Strain Fitness in Escherichia coli BW25113 around b2489

Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfG and hyfH are separated by 9 nucleotideshyfH and hyfI overlap by 4 nucleotideshyfI and b2490 overlap by 8 nucleotidesb2490 and hyfR are separated by 29 nucleotides b2487: hyfG - hydrogenase 4, subunit (NCBI), at 2,606,509 to 2,608,176 hyfG b2488: hyfH - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,186 to 2,608,731 hyfH b2489: hyfI - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,728 to 2,609,486 hyfI b2490: b2490 - putative protein processing element (VIMSS), at 2,609,479 to 2,609,892 b2490 b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934 hyfR Position (kb) 2608 2609 2610Strain fitness (log2 ratio) -2 -1 0 1 2at 2607.838 kb on - strand, within hyfGat 2607.879 kb on + strand, within hyfGat 2608.003 kb on - strand, within hyfGat 2608.030 kb on - strandat 2608.070 kb on - strandat 2608.089 kb on - strandat 2608.121 kb on + strandat 2608.121 kb on + strandat 2608.130 kb on - strandat 2608.223 kb on - strandat 2608.416 kb on - strand, within hyfHat 2608.416 kb on - strand, within hyfHat 2608.658 kb on - strand, within hyfHat 2608.658 kb on - strand, within hyfHat 2608.733 kb on - strandat 2608.733 kb on - strandat 2608.757 kb on - strandat 2609.013 kb on + strand, within hyfIat 2609.013 kb on + strand, within hyfIat 2609.095 kb on - strand, within hyfIat 2609.097 kb on + strand, within hyfIat 2609.290 kb on - strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.480 kb on + strandat 2609.482 kb on + strandat 2609.490 kb on - strandat 2609.490 kb on - strandat 2609.576 kb on - strand, within b2490at 2609.576 kb on - strand, within b2490at 2609.595 kb on - strand, within b2490at 2609.671 kb on - strand, within b2490at 2610.075 kb on - strandat 2610.080 kb on - strandat 2610.149 kb on - strand, within hyfRat 2610.261 kb on + strand, within hyfRat 2610.284 kb on - strand, within hyfRat 2610.286 kb on - strand, within hyfRat 2610.406 kb on + strand, within hyfRat 2610.411 kb on + strand, within hyfR

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM
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2,607,838 - hyfG b2487 0.80 +1.9
2,607,879 + hyfG b2487 0.82 -0.3
2,608,003 - hyfG b2487 0.90 +0.3
2,608,030 - +0.3
2,608,070 - -0.2
2,608,089 - -0.3
2,608,121 + +0.5
2,608,121 + +0.9
2,608,130 - -0.3
2,608,223 - -1.8
2,608,416 - hyfH b2488 0.42 -0.1
2,608,416 - hyfH b2488 0.42 -0.9
2,608,658 - hyfH b2488 0.86 +0.3
2,608,658 - hyfH b2488 0.86 -0.7
2,608,733 - +1.5
2,608,733 - -0.7
2,608,757 - -0.0
2,609,013 + hyfI b2489 0.38 +0.1
2,609,013 + hyfI b2489 0.38 +1.2
2,609,095 - hyfI b2489 0.48 +1.2
2,609,097 + hyfI b2489 0.49 +0.5
2,609,290 - hyfI b2489 0.74 +0.9
2,609,329 + hyfI b2489 0.79 +0.7
2,609,329 + hyfI b2489 0.79 -0.0
2,609,393 - hyfI b2489 0.88 +1.2
2,609,393 - hyfI b2489 0.88 +0.5
2,609,480 + -0.3
2,609,482 + +0.7
2,609,490 - -0.4
2,609,490 - +0.1
2,609,576 - b2490 b2490 0.23 -0.1
2,609,576 - b2490 b2490 0.23 -0.5
2,609,595 - b2490 b2490 0.28 -0.4
2,609,671 - b2490 b2490 0.46 +0.5
2,610,075 - -0.1
2,610,080 - +0.3
2,610,149 - hyfR b2491 0.11 -0.8
2,610,261 + hyfR b2491 0.17 +0.2
2,610,284 - hyfR b2491 0.18 -2.4
2,610,286 - hyfR b2491 0.18 -0.5
2,610,406 + hyfR b2491 0.24 +0.7
2,610,411 + hyfR b2491 0.24 -0.3

Or see this region's nucleotide sequence