Strain Fitness in Escherichia coli BW25113 around b2891

Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntlysS and prfB are separated by 9 nucleotidesprfB and recJ are separated by 90 nucleotides b2890: lysS - lysine tRNA synthetase, constitutive (NCBI), at 3,031,679 to 3,033,196 lysS b2891: prfB - peptide chain release factor RF-2 (NCBI), at 3,033,206 to 3,034,304 prfB b2892: recJ - ssDNA exonuclease, 5' --> 3'-specific (NCBI), at 3,034,395 to 3,036,128 recJ Position (kb) 3033 3034 3035Strain fitness (log2 ratio) -3 -2 -1 0 1at 3034.603 kb on - strand, within recJat 3034.661 kb on + strand, within recJat 3034.722 kb on + strand, within recJat 3034.911 kb on - strand, within recJat 3034.946 kb on - strand, within recJat 3035.015 kb on - strand, within recJat 3035.046 kb on + strand, within recJat 3035.110 kb on - strand, within recJat 3035.135 kb on - strand, within recJat 3035.270 kb on + strand, within recJ

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM
remove
3,034,603 - recJ b2892 0.12 -2.1
3,034,661 + recJ b2892 0.15 -2.3
3,034,722 + recJ b2892 0.19 -2.4
3,034,911 - recJ b2892 0.30 -1.8
3,034,946 - recJ b2892 0.32 -2.2
3,035,015 - recJ b2892 0.36 -2.5
3,035,046 + recJ b2892 0.38 -3.4
3,035,110 - recJ b2892 0.41 -1.8
3,035,135 - recJ b2892 0.43 -0.9
3,035,270 + recJ b2892 0.50 -2.5

Or see this region's nucleotide sequence