Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cstA and ybdD are separated by 182 nucleotides ybdD and ybdH are separated by 9 nucleotides ybdH and ybdL are separated by 108 nucleotides
b0598: cstA - carbon starvation protein (NCBI), at 629,117 to 631,222
cstA
b4512: ybdD - hypothetical protein (NCBI), at 631,405 to 631,602
ybdD
b0599: ybdH - predicted oxidoreductase (NCBI), at 631,612 to 632,700
ybdH
b0600: ybdL - putative aminotransferase (NCBI), at 632,809 to 633,969
ybdL
Position (kb) 631
632
633 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 630.640 kb on + strand, within cstA at 630.703 kb on + strand, within cstA at 630.766 kb on + strand, within cstA at 630.816 kb on + strand, within cstA at 630.834 kb on - strand, within cstA at 630.834 kb on - strand, within cstA at 630.853 kb on - strand, within cstA at 630.865 kb on + strand, within cstA at 630.865 kb on + strand, within cstA at 630.894 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 630.927 kb on - strand, within cstA at 631.000 kb on + strand, within cstA at 631.000 kb on + strand, within cstA at 631.003 kb on + strand, within cstA at 631.068 kb on + strand at 631.068 kb on + strand at 631.126 kb on + strand at 631.180 kb on - strand at 631.194 kb on + strand at 631.194 kb on + strand at 631.236 kb on - strand at 631.250 kb on + strand at 631.262 kb on + strand at 631.262 kb on + strand at 631.264 kb on + strand at 631.278 kb on - strand at 631.300 kb on - strand at 631.300 kb on - strand at 631.305 kb on + strand at 631.398 kb on + strand at 631.398 kb on + strand at 631.401 kb on - strand at 631.413 kb on - strand at 631.413 kb on - strand at 631.780 kb on - strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.782 kb on + strand, within ybdH at 631.825 kb on + strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.828 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.833 kb on - strand, within ybdH at 631.926 kb on + strand, within ybdH at 631.926 kb on - strand, within ybdH at 632.035 kb on + strand, within ybdH at 632.048 kb on + strand, within ybdH at 632.147 kb on + strand, within ybdH at 632.160 kb on + strand, within ybdH at 632.399 kb on + strand, within ybdH at 632.466 kb on - strand, within ybdH at 632.581 kb on + strand, within ybdH at 632.877 kb on - strand at 632.877 kb on - strand at 632.948 kb on + strand, within ybdL at 633.223 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on + strand, within ybdL at 633.228 kb on - strand, within ybdL at 633.320 kb on - strand, within ybdL at 633.322 kb on - strand, within ybdL at 633.327 kb on - strand, within ybdL at 633.351 kb on - strand, within ybdL at 633.418 kb on + strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.418 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.442 kb on - strand, within ybdL at 633.541 kb on - strand, within ybdL at 633.551 kb on - strand, within ybdL at 633.570 kb on - strand, within ybdL at 633.572 kb on + strand, within ybdL at 633.587 kb on + strand, within ybdL at 633.597 kb on - strand, within ybdL
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 630,640 + cstA b0598 0.72 +0.6 630,703 + cstA b0598 0.75 -0.9 630,766 + cstA b0598 0.78 -1.3 630,816 + cstA b0598 0.81 +0.3 630,834 - cstA b0598 0.82 +0.8 630,834 - cstA b0598 0.82 +1.2 630,853 - cstA b0598 0.82 +0.1 630,865 + cstA b0598 0.83 -0.4 630,865 + cstA b0598 0.83 -0.9 630,894 - cstA b0598 0.84 +0.6 630,927 - cstA b0598 0.86 +1.3 630,927 - cstA b0598 0.86 -1.4 631,000 + cstA b0598 0.89 -1.4 631,000 + cstA b0598 0.89 +0.3 631,003 + cstA b0598 0.90 -0.5 631,068 + +1.0 631,068 + -0.0 631,126 + -1.8 631,180 - +2.5 631,194 + -0.1 631,194 + -0.9 631,236 - -0.2 631,250 + -0.7 631,262 + -0.1 631,262 + +2.0 631,264 + -0.4 631,278 - +0.0 631,300 - +0.4 631,300 - +0.9 631,305 + -2.0 631,398 + -1.5 631,398 + -1.5 631,401 - -1.3 631,413 - -1.2 631,413 - +0.0 631,780 - ybdH b0599 0.15 -0.2 631,782 + ybdH b0599 0.16 -1.5 631,782 + ybdH b0599 0.16 +0.3 631,825 + ybdH b0599 0.20 -0.1 631,828 - ybdH b0599 0.20 +0.8 631,828 - ybdH b0599 0.20 +1.1 631,833 - ybdH b0599 0.20 -1.0 631,833 - ybdH b0599 0.20 +1.4 631,926 + ybdH b0599 0.29 +0.5 631,926 - ybdH b0599 0.29 +0.2 632,035 + ybdH b0599 0.39 -0.5 632,048 + ybdH b0599 0.40 +0.0 632,147 + ybdH b0599 0.49 +1.0 632,160 + ybdH b0599 0.50 +2.1 632,399 + ybdH b0599 0.72 -0.9 632,466 - ybdH b0599 0.78 -1.3 632,581 + ybdH b0599 0.89 +0.8 632,877 - -1.3 632,877 - -1.8 632,948 + ybdL b0600 0.12 +1.4 633,223 + ybdL b0600 0.36 -1.5 633,228 + ybdL b0600 0.36 +2.2 633,228 + ybdL b0600 0.36 -1.3 633,228 - ybdL b0600 0.36 -1.1 633,320 - ybdL b0600 0.44 +1.0 633,322 - ybdL b0600 0.44 +0.4 633,327 - ybdL b0600 0.45 -2.8 633,351 - ybdL b0600 0.47 +0.9 633,418 + ybdL b0600 0.52 +0.6 633,418 - ybdL b0600 0.52 -0.7 633,418 - ybdL b0600 0.52 +0.0 633,442 - ybdL b0600 0.55 -0.8 633,442 - ybdL b0600 0.55 -2.8 633,541 - ybdL b0600 0.63 -0.5 633,551 - ybdL b0600 0.64 +0.6 633,570 - ybdL b0600 0.66 -0.6 633,572 + ybdL b0600 0.66 -0.0 633,587 + ybdL b0600 0.67 -0.7 633,597 - ybdL b0600 0.68 +0.1
Or see this region's nucleotide sequence