Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ascB and hycI are separated by 158 nucleotides hycI and hycH overlap by 8 nucleotides hycH and hycG overlap by 4 nucleotides
b2716: ascB - cryptic 6-phospho-beta-glucosidase (NCBI), at 2,839,012 to 2,840,436
ascB
b2717: hycI - protease involved in processing C-terminal end of HycE (NCBI), at 2,840,595 to 2,841,065
hycI
b2718: hycH - protein required for maturation of hydrogenase 3 (NCBI), at 2,841,058 to 2,841,468
hycH
b2719: hycG - hydrogenase 3 and formate hydrogenase complex, HycG subunit (NCBI), at 2,841,465 to 2,842,232
hycG
Position (kb)
2840
2841
2842 Strain fitness (log2 ratio)
-1
0
1 at 2839.607 kb on + strand, within ascB at 2839.661 kb on - strand, within ascB at 2839.893 kb on - strand, within ascB at 2839.893 kb on - strand, within ascB at 2840.238 kb on + strand, within ascB at 2840.256 kb on - strand, within ascB at 2840.263 kb on + strand, within ascB at 2840.281 kb on - strand, within ascB at 2840.497 kb on - strand at 2840.551 kb on - strand at 2840.558 kb on + strand at 2840.644 kb on + strand, within hycI at 2840.688 kb on - strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.699 kb on + strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.711 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2840.731 kb on - strand, within hycI at 2841.008 kb on + strand, within hycI at 2841.071 kb on + strand at 2841.119 kb on + strand, within hycH at 2841.150 kb on + strand, within hycH at 2841.167 kb on + strand, within hycH at 2841.175 kb on - strand, within hycH at 2841.207 kb on + strand, within hycH at 2841.225 kb on + strand, within hycH at 2841.272 kb on - strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.300 kb on + strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.327 kb on - strand, within hycH at 2841.338 kb on - strand, within hycH at 2841.384 kb on - strand, within hycH at 2841.572 kb on + strand, within hycG at 2841.581 kb on - strand, within hycG at 2841.622 kb on - strand, within hycG at 2841.645 kb on - strand, within hycG at 2841.648 kb on - strand, within hycG at 2841.713 kb on + strand, within hycG at 2841.774 kb on - strand, within hycG at 2841.821 kb on - strand, within hycG at 2841.861 kb on - strand, within hycG at 2841.870 kb on - strand, within hycG at 2842.019 kb on + strand, within hycG at 2842.047 kb on - strand, within hycG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 2,839,607 + ascB b2716 0.42 -1.0 2,839,661 - ascB b2716 0.46 +0.5 2,839,893 - ascB b2716 0.62 +0.1 2,839,893 - ascB b2716 0.62 -0.3 2,840,238 + ascB b2716 0.86 -0.6 2,840,256 - ascB b2716 0.87 -0.1 2,840,263 + ascB b2716 0.88 +0.8 2,840,281 - ascB b2716 0.89 -0.3 2,840,497 - -0.2 2,840,551 - -0.8 2,840,558 + +1.1 2,840,644 + hycI b2717 0.10 +1.0 2,840,688 - hycI b2717 0.20 +1.0 2,840,699 + hycI b2717 0.22 +1.3 2,840,699 + hycI b2717 0.22 +1.1 2,840,711 - hycI b2717 0.25 +0.2 2,840,711 - hycI b2717 0.25 +0.4 2,840,731 - hycI b2717 0.29 -1.3 2,840,731 - hycI b2717 0.29 -0.8 2,841,008 + hycI b2717 0.88 +0.5 2,841,071 + +0.2 2,841,119 + hycH b2718 0.15 +0.7 2,841,150 + hycH b2718 0.22 +0.3 2,841,167 + hycH b2718 0.27 -0.4 2,841,175 - hycH b2718 0.28 +1.2 2,841,207 + hycH b2718 0.36 -0.1 2,841,225 + hycH b2718 0.41 -0.1 2,841,272 - hycH b2718 0.52 +0.3 2,841,300 + hycH b2718 0.59 +0.7 2,841,300 + hycH b2718 0.59 +0.3 2,841,327 - hycH b2718 0.65 +0.1 2,841,327 - hycH b2718 0.65 -0.0 2,841,338 - hycH b2718 0.68 +0.8 2,841,384 - hycH b2718 0.79 +0.6 2,841,572 + hycG b2719 0.14 -0.2 2,841,581 - hycG b2719 0.15 -0.1 2,841,622 - hycG b2719 0.20 -0.4 2,841,645 - hycG b2719 0.23 +1.0 2,841,648 - hycG b2719 0.24 +0.2 2,841,713 + hycG b2719 0.32 -0.5 2,841,774 - hycG b2719 0.40 +1.2 2,841,821 - hycG b2719 0.46 -0.2 2,841,861 - hycG b2719 0.52 -0.8 2,841,870 - hycG b2719 0.53 +1.0 2,842,019 + hycG b2719 0.72 +0.6 2,842,047 - hycG b2719 0.76 +0.2
Or see this region's nucleotide sequence