Experiment: LB Aerobic with Irgasan 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpT and yaiL are separated by 101 nucleotides yaiL and frmB are separated by 223 nucleotides frmB and frmA are separated by 93 nucleotides
b0353: mhpT - putative transport protein (VIMSS), at 374,683 to 375,894
mhpT
b0354: yaiL - nucleoprotein/polynucleotide-associated enzyme (VIMSS), at 375,996 to 376,535
yaiL
b0355: frmB - predicted esterase (NCBI), at 376,759 to 377,592
frmB
b0356: frmA - alcohol dehydrogenase class III/glutathione-dependent formaldehyde dehydrogenase (NCBI), at 377,686 to 378,795
frmA
Position (kb) 376
377
378 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 375.780 kb on - strand at 375.780 kb on - strand at 375.806 kb on + strand at 375.898 kb on + strand at 375.920 kb on - strand at 375.921 kb on - strand at 375.921 kb on - strand at 375.940 kb on - strand at 375.940 kb on - strand at 376.196 kb on + strand, within yaiL at 376.196 kb on + strand, within yaiL at 376.199 kb on + strand, within yaiL at 376.199 kb on + strand, within yaiL at 376.312 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.331 kb on + strand, within yaiL at 376.336 kb on - strand, within yaiL at 376.336 kb on - strand, within yaiL at 376.366 kb on - strand, within yaiL at 376.366 kb on - strand, within yaiL at 376.376 kb on - strand, within yaiL at 376.376 kb on - strand, within yaiL at 376.384 kb on - strand, within yaiL at 376.406 kb on - strand, within yaiL at 376.425 kb on + strand, within yaiL at 376.449 kb on + strand, within yaiL at 376.449 kb on - strand, within yaiL at 376.457 kb on - strand, within yaiL at 376.481 kb on + strand, within yaiL at 376.489 kb on - strand at 376.489 kb on - strand at 376.489 kb on - strand at 376.539 kb on + strand at 376.539 kb on - strand at 376.558 kb on + strand at 376.618 kb on - strand at 376.653 kb on + strand at 376.682 kb on + strand at 376.684 kb on + strand at 376.684 kb on + strand at 376.692 kb on - strand at 376.719 kb on + strand at 376.745 kb on - strand at 376.745 kb on - strand at 376.747 kb on + strand at 376.755 kb on + strand at 376.771 kb on - strand at 376.783 kb on + strand at 376.853 kb on - strand, within frmB at 376.875 kb on + strand, within frmB at 376.906 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.909 kb on + strand, within frmB at 376.927 kb on - strand, within frmB at 376.946 kb on - strand, within frmB at 377.044 kb on + strand, within frmB at 377.044 kb on + strand, within frmB at 377.052 kb on - strand, within frmB at 377.075 kb on + strand, within frmB at 377.075 kb on + strand, within frmB at 377.120 kb on - strand, within frmB at 377.120 kb on - strand, within frmB at 377.122 kb on + strand, within frmB at 377.375 kb on - strand, within frmB at 377.395 kb on - strand, within frmB at 377.400 kb on - strand, within frmB at 377.447 kb on + strand, within frmB at 377.499 kb on - strand, within frmB at 377.525 kb on + strand at 377.662 kb on - strand at 377.668 kb on + strand at 377.751 kb on + strand at 377.769 kb on - strand at 377.809 kb on + strand, within frmA at 377.859 kb on - strand, within frmA at 378.034 kb on - strand, within frmA at 378.051 kb on + strand, within frmA at 378.051 kb on + strand, within frmA at 378.135 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.256 kb on + strand, within frmA at 378.283 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.383 kb on - strand, within frmA at 378.449 kb on + strand, within frmA at 378.479 kb on - strand, within frmA at 378.479 kb on - strand, within frmA at 378.484 kb on - strand, within frmA at 378.523 kb on - strand, within frmA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Irgasan 0.0000003125 mM remove 375,780 - -1.6 375,780 - +1.2 375,806 + -0.8 375,898 + -0.1 375,920 - -1.9 375,921 - +2.2 375,921 - +1.2 375,940 - -2.4 375,940 - +0.8 376,196 + yaiL b0354 0.37 +1.5 376,196 + yaiL b0354 0.37 +1.3 376,199 + yaiL b0354 0.38 -0.6 376,199 + yaiL b0354 0.38 +0.2 376,312 + yaiL b0354 0.59 -0.5 376,331 + yaiL b0354 0.62 -0.1 376,331 + yaiL b0354 0.62 +0.9 376,331 + yaiL b0354 0.62 -0.8 376,336 - yaiL b0354 0.63 +0.0 376,336 - yaiL b0354 0.63 +0.2 376,366 - yaiL b0354 0.69 +1.3 376,366 - yaiL b0354 0.69 +0.6 376,376 - yaiL b0354 0.70 -1.8 376,376 - yaiL b0354 0.70 +1.0 376,384 - yaiL b0354 0.72 -0.4 376,406 - yaiL b0354 0.76 -0.3 376,425 + yaiL b0354 0.79 +0.2 376,449 + yaiL b0354 0.84 -0.0 376,449 - yaiL b0354 0.84 +0.1 376,457 - yaiL b0354 0.85 -1.6 376,481 + yaiL b0354 0.90 +1.2 376,489 - -2.4 376,489 - +0.6 376,489 - +0.9 376,539 + -0.1 376,539 - +0.7 376,558 + -0.1 376,618 - -0.5 376,653 + -1.6 376,682 + -1.3 376,684 + +0.4 376,684 + +0.5 376,692 - +0.2 376,719 + +1.1 376,745 - +0.2 376,745 - -1.2 376,747 + -0.8 376,755 + +0.8 376,771 - -0.6 376,783 + -1.8 376,853 - frmB b0355 0.11 +1.5 376,875 + frmB b0355 0.14 +2.0 376,906 + frmB b0355 0.18 +0.2 376,909 + frmB b0355 0.18 +2.0 376,909 + frmB b0355 0.18 -0.1 376,909 + frmB b0355 0.18 +2.2 376,927 - frmB b0355 0.20 +2.2 376,946 - frmB b0355 0.22 -0.1 377,044 + frmB b0355 0.34 +0.8 377,044 + frmB b0355 0.34 -2.1 377,052 - frmB b0355 0.35 -0.5 377,075 + frmB b0355 0.38 +1.5 377,075 + frmB b0355 0.38 +0.7 377,120 - frmB b0355 0.43 -1.3 377,120 - frmB b0355 0.43 +1.3 377,122 + frmB b0355 0.44 -1.6 377,375 - frmB b0355 0.74 +0.9 377,395 - frmB b0355 0.76 -0.2 377,400 - frmB b0355 0.77 +0.9 377,447 + frmB b0355 0.82 +0.2 377,499 - frmB b0355 0.89 -0.1 377,525 + +0.2 377,662 - -0.0 377,668 + -2.3 377,751 + -0.0 377,769 - -0.7 377,809 + frmA b0356 0.11 -1.8 377,859 - frmA b0356 0.16 -1.3 378,034 - frmA b0356 0.31 +0.3 378,051 + frmA b0356 0.33 -0.4 378,051 + frmA b0356 0.33 -0.1 378,135 + frmA b0356 0.40 +0.2 378,256 + frmA b0356 0.51 +0.9 378,256 + frmA b0356 0.51 -0.3 378,256 + frmA b0356 0.51 +0.4 378,283 - frmA b0356 0.54 +0.3 378,383 - frmA b0356 0.63 -3.5 378,383 - frmA b0356 0.63 +2.2 378,449 + frmA b0356 0.69 -0.4 378,479 - frmA b0356 0.71 -1.5 378,479 - frmA b0356 0.71 -1.6 378,484 - frmA b0356 0.72 -2.5 378,523 - frmA b0356 0.75 +0.2
Or see this region's nucleotide sequence