Experiment: LB Aerobic with Tetracycline 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqhE and yqhG are separated by 199 nucleotides yqhG and yqhH are separated by 50 nucleotides yqhH and ygiQ are separated by 42 nucleotides
b3012: yqhE - orf, hypothetical protein (VIMSS), at 3,154,645 to 3,155,472
yqhE
b3013: yqhG - hypothetical protein (NCBI), at 3,155,672 to 3,156,598
yqhG
b3014: yqhH - predicted outer membrane lipoprotein (NCBI), at 3,156,649 to 3,156,906
yqhH
b4469: ygiQ - hypothetical protein (NCBI), at 3,156,949 to 3,159,168
ygiQ
Position (kb)
3155
3156
3157 Strain fitness (log2 ratio)
-1
0
1
2 at 3154.694 kb on + strand at 3154.702 kb on - strand at 3154.821 kb on - strand, within yqhE at 3154.821 kb on - strand, within yqhE at 3154.906 kb on + strand, within yqhE at 3154.909 kb on + strand, within yqhE at 3154.919 kb on + strand, within yqhE at 3154.963 kb on - strand, within yqhE at 3154.976 kb on - strand, within yqhE at 3154.976 kb on - strand, within yqhE at 3155.101 kb on + strand, within yqhE at 3155.121 kb on - strand, within yqhE at 3155.121 kb on - strand, within yqhE at 3155.160 kb on - strand, within yqhE at 3155.163 kb on + strand, within yqhE at 3155.163 kb on + strand, within yqhE at 3155.163 kb on + strand, within yqhE at 3155.271 kb on - strand, within yqhE at 3155.318 kb on + strand, within yqhE at 3155.318 kb on + strand, within yqhE at 3155.382 kb on - strand, within yqhE at 3155.382 kb on - strand, within yqhE at 3155.382 kb on - strand at 3155.382 kb on - strand, within yqhE at 3155.431 kb on + strand at 3155.449 kb on + strand at 3155.458 kb on - strand at 3155.493 kb on + strand at 3155.582 kb on + strand at 3155.601 kb on - strand at 3155.605 kb on + strand at 3155.627 kb on + strand at 3155.627 kb on + strand at 3155.630 kb on + strand at 3155.632 kb on + strand at 3155.632 kb on + strand at 3155.648 kb on + strand at 3155.726 kb on - strand at 3155.732 kb on + strand at 3155.734 kb on + strand at 3155.734 kb on + strand at 3155.738 kb on - strand at 3155.740 kb on - strand at 3155.740 kb on - strand at 3155.742 kb on - strand at 3155.750 kb on - strand at 3155.827 kb on + strand, within yqhG at 3155.827 kb on + strand, within yqhG at 3155.841 kb on + strand, within yqhG at 3155.844 kb on + strand, within yqhG at 3155.844 kb on + strand, within yqhG at 3155.846 kb on + strand, within yqhG at 3155.857 kb on + strand, within yqhG at 3155.861 kb on - strand, within yqhG at 3155.889 kb on + strand, within yqhG at 3155.955 kb on + strand, within yqhG at 3155.955 kb on + strand, within yqhG at 3155.981 kb on + strand, within yqhG at 3156.131 kb on - strand, within yqhG at 3156.363 kb on + strand, within yqhG at 3156.679 kb on - strand, within yqhH at 3156.691 kb on + strand, within yqhH at 3156.783 kb on + strand, within yqhH at 3156.783 kb on + strand, within yqhH at 3156.791 kb on - strand, within yqhH at 3156.791 kb on - strand, within yqhH at 3156.856 kb on + strand, within yqhH at 3156.859 kb on - strand, within yqhH at 3156.859 kb on - strand, within yqhH at 3157.016 kb on - strand at 3157.048 kb on - strand at 3157.087 kb on + strand at 3157.335 kb on + strand, within ygiQ at 3157.335 kb on + strand, within ygiQ at 3157.383 kb on - strand, within ygiQ at 3157.421 kb on + strand, within ygiQ at 3157.456 kb on - strand, within ygiQ at 3157.486 kb on - strand, within ygiQ at 3157.486 kb on - strand, within ygiQ at 3157.527 kb on + strand, within ygiQ at 3157.536 kb on + strand, within ygiQ at 3157.536 kb on + strand, within ygiQ at 3157.536 kb on + strand, within ygiQ at 3157.541 kb on + strand, within ygiQ at 3157.565 kb on + strand, within ygiQ at 3157.565 kb on + strand, within ygiQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Tetracycline 0.00000125 mM remove 3,154,694 + -0.1 3,154,702 - -1.0 3,154,821 - yqhE b3012 0.21 +0.1 3,154,821 - yqhE b3012 0.21 +0.7 3,154,906 + yqhE b3012 0.32 -0.6 3,154,909 + yqhE b3012 0.32 -0.4 3,154,919 + yqhE b3012 0.33 +0.1 3,154,963 - yqhE b3012 0.38 +1.7 3,154,976 - yqhE b3012 0.40 -1.4 3,154,976 - yqhE b3012 0.40 -0.7 3,155,101 + yqhE b3012 0.55 +0.8 3,155,121 - yqhE b3012 0.57 +1.3 3,155,121 - yqhE b3012 0.57 +1.3 3,155,160 - yqhE b3012 0.62 -0.6 3,155,163 + yqhE b3012 0.63 +0.8 3,155,163 + yqhE b3012 0.63 +1.1 3,155,163 + yqhE b3012 0.63 +0.2 3,155,271 - yqhE b3012 0.76 -0.2 3,155,318 + yqhE b3012 0.81 -0.1 3,155,318 + yqhE b3012 0.81 +0.7 3,155,382 - yqhE b3012 0.89 +0.2 3,155,382 - yqhE b3012 0.89 -0.3 3,155,382 - +0.3 3,155,382 - yqhE b3012 0.89 +1.5 3,155,431 + +1.2 3,155,449 + +0.5 3,155,458 - +1.3 3,155,493 + +2.3 3,155,582 + +0.7 3,155,601 - +0.8 3,155,605 + -1.3 3,155,627 + +1.1 3,155,627 + +0.8 3,155,630 + +0.8 3,155,632 + +1.3 3,155,632 + +0.3 3,155,648 + +1.6 3,155,726 - +0.0 3,155,732 + -0.1 3,155,734 + -0.6 3,155,734 + +0.6 3,155,738 - -0.0 3,155,740 - -0.4 3,155,740 - +0.7 3,155,742 - +0.7 3,155,750 - -1.2 3,155,827 + yqhG b3013 0.17 +0.3 3,155,827 + yqhG b3013 0.17 +0.6 3,155,841 + yqhG b3013 0.18 +0.2 3,155,844 + yqhG b3013 0.19 +0.3 3,155,844 + yqhG b3013 0.19 +0.1 3,155,846 + yqhG b3013 0.19 -1.5 3,155,857 + yqhG b3013 0.20 +0.3 3,155,861 - yqhG b3013 0.20 -0.3 3,155,889 + yqhG b3013 0.23 +0.5 3,155,955 + yqhG b3013 0.31 -1.7 3,155,955 + yqhG b3013 0.31 +0.2 3,155,981 + yqhG b3013 0.33 +0.5 3,156,131 - yqhG b3013 0.50 +1.7 3,156,363 + yqhG b3013 0.75 -0.1 3,156,679 - yqhH b3014 0.12 -0.2 3,156,691 + yqhH b3014 0.16 +0.2 3,156,783 + yqhH b3014 0.52 -1.2 3,156,783 + yqhH b3014 0.52 -0.2 3,156,791 - yqhH b3014 0.55 +0.4 3,156,791 - yqhH b3014 0.55 +0.8 3,156,856 + yqhH b3014 0.80 -0.3 3,156,859 - yqhH b3014 0.81 +0.1 3,156,859 - yqhH b3014 0.81 +0.5 3,157,016 - +0.2 3,157,048 - -0.8 3,157,087 + +0.8 3,157,335 + ygiQ b4469 0.17 -0.6 3,157,335 + ygiQ b4469 0.17 +0.3 3,157,383 - ygiQ b4469 0.20 +0.6 3,157,421 + ygiQ b4469 0.21 +0.7 3,157,456 - ygiQ b4469 0.23 +0.2 3,157,486 - ygiQ b4469 0.24 +0.4 3,157,486 - ygiQ b4469 0.24 -0.1 3,157,527 + ygiQ b4469 0.26 +0.7 3,157,536 + ygiQ b4469 0.26 +1.1 3,157,536 + ygiQ b4469 0.26 +0.0 3,157,536 + ygiQ b4469 0.26 -0.6 3,157,541 + ygiQ b4469 0.27 +0.3 3,157,565 + ygiQ b4469 0.28 -0.3 3,157,565 + ygiQ b4469 0.28 +0.6
Or see this region's nucleotide sequence