 -0.7
746,218 - abrB b0715 0.26 +1.0
746,267 - abrB b0715 0.31 +0.3
746,335 - abrB b0715 0.37 -1.3
746,335 - abrB b0715 0.37 +0.4
746,412 - abrB b0715 0.45 +1.6
746,422 - abrB b0715 0.45 -0.1
746,509 + abrB b0715 0.54 +1.2
746,651 - abrB b0715 0.67 +0.3
746,768 + abrB b0715 0.79 -0.8
746,768 + abrB b0715 0.79 +0.2
746,815 - abrB b0715 0.83 +1.1
746,869 + abrB b0715 0.88 -0.5
746,929 - +1.5

Or see this region's nucleotide sequence