Experiment: LB Aerobic with Hygromycin B 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yojI and alkB are separated by 75 nucleotides alkB and ada overlap by 1 nucleotides ada and yojL are separated by 73 nucleotides
b2211: yojI - fused predicted multidrug transport subunits of ABC superfamily: membrane component/ATP-binding component (NCBI), at 2,304,994 to 2,306,637
yojI
b2212: alkB - oxidative demethylase of N1-methyladenine or N3-methylcytosine DNA lesions (NCBI), at 2,306,713 to 2,307,363
alkB
b2213: ada - fused DNA-binding transcriptional dual regulator/O6-methylguanine-DNA methyltransferase (NCBI), at 2,307,363 to 2,308,427
ada
b2214: yojL - predicted thiamine biosynthesis lipoprotein (NCBI), at 2,308,501 to 2,309,556
yojL
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2306.433 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.444 kb on - strand, within yojI at 2306.500 kb on + strand at 2306.572 kb on + strand at 2306.572 kb on + strand at 2306.783 kb on + strand, within alkB at 2306.801 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2306.942 kb on - strand, within alkB at 2307.059 kb on + strand, within alkB at 2307.168 kb on - strand, within alkB at 2307.181 kb on - strand, within alkB at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.336 kb on + strand at 2307.368 kb on - strand at 2307.393 kb on + strand at 2307.549 kb on - strand, within ada at 2307.562 kb on - strand, within ada at 2307.583 kb on + strand, within ada at 2307.593 kb on + strand, within ada at 2307.849 kb on - strand, within ada at 2308.153 kb on + strand, within ada at 2308.153 kb on + strand, within ada at 2308.162 kb on + strand, within ada at 2308.327 kb on + strand at 2308.337 kb on - strand at 2308.651 kb on + strand, within yojL at 2308.661 kb on - strand at 2308.661 kb on - strand, within yojL at 2308.745 kb on - strand, within yojL at 2308.784 kb on - strand, within yojL at 2308.796 kb on - strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.916 kb on + strand, within yojL at 2308.964 kb on - strand, within yojL at 2308.964 kb on - strand, within yojL at 2309.070 kb on - strand, within yojL at 2309.100 kb on + strand, within yojL at 2309.123 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL at 2309.286 kb on - strand, within yojL
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Hygromycin B 0.000015625 mM remove 2,306,433 - yojI b2211 0.88 -1.3 2,306,444 - yojI b2211 0.88 -0.6 2,306,444 - yojI b2211 0.88 -0.7 2,306,444 - yojI b2211 0.88 +0.7 2,306,500 + +0.7 2,306,572 + -1.3 2,306,572 + +0.3 2,306,783 + alkB b2212 0.11 -1.7 2,306,801 - alkB b2212 0.14 +1.3 2,306,942 - alkB b2212 0.35 +0.2 2,306,942 - alkB b2212 0.35 -0.2 2,307,059 + alkB b2212 0.53 -0.3 2,307,168 - alkB b2212 0.70 -0.2 2,307,181 - alkB b2212 0.72 +1.0 2,307,336 + -1.3 2,307,336 + +2.6 2,307,336 + -1.0 2,307,368 - -0.7 2,307,393 + -0.5 2,307,549 - ada b2213 0.17 +0.5 2,307,562 - ada b2213 0.19 -2.3 2,307,583 + ada b2213 0.21 -0.8 2,307,593 + ada b2213 0.22 -0.9 2,307,849 - ada b2213 0.46 -0.9 2,308,153 + ada b2213 0.74 -0.4 2,308,153 + ada b2213 0.74 -1.2 2,308,162 + ada b2213 0.75 -0.3 2,308,327 + -0.7 2,308,337 - -0.3 2,308,651 + yojL b2214 0.14 +0.7 2,308,661 - -1.0 2,308,661 - yojL b2214 0.15 +0.2 2,308,745 - yojL b2214 0.23 -1.9 2,308,784 - yojL b2214 0.27 +0.5 2,308,796 - yojL b2214 0.28 -0.6 2,308,916 + yojL b2214 0.39 -0.2 2,308,916 + yojL b2214 0.39 +0.4 2,308,964 - yojL b2214 0.44 -0.5 2,308,964 - yojL b2214 0.44 +0.5 2,309,070 - yojL b2214 0.54 +0.7 2,309,100 + yojL b2214 0.57 -0.4 2,309,123 - yojL b2214 0.59 +0.2 2,309,286 - yojL b2214 0.74 +0.7 2,309,286 - yojL b2214 0.74 +0.3
Or see this region's nucleotide sequence