Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeT and ybeU are separated by 101 nucleotides ybeU and djlC overlap by 4 nucleotides djlC and hscC are separated by 59 nucleotides
b0647: ybeT - conserved outer membrane protein (NCBI), at 678,075 to 678,629
ybeT
b0648: ybeU - predicted tRNA ligase (NCBI), at 678,731 to 679,438
ybeU
b0649: djlC - Hsc56 co-chaperone of HscC (NCBI), at 679,435 to 680,886
djlC
b0650: hscC - Hsp70 family chaperone Hsc62, binds to RpoD and inhibits transcription (NCBI), at 680,946 to 682,616
hscC
Position (kb) 679
680
681 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 678.442 kb on + strand, within ybeT at 678.447 kb on + strand, within ybeT at 678.452 kb on + strand, within ybeT at 678.464 kb on + strand, within ybeT at 678.465 kb on + strand, within ybeT at 678.465 kb on + strand, within ybeT at 678.469 kb on + strand, within ybeT at 678.475 kb on + strand, within ybeT at 678.475 kb on + strand, within ybeT at 678.481 kb on + strand, within ybeT at 678.481 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.485 kb on + strand, within ybeT at 678.543 kb on + strand, within ybeT at 678.566 kb on + strand, within ybeT at 678.801 kb on - strand at 678.907 kb on - strand, within ybeU at 678.917 kb on - strand, within ybeU at 678.946 kb on + strand, within ybeU at 678.949 kb on - strand, within ybeU at 678.964 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.966 kb on - strand, within ybeU at 678.969 kb on + strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 678.977 kb on - strand, within ybeU at 679.009 kb on - strand, within ybeU at 679.014 kb on + strand, within ybeU at 679.045 kb on - strand, within ybeU at 679.051 kb on + strand, within ybeU at 679.058 kb on + strand, within ybeU at 679.059 kb on - strand, within ybeU at 679.059 kb on - strand, within ybeU at 679.072 kb on - strand, within ybeU at 679.113 kb on - strand, within ybeU at 679.121 kb on + strand, within ybeU at 679.243 kb on - strand, within ybeU at 679.293 kb on - strand, within ybeU at 679.293 kb on - strand, within ybeU at 679.350 kb on + strand, within ybeU at 679.381 kb on + strand at 679.414 kb on + strand at 679.414 kb on + strand at 679.415 kb on + strand at 679.415 kb on + strand at 679.538 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.547 kb on + strand at 679.569 kb on - strand at 679.571 kb on + strand at 679.574 kb on - strand at 679.576 kb on - strand at 679.785 kb on - strand, within djlC at 679.788 kb on + strand, within djlC at 679.828 kb on - strand, within djlC at 679.964 kb on - strand, within djlC at 679.964 kb on - strand, within djlC at 679.979 kb on - strand, within djlC at 680.012 kb on + strand, within djlC at 680.020 kb on - strand, within djlC at 680.034 kb on - strand, within djlC at 680.034 kb on - strand, within djlC at 680.136 kb on - strand, within djlC at 680.160 kb on - strand, within djlC at 680.196 kb on + strand, within djlC at 680.196 kb on + strand, within djlC at 680.234 kb on + strand, within djlC at 680.234 kb on + strand, within djlC at 680.580 kb on - strand, within djlC at 680.632 kb on + strand, within djlC at 680.632 kb on + strand, within djlC at 680.643 kb on + strand, within djlC at 680.643 kb on + strand, within djlC at 680.841 kb on + strand at 680.857 kb on - strand at 680.936 kb on + strand at 681.182 kb on - strand, within hscC at 681.210 kb on + strand, within hscC at 681.214 kb on - strand, within hscC at 681.390 kb on + strand, within hscC at 681.390 kb on + strand, within hscC at 681.466 kb on - strand, within hscC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 678,442 + ybeT b0647 0.66 -0.6 678,447 + ybeT b0647 0.67 +0.7 678,452 + ybeT b0647 0.68 -1.7 678,464 + ybeT b0647 0.70 -0.0 678,465 + ybeT b0647 0.70 -0.6 678,465 + ybeT b0647 0.70 +1.0 678,469 + ybeT b0647 0.71 -1.3 678,475 + ybeT b0647 0.72 -1.3 678,475 + ybeT b0647 0.72 +0.7 678,481 + ybeT b0647 0.73 +1.0 678,481 + ybeT b0647 0.73 -0.0 678,485 + ybeT b0647 0.74 +1.0 678,485 + ybeT b0647 0.74 +1.0 678,485 + ybeT b0647 0.74 -0.0 678,485 + ybeT b0647 0.74 +1.0 678,485 + ybeT b0647 0.74 +1.0 678,543 + ybeT b0647 0.84 +1.0 678,566 + ybeT b0647 0.88 +1.0 678,801 - +1.0 678,907 - ybeU b0648 0.25 -0.0 678,917 - ybeU b0648 0.26 +1.0 678,946 + ybeU b0648 0.30 +1.0 678,949 - ybeU b0648 0.31 -0.0 678,964 - ybeU b0648 0.33 -0.0 678,966 - ybeU b0648 0.33 -0.0 678,966 - ybeU b0648 0.33 +1.0 678,966 - ybeU b0648 0.33 -1.0 678,966 - ybeU b0648 0.33 -1.4 678,966 - ybeU b0648 0.33 +3.0 678,969 + ybeU b0648 0.34 +1.0 678,977 - ybeU b0648 0.35 -0.0 678,977 - ybeU b0648 0.35 -0.0 678,977 - ybeU b0648 0.35 -0.0 678,977 - ybeU b0648 0.35 +2.6 679,009 - ybeU b0648 0.39 -1.0 679,014 + ybeU b0648 0.40 -0.0 679,045 - ybeU b0648 0.44 +1.0 679,051 + ybeU b0648 0.45 +0.7 679,058 + ybeU b0648 0.46 +1.8 679,059 - ybeU b0648 0.46 -0.0 679,059 - ybeU b0648 0.46 -2.7 679,072 - ybeU b0648 0.48 -1.0 679,113 - ybeU b0648 0.54 -1.0 679,121 + ybeU b0648 0.55 -0.0 679,243 - ybeU b0648 0.72 -1.0 679,293 - ybeU b0648 0.79 -1.6 679,293 - ybeU b0648 0.79 +0.4 679,350 + ybeU b0648 0.87 +0.4 679,381 + -1.6 679,414 + -0.4 679,414 + -1.2 679,415 + +1.0 679,415 + +1.0 679,538 + -2.2 679,547 + +0.7 679,547 + -1.0 679,547 + -1.1 679,547 + -0.0 679,569 - +1.9 679,571 + -0.0 679,574 - -0.3 679,576 - -1.2 679,785 - djlC b0649 0.24 +0.3 679,788 + djlC b0649 0.24 -0.5 679,828 - djlC b0649 0.27 -2.7 679,964 - djlC b0649 0.36 -0.0 679,964 - djlC b0649 0.36 +1.0 679,979 - djlC b0649 0.37 -0.0 680,012 + djlC b0649 0.40 +0.1 680,020 - djlC b0649 0.40 -1.0 680,034 - djlC b0649 0.41 +1.0 680,034 - djlC b0649 0.41 -1.0 680,136 - djlC b0649 0.48 +1.0 680,160 - djlC b0649 0.50 -2.2 680,196 + djlC b0649 0.52 -0.6 680,196 + djlC b0649 0.52 +1.0 680,234 + djlC b0649 0.55 +0.7 680,234 + djlC b0649 0.55 +0.2 680,580 - djlC b0649 0.79 -1.3 680,632 + djlC b0649 0.82 -0.6 680,632 + djlC b0649 0.82 -0.6 680,643 + djlC b0649 0.83 +1.0 680,643 + djlC b0649 0.83 -2.2 680,841 + +0.6 680,857 - -0.4 680,936 + +1.0 681,182 - hscC b0650 0.14 -0.1 681,210 + hscC b0650 0.16 -1.0 681,214 - hscC b0650 0.16 -3.0 681,390 + hscC b0650 0.27 +1.0 681,390 + hscC b0650 0.27 +1.0 681,466 - hscC b0650 0.31 +0.1
Or see this region's nucleotide sequence