Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt allB and ybbY are separated by 56 nucleotides ybbY and glxK are separated by 21 nucleotides
b0512: allB - allantoinase (NCBI), at 538,371 to 539,732
allB
b0513: ybbY - putative transport (VIMSS), at 539,789 to 541,090
ybbY
b0514: glxK - glycerate kinase II (NCBI), at 541,112 to 542,257
glxK
Position (kb) 539
540
541
542 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 538.815 kb on + strand, within allB at 538.828 kb on - strand, within allB at 538.920 kb on + strand, within allB at 538.925 kb on - strand, within allB at 539.002 kb on + strand, within allB at 539.002 kb on + strand, within allB at 539.008 kb on - strand, within allB at 539.112 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.145 kb on + strand, within allB at 539.180 kb on + strand, within allB at 539.328 kb on - strand, within allB at 539.351 kb on + strand, within allB at 539.351 kb on + strand, within allB at 539.351 kb on + strand, within allB at 539.364 kb on - strand, within allB at 539.397 kb on + strand, within allB at 539.622 kb on - strand at 539.686 kb on + strand at 539.731 kb on + strand at 539.739 kb on - strand at 539.951 kb on + strand, within ybbY at 539.964 kb on - strand, within ybbY at 539.964 kb on - strand, within ybbY at 539.966 kb on + strand, within ybbY at 539.969 kb on - strand, within ybbY at 539.969 kb on - strand, within ybbY at 539.996 kb on + strand, within ybbY at 540.014 kb on - strand, within ybbY at 540.014 kb on - strand, within ybbY at 540.105 kb on + strand, within ybbY at 540.149 kb on + strand, within ybbY at 540.315 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.325 kb on + strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.404 kb on - strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.446 kb on + strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.455 kb on - strand, within ybbY at 540.484 kb on - strand, within ybbY at 540.502 kb on + strand, within ybbY at 540.526 kb on + strand, within ybbY at 540.531 kb on - strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.554 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.593 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.607 kb on + strand, within ybbY at 540.612 kb on + strand, within ybbY at 540.613 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY at 540.622 kb on + strand, within ybbY at 540.784 kb on + strand, within ybbY at 540.787 kb on - strand, within ybbY at 540.792 kb on - strand, within ybbY at 540.803 kb on + strand, within ybbY at 540.804 kb on - strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.805 kb on + strand, within ybbY at 540.813 kb on - strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.817 kb on + strand, within ybbY at 540.824 kb on + strand, within ybbY at 540.825 kb on - strand, within ybbY at 540.829 kb on + strand, within ybbY at 540.831 kb on + strand, within ybbY at 540.834 kb on + strand, within ybbY at 540.854 kb on + strand, within ybbY at 540.854 kb on + strand, within ybbY at 540.860 kb on + strand, within ybbY at 540.880 kb on + strand, within ybbY at 540.880 kb on + strand, within ybbY at 540.882 kb on + strand, within ybbY at 540.917 kb on + strand, within ybbY at 540.967 kb on + strand at 540.971 kb on + strand at 540.976 kb on + strand at 540.980 kb on - strand at 540.987 kb on + strand at 540.987 kb on + strand at 541.001 kb on + strand at 541.001 kb on + strand at 541.001 kb on - strand at 541.005 kb on + strand at 541.007 kb on + strand at 541.007 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.010 kb on + strand at 541.012 kb on + strand at 541.012 kb on + strand at 541.018 kb on - strand at 541.018 kb on - strand at 541.020 kb on - strand at 541.020 kb on - strand at 541.023 kb on + strand at 541.023 kb on + strand at 541.029 kb on - strand at 541.035 kb on + strand at 541.062 kb on + strand at 541.062 kb on + strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.070 kb on - strand at 541.125 kb on - strand at 541.135 kb on - strand at 541.155 kb on + strand at 541.177 kb on + strand at 541.177 kb on + strand at 541.266 kb on - strand, within glxK at 541.269 kb on - strand, within glxK at 541.295 kb on - strand, within glxK at 541.296 kb on + strand, within glxK at 541.432 kb on - strand, within glxK at 541.452 kb on - strand, within glxK at 541.452 kb on - strand, within glxK at 541.472 kb on + strand, within glxK at 541.502 kb on - strand, within glxK at 541.545 kb on + strand, within glxK at 541.547 kb on + strand, within glxK at 541.560 kb on + strand, within glxK at 541.560 kb on + strand, within glxK at 541.637 kb on - strand, within glxK at 541.644 kb on + strand, within glxK at 541.719 kb on + strand, within glxK at 541.740 kb on - strand, within glxK at 541.866 kb on + strand, within glxK at 541.875 kb on + strand, within glxK at 541.886 kb on + strand, within glxK at 541.960 kb on - strand, within glxK at 541.968 kb on - strand, within glxK at 542.031 kb on + strand, within glxK at 542.031 kb on + strand, within glxK at 542.031 kb on + strand, within glxK at 542.039 kb on - strand, within glxK at 542.043 kb on - strand, within glxK at 542.053 kb on + strand, within glxK at 542.053 kb on + strand, within glxK at 542.070 kb on + strand, within glxK at 542.073 kb on + strand, within glxK at 542.086 kb on + strand, within glxK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 538,815 + allB b0512 0.33 +1.0 538,828 - allB b0512 0.34 +0.2 538,920 + allB b0512 0.40 +1.0 538,925 - allB b0512 0.41 -0.5 539,002 + allB b0512 0.46 +2.1 539,002 + allB b0512 0.46 -0.9 539,008 - allB b0512 0.47 +0.3 539,112 + allB b0512 0.54 -0.7 539,145 + allB b0512 0.57 -0.7 539,145 + allB b0512 0.57 +0.0 539,145 + allB b0512 0.57 -1.2 539,180 + allB b0512 0.59 -0.2 539,328 - allB b0512 0.70 +0.1 539,351 + allB b0512 0.72 -0.9 539,351 + allB b0512 0.72 -0.2 539,351 + allB b0512 0.72 -1.0 539,364 - allB b0512 0.73 -1.2 539,397 + allB b0512 0.75 +0.5 539,622 - +0.6 539,686 + -1.2 539,731 + +1.0 539,739 - -1.0 539,951 + ybbY b0513 0.12 -0.9 539,964 - ybbY b0513 0.13 +1.1 539,964 - ybbY b0513 0.13 +0.5 539,966 + ybbY b0513 0.14 -0.5 539,969 - ybbY b0513 0.14 +1.5 539,969 - ybbY b0513 0.14 +1.1 539,996 + ybbY b0513 0.16 -1.8 540,014 - ybbY b0513 0.17 +1.1 540,014 - ybbY b0513 0.17 +1.1 540,105 + ybbY b0513 0.24 -0.5 540,149 + ybbY b0513 0.28 -0.2 540,315 + ybbY b0513 0.40 -0.1 540,325 + ybbY b0513 0.41 +1.7 540,325 + ybbY b0513 0.41 +2.1 540,404 - ybbY b0513 0.47 +2.1 540,404 - ybbY b0513 0.47 +1.1 540,446 + ybbY b0513 0.50 +1.1 540,446 + ybbY b0513 0.50 +0.3 540,455 - ybbY b0513 0.51 +1.7 540,455 - ybbY b0513 0.51 -0.4 540,484 - ybbY b0513 0.53 -2.4 540,502 + ybbY b0513 0.55 -1.5 540,526 + ybbY b0513 0.57 -0.5 540,531 - ybbY b0513 0.57 +1.1 540,554 + ybbY b0513 0.59 -0.9 540,554 + ybbY b0513 0.59 -0.9 540,593 + ybbY b0513 0.62 +0.8 540,593 + ybbY b0513 0.62 -1.5 540,607 + ybbY b0513 0.63 -0.9 540,607 + ybbY b0513 0.63 +0.6 540,607 + ybbY b0513 0.63 -1.1 540,607 + ybbY b0513 0.63 -2.3 540,612 + ybbY b0513 0.63 -0.0 540,613 + ybbY b0513 0.63 -0.5 540,622 + ybbY b0513 0.64 +1.6 540,622 + ybbY b0513 0.64 -2.0 540,784 + ybbY b0513 0.76 +1.6 540,787 - ybbY b0513 0.77 -1.2 540,792 - ybbY b0513 0.77 +1.1 540,803 + ybbY b0513 0.78 +2.3 540,804 - ybbY b0513 0.78 +1.1 540,805 + ybbY b0513 0.78 +1.1 540,805 + ybbY b0513 0.78 +0.9 540,805 + ybbY b0513 0.78 +2.3 540,805 + ybbY b0513 0.78 +0.6 540,805 + ybbY b0513 0.78 +1.2 540,813 - ybbY b0513 0.79 +1.1 540,817 + ybbY b0513 0.79 +2.4 540,817 + ybbY b0513 0.79 +0.2 540,817 + ybbY b0513 0.79 -1.2 540,817 + ybbY b0513 0.79 -0.5 540,817 + ybbY b0513 0.79 -0.5 540,817 + ybbY b0513 0.79 -0.5 540,824 + ybbY b0513 0.79 -1.9 540,825 - ybbY b0513 0.80 +1.1 540,829 + ybbY b0513 0.80 +1.3 540,831 + ybbY b0513 0.80 -1.0 540,834 + ybbY b0513 0.80 -1.2 540,854 + ybbY b0513 0.82 +0.1 540,854 + ybbY b0513 0.82 -0.2 540,860 + ybbY b0513 0.82 +0.4 540,880 + ybbY b0513 0.84 -1.5 540,880 + ybbY b0513 0.84 +0.3 540,882 + ybbY b0513 0.84 +0.3 540,917 + ybbY b0513 0.87 +1.1 540,967 + +0.7 540,971 + +3.1 540,976 + -0.5 540,980 - -1.2 540,987 + +0.1 540,987 + -0.5 541,001 + -2.1 541,001 + -1.2 541,001 - +2.1 541,005 + +0.4 541,007 + -0.5 541,007 + -0.3 541,010 + +0.2 541,010 + +0.1 541,010 + +0.7 541,010 + +0.5 541,012 + -0.4 541,012 + +0.0 541,018 - -0.3 541,018 - +2.8 541,020 - +0.1 541,020 - +0.1 541,023 + -0.9 541,023 + -1.7 541,029 - +1.1 541,035 + +0.7 541,062 + -0.1 541,062 + +1.5 541,070 - +0.1 541,070 - +3.1 541,070 - +1.1 541,070 - +6.1 541,125 - +2.1 541,135 - +0.3 541,155 + +1.1 541,177 + -0.5 541,177 + +1.1 541,266 - glxK b0514 0.13 +1.7 541,269 - glxK b0514 0.14 -3.2 541,295 - glxK b0514 0.16 +0.1 541,296 + glxK b0514 0.16 -0.5 541,432 - glxK b0514 0.28 +0.8 541,452 - glxK b0514 0.30 +0.1 541,452 - glxK b0514 0.30 +0.1 541,472 + glxK b0514 0.31 +0.3 541,502 - glxK b0514 0.34 -1.6 541,545 + glxK b0514 0.38 +0.3 541,547 + glxK b0514 0.38 -1.4 541,560 + glxK b0514 0.39 -0.9 541,560 + glxK b0514 0.39 -0.2 541,637 - glxK b0514 0.46 -0.2 541,644 + glxK b0514 0.46 -0.1 541,719 + glxK b0514 0.53 +0.9 541,740 - glxK b0514 0.55 -0.9 541,866 + glxK b0514 0.66 -1.9 541,875 + glxK b0514 0.67 +0.4 541,886 + glxK b0514 0.68 -0.2 541,960 - glxK b0514 0.74 -0.5 541,968 - glxK b0514 0.75 +1.5 542,031 + glxK b0514 0.80 -0.1 542,031 + glxK b0514 0.80 +0.2 542,031 + glxK b0514 0.80 +0.1 542,039 - glxK b0514 0.81 +2.1 542,043 - glxK b0514 0.81 -1.5 542,053 + glxK b0514 0.82 -0.8 542,053 + glxK b0514 0.82 -1.2 542,070 + glxK b0514 0.84 -1.8 542,073 + glxK b0514 0.84 +0.2 542,086 + glxK b0514 0.85 -3.0
Or see this region's nucleotide sequence