Strain Fitness in Escherichia coli BW25113 around b3073

Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaer and ygjG are separated by 417 nucleotidesygjG and ygjH are separated by 41 nucleotidesygjH and ebgR are separated by 218 nucleotides b3072: aer - fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component (NCBI), at 3,215,578 to 3,217,098 aer b3073: ygjG - putrescine:2-oxoglutaric acid aminotransferase, PLP-dependent (RefSeq), at 3,217,516 to 3,218,895 ygjG b3074: ygjH - hypothetical protein (NCBI), at 3,218,937 to 3,219,269 ygjH b3075: ebgR - DNA-binding transcriptional repressor (NCBI), at 3,219,488 to 3,220,471 ebgR Position (kb) 3217 3218 3219Strain fitness (log2 ratio) -2 -1 0 1 2at 3216.521 kb on - strand, within aerat 3216.521 kb on - strand, within aerat 3216.531 kb on - strand, within aerat 3216.597 kb on - strand, within aerat 3216.707 kb on + strand, within aerat 3216.759 kb on + strand, within aerat 3216.759 kb on + strand, within aerat 3216.808 kb on + strand, within aerat 3216.808 kb on + strand, within aerat 3216.958 kb on + strandat 3217.041 kb on - strandat 3217.041 kb on - strandat 3217.049 kb on - strandat 3217.086 kb on + strandat 3217.091 kb on - strandat 3217.091 kb on - strandat 3217.473 kb on - strandat 3217.638 kb on - strandat 3217.708 kb on + strand, within ygjGat 3217.708 kb on + strand, within ygjGat 3217.858 kb on + strand, within ygjGat 3217.858 kb on + strand, within ygjGat 3218.060 kb on + strand, within ygjGat 3218.112 kb on + strand, within ygjGat 3218.112 kb on + strand, within ygjGat 3218.118 kb on - strand, within ygjGat 3218.222 kb on + strand, within ygjGat 3218.231 kb on - strand, within ygjGat 3218.276 kb on - strand, within ygjGat 3218.293 kb on - strand, within ygjGat 3218.293 kb on - strand, within ygjGat 3218.338 kb on - strand, within ygjGat 3218.356 kb on + strand, within ygjGat 3218.387 kb on - strand, within ygjGat 3218.407 kb on - strand, within ygjGat 3218.407 kb on - strand, within ygjGat 3218.494 kb on + strand, within ygjGat 3218.532 kb on - strand, within ygjGat 3218.591 kb on - strand, within ygjGat 3218.591 kb on - strand, within ygjGat 3218.663 kb on - strand, within ygjGat 3218.663 kb on - strand, within ygjGat 3218.826 kb on + strandat 3218.833 kb on - strandat 3218.855 kb on + strandat 3218.959 kb on + strandat 3218.992 kb on + strand, within ygjHat 3218.992 kb on + strand, within ygjHat 3219.018 kb on + strand, within ygjHat 3219.018 kb on + strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.077 kb on - strand, within ygjHat 3219.117 kb on - strand, within ygjHat 3219.119 kb on + strand, within ygjHat 3219.384 kb on - strandat 3219.384 kb on - strandat 3219.431 kb on - strandat 3219.462 kb on + strandat 3219.549 kb on - strandat 3219.549 kb on - strandat 3219.628 kb on - strand, within ebgRat 3219.628 kb on - strand, within ebgRat 3219.650 kb on - strand, within ebgRat 3219.689 kb on + strand, within ebgRat 3219.691 kb on + strand, within ebgRat 3219.787 kb on + strand, within ebgRat 3219.803 kb on - strand, within ebgRat 3219.818 kb on - strand, within ebgRat 3219.861 kb on + strand, within ebgR

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM
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3,216,521 - aer b3072 0.62 -0.5
3,216,521 - aer b3072 0.62 +2.1
3,216,531 - aer b3072 0.63 -0.4
3,216,597 - aer b3072 0.67 +0.2
3,216,707 + aer b3072 0.74 +0.2
3,216,759 + aer b3072 0.78 -0.1
3,216,759 + aer b3072 0.78 -0.9
3,216,808 + aer b3072 0.81 +1.7
3,216,808 + aer b3072 0.81 -1.8
3,216,958 + -0.1
3,217,041 - +1.3
3,217,041 - -0.6
3,217,049 - +0.5
3,217,086 + -0.2
3,217,091 - +0.6
3,217,091 - +0.2
3,217,473 - -0.9
3,217,638 - -0.8
3,217,708 + ygjG b3073 0.14 +1.1
3,217,708 + ygjG b3073 0.14 -0.2
3,217,858 + ygjG b3073 0.25 +0.6
3,217,858 + ygjG b3073 0.25 -1.5
3,218,060 + ygjG b3073 0.39 +0.4
3,218,112 + ygjG b3073 0.43 +1.0
3,218,112 + ygjG b3073 0.43 +0.4
3,218,118 - ygjG b3073 0.44 +0.6
3,218,222 + ygjG b3073 0.51 +0.6
3,218,231 - ygjG b3073 0.52 -0.7
3,218,276 - ygjG b3073 0.55 +0.0
3,218,293 - ygjG b3073 0.56 -0.4
3,218,293 - ygjG b3073 0.56 +1.0
3,218,338 - ygjG b3073 0.60 +0.7
3,218,356 + ygjG b3073 0.61 -1.2
3,218,387 - ygjG b3073 0.63 +0.1
3,218,407 - ygjG b3073 0.65 -0.1
3,218,407 - ygjG b3073 0.65 -1.0
3,218,494 + ygjG b3073 0.71 -2.3
3,218,532 - ygjG b3073 0.74 +0.6
3,218,591 - ygjG b3073 0.78 -0.6
3,218,591 - ygjG b3073 0.78 -1.3
3,218,663 - ygjG b3073 0.83 +1.1
3,218,663 - ygjG b3073 0.83 -1.9
3,218,826 + +0.1
3,218,833 - +0.5
3,218,855 + +0.8
3,218,959 + -0.1
3,218,992 + ygjH b3074 0.17 +0.5
3,218,992 + ygjH b3074 0.17 -1.2
3,219,018 + ygjH b3074 0.24 -1.5
3,219,018 + ygjH b3074 0.24 +0.1
3,219,077 - ygjH b3074 0.42 +0.0
3,219,077 - ygjH b3074 0.42 -0.2
3,219,077 - ygjH b3074 0.42 -0.1
3,219,117 - ygjH b3074 0.54 -0.3
3,219,119 + ygjH b3074 0.55 +0.6
3,219,384 - +1.8
3,219,384 - +2.0
3,219,431 - +0.5
3,219,462 + -1.9
3,219,549 - -0.8
3,219,549 - -0.8
3,219,628 - ebgR b3075 0.14 +0.2
3,219,628 - ebgR b3075 0.14 +0.1
3,219,650 - ebgR b3075 0.16 +1.5
3,219,689 + ebgR b3075 0.20 +2.4
3,219,691 + ebgR b3075 0.21 +0.5
3,219,787 + ebgR b3075 0.30 -0.1
3,219,803 - ebgR b3075 0.32 +0.0
3,219,818 - ebgR b3075 0.34 +0.8
3,219,861 + ebgR b3075 0.38 +0.1

Or see this region's nucleotide sequence