Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioB and bioF overlap by 4 nucleotides bioF and bioC overlap by 14 nucleotides bioC and bioD overlap by 8 nucleotides
b0775: bioB - biotin synthase (NCBI), at 808,567 to 809,607
bioB
b0776: bioF - 8-amino-7-oxononanoate synthase (NCBI), at 809,604 to 810,758
bioF
b0777: bioC - predicted methltransferase, enzyme of biotin synthesis (NCBI), at 810,745 to 811,500
bioC
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
Position (kb) 809
810
811 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 808.684 kb on + strand, within bioB at 808.904 kb on - strand, within bioB at 808.915 kb on + strand, within bioB at 808.949 kb on + strand, within bioB at 808.964 kb on + strand, within bioB at 809.074 kb on + strand, within bioB at 809.082 kb on - strand, within bioB at 809.217 kb on - strand, within bioB at 809.217 kb on - strand, within bioB at 809.233 kb on + strand, within bioB at 809.379 kb on + strand, within bioB at 809.723 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 809.993 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.237 kb on + strand, within bioF at 810.359 kb on + strand, within bioF at 810.362 kb on - strand, within bioF at 810.393 kb on - strand, within bioF at 810.414 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.492 kb on + strand, within bioF at 810.673 kb on + strand at 810.673 kb on - strand at 810.729 kb on + strand at 810.791 kb on + strand at 810.833 kb on + strand, within bioC at 810.971 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.122 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.326 kb on + strand, within bioC at 811.366 kb on - strand, within bioC at 811.423 kb on + strand, within bioC at 811.423 kb on + strand, within bioC at 811.431 kb on - strand at 811.446 kb on - strand at 811.446 kb on - strand at 811.464 kb on - strand at 811.559 kb on + strand at 811.672 kb on + strand, within bioD at 811.720 kb on - strand, within bioD at 811.757 kb on + strand, within bioD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM remove 808,684 + bioB b0775 0.11 +1.6 808,904 - bioB b0775 0.32 -1.2 808,915 + bioB b0775 0.33 -0.6 808,949 + bioB b0775 0.37 +1.5 808,964 + bioB b0775 0.38 +0.4 809,074 + bioB b0775 0.49 +1.2 809,082 - bioB b0775 0.49 -0.1 809,217 - bioB b0775 0.62 -0.9 809,217 - bioB b0775 0.62 +0.1 809,233 + bioB b0775 0.64 +0.3 809,379 + bioB b0775 0.78 +1.8 809,723 + bioF b0776 0.10 +1.8 809,993 + bioF b0776 0.34 -0.6 809,993 + bioF b0776 0.34 -0.2 810,237 + bioF b0776 0.55 +1.2 810,237 + bioF b0776 0.55 +0.1 810,359 + bioF b0776 0.65 +0.5 810,362 - bioF b0776 0.66 -0.0 810,393 - bioF b0776 0.68 +0.6 810,414 + bioF b0776 0.70 -3.5 810,492 + bioF b0776 0.77 +1.4 810,492 + bioF b0776 0.77 -2.4 810,673 + -0.5 810,673 - -0.7 810,729 + +0.9 810,791 + +0.6 810,833 + bioC b0777 0.12 +2.5 810,971 + bioC b0777 0.30 -0.5 811,122 + bioC b0777 0.50 -1.6 811,122 + bioC b0777 0.50 -0.4 811,122 + bioC b0777 0.50 -1.4 811,122 + bioC b0777 0.50 -1.1 811,326 + bioC b0777 0.77 -0.8 811,326 + bioC b0777 0.77 +0.5 811,326 + bioC b0777 0.77 -2.7 811,366 - bioC b0777 0.82 -0.2 811,423 + bioC b0777 0.90 +0.6 811,423 + bioC b0777 0.90 -0.0 811,431 - -0.1 811,446 - -0.2 811,446 - +0.5 811,464 - +0.1 811,559 + +2.2 811,672 + bioD b0778 0.26 -0.4 811,720 - bioD b0778 0.33 -1.5 811,757 + bioD b0778 0.39 -1.2
Or see this region's nucleotide sequence