Strain Fitness in Escherichia coli BW25113 around b1904

Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyecI and b1903 are separated by 78 nucleotidesb1903 and azuC are separated by 114 nucleotidesazuC and yecR are separated by 262 nucleotidesyecR and ftn are separated by 170 nucleotidesftn and yecH are separated by 37 nucleotides b1902: yecI - predicted ferritin-like protein (NCBI), at 1,984,949 to 1,985,452 yecI b4537: b1903 - orf, hypothetical protein (VIMSS), at 1,985,531 to 1,985,782 b1903 b4663: azuC - expressed protein (RefSeq), at 1,985,897 to 1,985,983 azuC b1904: yecR - hypothetical protein (NCBI), at 1,986,246 to 1,986,569 yecR b1905: ftn - ferritin iron storage protein (cytoplasmic) (NCBI), at 1,986,740 to 1,987,237 ftn b1906: yecH - hypothetical protein (NCBI), at 1,987,275 to 1,987,514 yecH Position (kb) 1986 1987Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 1985.263 kb on - strand, within yecIat 1985.263 kb on - strand, within yecIat 1985.330 kb on + strand, within yecIat 1985.369 kb on - strand, within yecIat 1985.369 kb on - strand, within yecIat 1985.410 kb on - strandat 1985.410 kb on - strandat 1985.428 kb on - strandat 1985.449 kb on + strandat 1985.449 kb on + strandat 1985.463 kb on + strandat 1985.821 kb on - strandat 1985.843 kb on + strandat 1985.843 kb on + strandat 1985.843 kb on + strandat 1985.935 kb on + strand, within azuCat 1986.136 kb on - strandat 1986.150 kb on - strandat 1986.175 kb on - strandat 1986.232 kb on - strandat 1986.479 kb on + strand, within yecRat 1986.479 kb on + strand, within yecRat 1986.479 kb on + strand, within yecRat 1986.521 kb on + strand, within yecRat 1986.533 kb on - strand, within yecRat 1986.533 kb on - strand, within yecRat 1986.715 kb on + strandat 1986.722 kb on + strandat 1986.732 kb on + strandat 1986.738 kb on - strandat 1986.740 kb on + strandat 1986.743 kb on - strandat 1986.743 kb on - strandat 1986.747 kb on + strandat 1986.755 kb on - strandat 1986.755 kb on - strandat 1986.773 kb on + strandat 1986.788 kb on + strandat 1986.791 kb on - strand, within ftnat 1986.791 kb on - strand, within ftnat 1986.791 kb on - strand, within ftnat 1986.798 kb on + strand, within ftnat 1986.801 kb on - strand, within ftnat 1986.801 kb on - strand, within ftnat 1986.829 kb on + strand, within ftnat 1986.829 kb on + strand, within ftnat 1986.832 kb on - strand, within ftnat 1986.837 kb on - strand, within ftnat 1986.844 kb on - strand, within ftnat 1986.850 kb on - strand, within ftnat 1986.850 kb on - strand, within ftnat 1986.863 kb on + strand, within ftnat 1986.863 kb on + strand, within ftnat 1986.863 kb on + strand, within ftnat 1986.863 kb on + strand, within ftnat 1986.863 kb on + strand, within ftnat 1986.868 kb on + strand, within ftnat 1986.873 kb on + strand, within ftnat 1986.885 kb on - strand, within ftnat 1986.896 kb on + strand, within ftnat 1986.896 kb on + strand, within ftnat 1986.896 kb on + strand, within ftnat 1986.908 kb on - strand, within ftnat 1986.922 kb on + strand, within ftnat 1986.997 kb on + strand, within ftnat 1986.997 kb on + strand, within ftnat 1987.022 kb on + strand, within ftnat 1987.056 kb on + strand, within ftnat 1987.056 kb on + strand, within ftnat 1987.059 kb on - strand, within ftnat 1987.059 kb on - strand, within ftnat 1987.064 kb on - strand, within ftnat 1987.064 kb on - strand, within ftnat 1987.064 kb on - strand, within ftnat 1987.064 kb on - strand, within ftnat 1987.070 kb on + strand, within ftnat 1987.078 kb on - strand, within ftnat 1987.080 kb on + strand, within ftnat 1987.105 kb on + strand, within ftnat 1987.105 kb on + strand, within ftnat 1987.110 kb on + strand, within ftnat 1987.110 kb on + strand, within ftnat 1987.112 kb on + strand, within ftnat 1987.112 kb on + strand, within ftnat 1987.113 kb on - strand, within ftnat 1987.118 kb on - strand, within ftnat 1987.123 kb on - strand, within ftnat 1987.123 kb on - strand, within ftnat 1987.127 kb on + strand, within ftnat 1987.132 kb on - strand, within ftnat 1987.157 kb on + strand, within ftnat 1987.211 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM
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1,985,263 - yecI b1902 0.62 -1.4
1,985,263 - yecI b1902 0.62 -1.0
1,985,330 + yecI b1902 0.76 +0.3
1,985,369 - yecI b1902 0.83 +3.8
1,985,369 - yecI b1902 0.83 -0.1
1,985,410 - -1.4
1,985,410 - +1.3
1,985,428 - -0.2
1,985,449 + -2.4
1,985,449 + +1.3
1,985,463 + +1.3
1,985,821 - -0.7
1,985,843 + +0.2
1,985,843 + -0.8
1,985,843 + +0.8
1,985,935 + azuC b4663 0.44 +1.3
1,986,136 - -1.2
1,986,150 - +0.2
1,986,175 - +0.4
1,986,232 - -0.1
1,986,479 + yecR b1904 0.72 -0.3
1,986,479 + yecR b1904 0.72 +1.6
1,986,479 + yecR b1904 0.72 -1.0
1,986,521 + yecR b1904 0.85 +1.1
1,986,533 - yecR b1904 0.89 +0.6
1,986,533 - yecR b1904 0.89 +0.6
1,986,715 + -0.4
1,986,722 + -1.0
1,986,732 + +0.6
1,986,738 - +1.6
1,986,740 + +0.6
1,986,743 - -0.4
1,986,743 - -1.1
1,986,747 + -0.8
1,986,755 - -1.0
1,986,755 - -1.0
1,986,773 + +0.6
1,986,788 + -0.4
1,986,791 - ftn b1905 0.10 -0.4
1,986,791 - ftn b1905 0.10 -1.0
1,986,791 - ftn b1905 0.10 +0.6
1,986,798 + ftn b1905 0.12 -0.4
1,986,801 - ftn b1905 0.12 +0.6
1,986,801 - ftn b1905 0.12 -0.4
1,986,829 + ftn b1905 0.18 -0.4
1,986,829 + ftn b1905 0.18 -1.0
1,986,832 - ftn b1905 0.18 +0.6
1,986,837 - ftn b1905 0.19 +0.6
1,986,844 - ftn b1905 0.21 +0.6
1,986,850 - ftn b1905 0.22 +0.6
1,986,850 - ftn b1905 0.22 +0.6
1,986,863 + ftn b1905 0.25 -2.6
1,986,863 + ftn b1905 0.25 +1.4
1,986,863 + ftn b1905 0.25 -1.4
1,986,863 + ftn b1905 0.25 -1.4
1,986,863 + ftn b1905 0.25 +0.6
1,986,868 + ftn b1905 0.26 -1.3
1,986,873 + ftn b1905 0.27 -0.4
1,986,885 - ftn b1905 0.29 +0.6
1,986,896 + ftn b1905 0.31 -1.4
1,986,896 + ftn b1905 0.31 +0.6
1,986,896 + ftn b1905 0.31 -0.4
1,986,908 - ftn b1905 0.34 +0.6
1,986,922 + ftn b1905 0.37 -0.3
1,986,997 + ftn b1905 0.52 +0.1
1,986,997 + ftn b1905 0.52 -0.6
1,987,022 + ftn b1905 0.57 +0.6
1,987,056 + ftn b1905 0.63 -0.2
1,987,056 + ftn b1905 0.63 -0.8
1,987,059 - ftn b1905 0.64 -1.0
1,987,059 - ftn b1905 0.64 -1.0
1,987,064 - ftn b1905 0.65 -1.8
1,987,064 - ftn b1905 0.65 +1.4
1,987,064 - ftn b1905 0.65 -1.0
1,987,064 - ftn b1905 0.65 +3.7
1,987,070 + ftn b1905 0.66 -2.4
1,987,078 - ftn b1905 0.68 +0.6
1,987,080 + ftn b1905 0.68 -2.9
1,987,105 + ftn b1905 0.73 -2.1
1,987,105 + ftn b1905 0.73 -0.7
1,987,110 + ftn b1905 0.74 +0.6
1,987,110 + ftn b1905 0.74 +0.2
1,987,112 + ftn b1905 0.75 +0.2
1,987,112 + ftn b1905 0.75 -1.9
1,987,113 - ftn b1905 0.75 -1.8
1,987,118 - ftn b1905 0.76 -0.9
1,987,123 - ftn b1905 0.77 -0.4
1,987,123 - ftn b1905 0.77 -1.4
1,987,127 + ftn b1905 0.78 +0.2
1,987,132 - ftn b1905 0.79 -0.2
1,987,157 + ftn b1905 0.84 -1.2
1,987,211 + -1.3

Or see this region's nucleotide sequence