Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt srlR and gutQ overlap by 8 nucleotides gutQ and ygaA overlap by 4 nucleotides ygaA and norV are separated by 186 nucleotides
b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842
srlR
b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800
gutQ
b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311
ygaA
b2710: norV - anaerobic nitric oxide reductase flavorubredoxin (NCBI), at 2,830,498 to 2,831,937
norV
Position (kb)
2828
2829
2830
2831 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2827.893 kb on - strand at 2828.144 kb on + strand, within gutQ at 2828.242 kb on - strand, within gutQ at 2828.313 kb on - strand, within gutQ at 2828.314 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.405 kb on - strand, within gutQ at 2829.156 kb on - strand, within ygaA at 2829.156 kb on - strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.400 kb on + strand, within ygaA at 2829.417 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.544 kb on - strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA at 2829.846 kb on - strand, within ygaA at 2829.869 kb on - strand, within ygaA at 2829.871 kb on + strand, within ygaA at 2829.966 kb on + strand, within ygaA at 2829.979 kb on - strand, within ygaA at 2830.059 kb on - strand, within ygaA at 2830.059 kb on - strand, within ygaA at 2830.129 kb on - strand, within ygaA at 2830.141 kb on + strand, within ygaA at 2830.141 kb on + strand, within ygaA at 2830.152 kb on - strand, within ygaA at 2830.260 kb on - strand at 2830.683 kb on - strand, within norV at 2830.683 kb on - strand, within norV at 2830.708 kb on - strand, within norV at 2830.708 kb on - strand, within norV at 2830.743 kb on + strand, within norV at 2830.756 kb on - strand, within norV at 2830.756 kb on - strand, within norV at 2830.821 kb on + strand, within norV at 2830.983 kb on + strand, within norV at 2830.986 kb on - strand, within norV at 2830.986 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.011 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.018 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.075 kb on - strand, within norV at 2831.092 kb on - strand, within norV at 2831.109 kb on + strand, within norV at 2831.134 kb on + strand, within norV at 2831.273 kb on + strand, within norV at 2831.273 kb on + strand, within norV
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM remove 2,827,893 - +3.0 2,828,144 + gutQ b2708 0.32 +1.5 2,828,242 - gutQ b2708 0.42 +3.2 2,828,313 - gutQ b2708 0.49 +2.5 2,828,314 - gutQ b2708 0.50 +2.5 2,828,394 - gutQ b2708 0.58 +2.3 2,828,394 - gutQ b2708 0.58 +3.1 2,828,394 - gutQ b2708 0.58 +4.2 2,828,405 - gutQ b2708 0.59 +2.4 2,829,156 - ygaA b2709 0.24 -3.2 2,829,156 - ygaA b2709 0.24 -0.7 2,829,382 + ygaA b2709 0.39 -2.3 2,829,382 + ygaA b2709 0.39 +0.3 2,829,400 + ygaA b2709 0.40 +0.7 2,829,417 + ygaA b2709 0.41 -0.2 2,829,527 + ygaA b2709 0.48 +1.2 2,829,527 + ygaA b2709 0.48 -1.9 2,829,544 - ygaA b2709 0.49 -2.3 2,829,583 + ygaA b2709 0.52 +0.5 2,829,583 + ygaA b2709 0.52 -0.6 2,829,687 + ygaA b2709 0.59 +0.4 2,829,687 + ygaA b2709 0.59 -0.7 2,829,846 - ygaA b2709 0.69 -2.4 2,829,869 - ygaA b2709 0.71 -3.1 2,829,871 + ygaA b2709 0.71 +1.4 2,829,966 + ygaA b2709 0.77 +0.6 2,829,979 - ygaA b2709 0.78 +0.0 2,830,059 - ygaA b2709 0.83 +0.4 2,830,059 - ygaA b2709 0.83 +1.6 2,830,129 - ygaA b2709 0.88 +2.4 2,830,141 + ygaA b2709 0.89 +0.2 2,830,141 + ygaA b2709 0.89 +1.3 2,830,152 - ygaA b2709 0.89 +1.8 2,830,260 - -0.1 2,830,683 - norV b2710 0.13 +1.3 2,830,683 - norV b2710 0.13 +1.1 2,830,708 - norV b2710 0.15 -0.3 2,830,708 - norV b2710 0.15 +0.3 2,830,743 + norV b2710 0.17 -0.7 2,830,756 - norV b2710 0.18 -0.2 2,830,756 - norV b2710 0.18 -0.4 2,830,821 + norV b2710 0.22 -1.0 2,830,983 + norV b2710 0.34 -1.8 2,830,986 - norV b2710 0.34 -1.3 2,830,986 - norV b2710 0.34 +0.9 2,831,011 - norV b2710 0.36 +1.1 2,831,011 - norV b2710 0.36 +0.6 2,831,018 - norV b2710 0.36 +1.2 2,831,018 - norV b2710 0.36 -0.9 2,831,075 - norV b2710 0.40 +0.1 2,831,075 - norV b2710 0.40 +0.7 2,831,092 - norV b2710 0.41 -1.2 2,831,109 + norV b2710 0.42 +0.1 2,831,134 + norV b2710 0.44 +0.2 2,831,273 + norV b2710 0.54 -0.4 2,831,273 + norV b2710 0.54 +0.0
Or see this region's nucleotide sequence