Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfaQ and kdtA are separated by 441 nucleotides kdtA and coaD are separated by 7 nucleotides coaD and mutM are separated by 38 nucleotides
b3632: rfaQ - lipopolysaccharide core biosynthesis protein (NCBI), at 3,805,087 to 3,806,121
rfaQ
b3633: kdtA - 3-deoxy-D-manno-octulosonic-acid transferase (NCBI), at 3,806,563 to 3,807,840
kdtA
b3634: coaD - phosphopantetheine adenylyltransferase (NCBI), at 3,807,848 to 3,808,327
coaD
b3635: mutM - formamidopyrimidine-DNA glycosylase (NCBI), at 3,808,366 to 3,809,175
mutM
Position (kb)
3806
3807
3808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3805.582 kb on - strand, within rfaQ at 3805.584 kb on + strand, within rfaQ at 3805.591 kb on - strand, within rfaQ at 3805.594 kb on - strand, within rfaQ at 3805.594 kb on - strand, within rfaQ at 3805.602 kb on - strand, within rfaQ at 3805.625 kb on - strand, within rfaQ at 3805.625 kb on - strand, within rfaQ at 3805.650 kb on - strand, within rfaQ at 3805.655 kb on - strand, within rfaQ at 3805.655 kb on - strand, within rfaQ at 3805.673 kb on + strand, within rfaQ at 3805.676 kb on + strand, within rfaQ at 3805.676 kb on + strand, within rfaQ at 3805.682 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.693 kb on - strand, within rfaQ at 3805.695 kb on + strand, within rfaQ at 3805.700 kb on - strand, within rfaQ at 3805.700 kb on - strand, within rfaQ at 3805.703 kb on - strand, within rfaQ at 3805.705 kb on - strand, within rfaQ at 3805.705 kb on - strand, within rfaQ at 3805.719 kb on - strand, within rfaQ at 3805.765 kb on + strand, within rfaQ at 3805.765 kb on + strand, within rfaQ at 3805.765 kb on - strand, within rfaQ at 3805.769 kb on - strand, within rfaQ at 3805.769 kb on - strand, within rfaQ at 3805.773 kb on - strand, within rfaQ at 3805.795 kb on - strand, within rfaQ at 3805.795 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.805 kb on - strand, within rfaQ at 3805.808 kb on - strand, within rfaQ at 3805.835 kb on + strand, within rfaQ at 3805.841 kb on - strand, within rfaQ at 3805.841 kb on - strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on + strand, within rfaQ at 3805.843 kb on - strand, within rfaQ at 3805.851 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.852 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.854 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.867 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.872 kb on - strand, within rfaQ at 3805.879 kb on + strand, within rfaQ at 3805.887 kb on - strand, within rfaQ at 3805.887 kb on - strand, within rfaQ at 3805.890 kb on + strand, within rfaQ at 3805.890 kb on + strand, within rfaQ at 3805.895 kb on + strand, within rfaQ at 3805.898 kb on - strand, within rfaQ at 3805.898 kb on - strand, within rfaQ at 3805.900 kb on - strand, within rfaQ at 3805.973 kb on + strand, within rfaQ at 3805.981 kb on - strand, within rfaQ at 3805.981 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3805.984 kb on - strand, within rfaQ at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.026 kb on - strand at 3806.033 kb on - strand at 3806.035 kb on - strand at 3806.038 kb on - strand at 3806.070 kb on - strand at 3806.070 kb on - strand at 3806.078 kb on - strand at 3806.083 kb on - strand at 3806.083 kb on - strand at 3806.088 kb on - strand at 3806.088 kb on - strand at 3806.096 kb on - strand at 3806.096 kb on - strand at 3806.096 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.101 kb on - strand at 3806.108 kb on - strand at 3806.162 kb on - strand at 3806.167 kb on - strand at 3806.177 kb on - strand at 3806.177 kb on - strand at 3806.222 kb on + strand at 3806.222 kb on + strand at 3806.241 kb on - strand at 3806.241 kb on - strand at 3806.263 kb on - strand at 3806.274 kb on - strand at 3806.274 kb on - strand at 3806.277 kb on - strand at 3806.381 kb on - strand at 3806.387 kb on - strand at 3806.560 kb on + strand at 3806.568 kb on - strand at 3806.568 kb on - strand at 3806.568 kb on - strand at 3806.574 kb on + strand at 3808.353 kb on - strand at 3808.433 kb on + strand at 3808.433 kb on + strand at 3808.443 kb on - strand at 3808.450 kb on - strand, within mutM at 3808.497 kb on + strand, within mutM at 3808.532 kb on + strand, within mutM at 3808.535 kb on - strand, within mutM at 3808.540 kb on - strand, within mutM at 3808.558 kb on - strand, within mutM at 3808.563 kb on - strand, within mutM at 3808.565 kb on + strand, within mutM at 3808.614 kb on - strand, within mutM at 3808.614 kb on - strand, within mutM at 3808.699 kb on - strand, within mutM at 3808.738 kb on + strand, within mutM at 3808.753 kb on - strand, within mutM at 3808.757 kb on + strand, within mutM at 3808.758 kb on - strand, within mutM at 3808.766 kb on - strand, within mutM at 3808.784 kb on + strand, within mutM at 3808.793 kb on - strand, within mutM at 3808.796 kb on - strand, within mutM at 3808.810 kb on - strand, within mutM at 3808.824 kb on - strand, within mutM at 3808.826 kb on + strand, within mutM at 3808.833 kb on + strand, within mutM at 3808.833 kb on + strand, within mutM at 3808.834 kb on - strand, within mutM at 3808.834 kb on - strand, within mutM
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM remove 3,805,582 - rfaQ b3632 0.48 -0.7 3,805,584 + rfaQ b3632 0.48 -0.1 3,805,591 - rfaQ b3632 0.49 +1.4 3,805,594 - rfaQ b3632 0.49 +1.2 3,805,594 - rfaQ b3632 0.49 -2.4 3,805,602 - rfaQ b3632 0.50 +0.1 3,805,625 - rfaQ b3632 0.52 -0.1 3,805,625 - rfaQ b3632 0.52 -0.4 3,805,650 - rfaQ b3632 0.54 -2.7 3,805,655 - rfaQ b3632 0.55 -0.1 3,805,655 - rfaQ b3632 0.55 +0.9 3,805,673 + rfaQ b3632 0.57 +1.4 3,805,676 + rfaQ b3632 0.57 -0.6 3,805,676 + rfaQ b3632 0.57 +0.4 3,805,682 - rfaQ b3632 0.57 +1.4 3,805,693 - rfaQ b3632 0.59 -1.6 3,805,693 - rfaQ b3632 0.59 +0.4 3,805,693 - rfaQ b3632 0.59 +0.1 3,805,693 - rfaQ b3632 0.59 +1.6 3,805,695 + rfaQ b3632 0.59 +0.4 3,805,700 - rfaQ b3632 0.59 -0.1 3,805,700 - rfaQ b3632 0.59 -0.6 3,805,703 - rfaQ b3632 0.60 -1.2 3,805,705 - rfaQ b3632 0.60 +2.7 3,805,705 - rfaQ b3632 0.60 -0.6 3,805,719 - rfaQ b3632 0.61 +1.2 3,805,765 + rfaQ b3632 0.66 +0.4 3,805,765 + rfaQ b3632 0.66 +1.4 3,805,765 - rfaQ b3632 0.66 -1.3 3,805,769 - rfaQ b3632 0.66 -2.0 3,805,769 - rfaQ b3632 0.66 -0.9 3,805,773 - rfaQ b3632 0.66 -2.0 3,805,795 - rfaQ b3632 0.68 -1.1 3,805,795 - rfaQ b3632 0.68 -0.1 3,805,805 - rfaQ b3632 0.69 -1.4 3,805,805 - rfaQ b3632 0.69 +1.1 3,805,805 - rfaQ b3632 0.69 -1.0 3,805,808 - rfaQ b3632 0.70 +0.1 3,805,835 + rfaQ b3632 0.72 +1.4 3,805,841 - rfaQ b3632 0.73 -2.1 3,805,841 - rfaQ b3632 0.73 -2.6 3,805,843 + rfaQ b3632 0.73 -0.1 3,805,843 + rfaQ b3632 0.73 +2.0 3,805,843 + rfaQ b3632 0.73 -0.6 3,805,843 - rfaQ b3632 0.73 -0.9 3,805,851 - rfaQ b3632 0.74 -1.3 3,805,852 - rfaQ b3632 0.74 -1.7 3,805,852 - rfaQ b3632 0.74 +1.4 3,805,852 - rfaQ b3632 0.74 +1.4 3,805,854 - rfaQ b3632 0.74 -1.4 3,805,854 - rfaQ b3632 0.74 +1.0 3,805,854 - rfaQ b3632 0.74 +1.0 3,805,854 - rfaQ b3632 0.74 -0.7 3,805,867 - rfaQ b3632 0.75 -1.7 3,805,867 - rfaQ b3632 0.75 -2.4 3,805,867 - rfaQ b3632 0.75 -1.0 3,805,872 - rfaQ b3632 0.76 -0.9 3,805,872 - rfaQ b3632 0.76 -2.5 3,805,872 - rfaQ b3632 0.76 -2.6 3,805,879 + rfaQ b3632 0.77 +1.4 3,805,887 - rfaQ b3632 0.77 +0.1 3,805,887 - rfaQ b3632 0.77 -0.1 3,805,890 + rfaQ b3632 0.78 +0.4 3,805,890 + rfaQ b3632 0.78 +1.4 3,805,895 + rfaQ b3632 0.78 +1.4 3,805,898 - rfaQ b3632 0.78 -1.4 3,805,898 - rfaQ b3632 0.78 -1.9 3,805,900 - rfaQ b3632 0.79 +1.4 3,805,973 + rfaQ b3632 0.86 +1.4 3,805,981 - rfaQ b3632 0.86 -2.6 3,805,981 - rfaQ b3632 0.86 -0.1 3,805,984 - rfaQ b3632 0.87 -0.9 3,805,984 - rfaQ b3632 0.87 -0.1 3,805,984 - rfaQ b3632 0.87 -1.0 3,806,026 - -1.0 3,806,026 - -1.3 3,806,026 - +0.1 3,806,026 - -2.1 3,806,033 - -0.5 3,806,035 - -0.9 3,806,038 - -2.7 3,806,070 - +0.4 3,806,070 - +0.4 3,806,078 - +0.2 3,806,083 - +0.9 3,806,083 - +0.4 3,806,088 - -0.2 3,806,088 - +0.1 3,806,096 - -0.5 3,806,096 - -3.3 3,806,096 - -0.5 3,806,101 - -0.3 3,806,101 - +2.0 3,806,101 - +0.4 3,806,101 - -0.6 3,806,108 - -0.1 3,806,162 - -1.9 3,806,167 - -0.6 3,806,177 - +1.3 3,806,177 - +0.0 3,806,222 + +0.4 3,806,222 + +1.4 3,806,241 - +0.3 3,806,241 - -0.8 3,806,263 - +0.8 3,806,274 - +0.3 3,806,274 - +0.6 3,806,277 - -0.4 3,806,381 - +0.4 3,806,387 - +1.0 3,806,560 + +0.2 3,806,568 - +0.2 3,806,568 - +1.3 3,806,568 - -0.0 3,806,574 + +0.3 3,808,353 - -1.8 3,808,433 + +3.0 3,808,433 + -1.5 3,808,443 - +0.6 3,808,450 - mutM b3635 0.10 -0.1 3,808,497 + mutM b3635 0.16 -1.1 3,808,532 + mutM b3635 0.20 +0.6 3,808,535 - mutM b3635 0.21 +1.0 3,808,540 - mutM b3635 0.21 +1.4 3,808,558 - mutM b3635 0.24 +0.4 3,808,563 - mutM b3635 0.24 -1.1 3,808,565 + mutM b3635 0.25 -0.5 3,808,614 - mutM b3635 0.31 -0.8 3,808,614 - mutM b3635 0.31 +0.3 3,808,699 - mutM b3635 0.41 -1.4 3,808,738 + mutM b3635 0.46 +0.3 3,808,753 - mutM b3635 0.48 +0.1 3,808,757 + mutM b3635 0.48 -0.5 3,808,758 - mutM b3635 0.48 +0.2 3,808,766 - mutM b3635 0.49 +1.6 3,808,784 + mutM b3635 0.52 +0.8 3,808,793 - mutM b3635 0.53 +0.3 3,808,796 - mutM b3635 0.53 +0.3 3,808,810 - mutM b3635 0.55 +0.2 3,808,824 - mutM b3635 0.57 +1.6 3,808,826 + mutM b3635 0.57 +0.7 3,808,833 + mutM b3635 0.58 +0.2 3,808,833 + mutM b3635 0.58 +1.2 3,808,834 - mutM b3635 0.58 +0.2 3,808,834 - mutM b3635 0.58 +0.0
Or see this region's nucleotide sequence