Experiment: LB Aerobic with Nitrofurantoin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fsr and ushA are separated by 217 nucleotides ushA and ybaK are separated by 36 nucleotides ybaK and sroB are separated by 121 nucleotides sroB and ybaP overlap by 2 nucleotides
b0479: fsr - predicted fosmidomycin efflux system (NCBI), at 502,700 to 503,920
fsr
b0480: ushA - UDP-sugar hydrolase (NCBI), at 504,138 to 505,790
ushA
b0481: ybaK - hypothetical protein (NCBI), at 505,827 to 506,306
ybaK
b4585: sroB - Novel sRNA, function unknown (RefSeq), at 506,428 to 506,511
sroB
b0482: ybaP - hypothetical protein (NCBI), at 506,510 to 507,304
ybaP
Position (kb) 504
505
506 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 503.178 kb on - strand, within fsr at 503.275 kb on - strand, within fsr at 503.288 kb on - strand, within fsr at 503.288 kb on - strand, within fsr at 503.555 kb on - strand, within fsr at 503.567 kb on + strand, within fsr at 503.575 kb on - strand, within fsr at 503.604 kb on - strand, within fsr at 503.616 kb on - strand, within fsr at 503.730 kb on - strand, within fsr at 503.734 kb on - strand, within fsr at 503.734 kb on - strand, within fsr at 503.924 kb on - strand at 503.985 kb on + strand at 503.985 kb on + strand at 504.067 kb on + strand at 504.181 kb on + strand at 504.345 kb on + strand, within ushA at 504.510 kb on + strand, within ushA at 504.510 kb on + strand, within ushA at 504.582 kb on - strand, within ushA at 504.582 kb on - strand, within ushA at 504.597 kb on - strand, within ushA at 504.750 kb on - strand, within ushA at 504.820 kb on + strand, within ushA at 504.959 kb on + strand, within ushA at 504.993 kb on + strand, within ushA at 505.007 kb on - strand, within ushA at 505.010 kb on + strand, within ushA at 505.105 kb on - strand, within ushA at 505.172 kb on + strand, within ushA at 505.172 kb on + strand, within ushA at 505.189 kb on - strand, within ushA at 505.189 kb on - strand, within ushA at 505.199 kb on - strand, within ushA at 505.199 kb on - strand, within ushA at 505.243 kb on - strand, within ushA at 505.283 kb on + strand, within ushA at 505.309 kb on - strand, within ushA at 505.354 kb on - strand, within ushA at 505.446 kb on + strand, within ushA at 505.506 kb on - strand, within ushA at 505.540 kb on - strand, within ushA at 505.540 kb on - strand, within ushA at 505.556 kb on - strand, within ushA at 505.636 kb on - strand at 505.645 kb on + strand at 505.671 kb on + strand at 505.719 kb on - strand at 505.725 kb on - strand at 506.002 kb on - strand, within ybaK at 506.006 kb on - strand, within ybaK at 506.020 kb on + strand, within ybaK at 506.057 kb on - strand, within ybaK at 506.057 kb on - strand, within ybaK at 506.122 kb on - strand, within ybaK at 506.122 kb on - strand, within ybaK at 506.288 kb on + strand at 506.343 kb on + strand at 506.350 kb on - strand at 506.424 kb on + strand at 506.443 kb on - strand, within sroB at 506.556 kb on - strand at 506.559 kb on - strand at 506.563 kb on - strand at 506.563 kb on - strand at 506.580 kb on - strand at 506.630 kb on + strand, within ybaP at 506.630 kb on + strand, within ybaP at 506.630 kb on + strand, within ybaP at 506.679 kb on - strand, within ybaP at 506.692 kb on + strand, within ybaP at 506.692 kb on + strand, within ybaP at 506.748 kb on - strand, within ybaP at 506.752 kb on - strand, within ybaP at 506.752 kb on - strand, within ybaP at 506.766 kb on - strand, within ybaP
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Nitrofurantoin 0.00003125 mM remove 503,178 - fsr b0479 0.39 +0.5 503,275 - fsr b0479 0.47 +0.2 503,288 - fsr b0479 0.48 -1.3 503,288 - fsr b0479 0.48 +0.9 503,555 - fsr b0479 0.70 +0.7 503,567 + fsr b0479 0.71 +1.5 503,575 - fsr b0479 0.72 -1.2 503,604 - fsr b0479 0.74 +0.6 503,616 - fsr b0479 0.75 +0.0 503,730 - fsr b0479 0.84 -1.0 503,734 - fsr b0479 0.85 +0.1 503,734 - fsr b0479 0.85 +0.4 503,924 - -0.6 503,985 + -1.1 503,985 + -0.6 504,067 + -2.3 504,181 + +0.0 504,345 + ushA b0480 0.13 +0.3 504,510 + ushA b0480 0.23 +1.2 504,510 + ushA b0480 0.23 +0.0 504,582 - ushA b0480 0.27 -1.5 504,582 - ushA b0480 0.27 -0.2 504,597 - ushA b0480 0.28 +1.2 504,750 - ushA b0480 0.37 +0.6 504,820 + ushA b0480 0.41 +0.9 504,959 + ushA b0480 0.50 +0.0 504,993 + ushA b0480 0.52 +1.1 505,007 - ushA b0480 0.53 +0.9 505,010 + ushA b0480 0.53 -0.1 505,105 - ushA b0480 0.58 +0.7 505,172 + ushA b0480 0.63 -1.0 505,172 + ushA b0480 0.63 -1.0 505,189 - ushA b0480 0.64 -0.6 505,189 - ushA b0480 0.64 -0.0 505,199 - ushA b0480 0.64 +1.5 505,199 - ushA b0480 0.64 +3.1 505,243 - ushA b0480 0.67 +0.3 505,283 + ushA b0480 0.69 +0.0 505,309 - ushA b0480 0.71 -1.6 505,354 - ushA b0480 0.74 +2.4 505,446 + ushA b0480 0.79 +0.7 505,506 - ushA b0480 0.83 +1.0 505,540 - ushA b0480 0.85 -0.8 505,540 - ushA b0480 0.85 +0.1 505,556 - ushA b0480 0.86 -0.6 505,636 - +1.2 505,645 + -1.0 505,671 + -0.4 505,719 - +1.0 505,725 - -0.3 506,002 - ybaK b0481 0.36 -2.3 506,006 - ybaK b0481 0.37 +0.3 506,020 + ybaK b0481 0.40 +0.3 506,057 - ybaK b0481 0.48 -0.8 506,057 - ybaK b0481 0.48 -0.8 506,122 - ybaK b0481 0.61 -0.4 506,122 - ybaK b0481 0.61 -0.5 506,288 + -0.1 506,343 + +0.1 506,350 - -0.8 506,424 + +0.3 506,443 - sroB b4585 0.18 -3.3 506,556 - -0.1 506,559 - +1.0 506,563 - +0.8 506,563 - +0.7 506,580 - +0.1 506,630 + ybaP b0482 0.15 -2.4 506,630 + ybaP b0482 0.15 +0.3 506,630 + ybaP b0482 0.15 +1.3 506,679 - ybaP b0482 0.21 +0.2 506,692 + ybaP b0482 0.23 -3.1 506,692 + ybaP b0482 0.23 -0.8 506,748 - ybaP b0482 0.30 -0.4 506,752 - ybaP b0482 0.30 +1.4 506,752 - ybaP b0482 0.30 -1.2 506,766 - ybaP b0482 0.32 +1.0
Or see this region's nucleotide sequence