Strain Fitness in Escherichia coli BW25113 around b3533

Experiment: LB Aerobic with Trimethoprim 0.00000125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Aerobic with Trimethoprim 0.00000125 mM
3,689,666	-	bcsB	b3532	0.59	-0.2
3,689,682	-	bcsB	b3532	0.59	-0.0
3,689,731	-	bcsB	b3532	0.62	-0.6
3,689,837	-	bcsB	b3532	0.66	-1.1
3,689,837	-	bcsB	b3532	0.66	+1.1
3,689,862	-	bcsB	b3532	0.67	-1.1
3,689,889	-	bcsB	b3532	0.68	-1.8
3,689,942	+	bcsB	b3532	0.71	-0.6
3,689,964	+	bcsB	b3532	0.71	+0.2
3,689,964	+	bcsB	b3532	0.71	+0.4
3,689,979	-	bcsB	b3532	0.72	-0.8
3,689,979	-	bcsB	b3532	0.72	+0.8
3,689,988	+	bcsB	b3532	0.73	-1.6
3,689,988	+	bcsB	b3532	0.73	+2.3
3,690,002	+	bcsB	b3532	0.73	+0.4
3,690,002	+	bcsB	b3532	0.73	+0.5
3,690,012	+	bcsB	b3532	0.74	+0.2
3,690,012	+	bcsB	b3532	0.74	-0.4
3,690,017	+				-0.0
3,690,017	+	bcsB	b3532	0.74	-1.6
3,690,023	+	bcsB	b3532	0.74	+0.3
3,690,035	+	bcsB	b3532	0.75	+0.5
3,690,035	+	bcsB	b3532	0.75	-0.1
3,690,059	-	bcsB	b3532	0.76	-0.7
3,690,059	-	bcsB	b3532	0.76	+0.4
3,690,098	-	bcsB	b3532	0.77	-1.0
3,690,120	-	bcsB	b3532	0.78	-0.2
3,690,236	+	bcsB	b3532	0.83	-0.7
3,690,252	+	bcsB	b3532	0.84	-0.4
3,690,265	+	bcsB	b3532	0.84	+0.2
3,690,283	-	bcsB	b3532	0.85	-0.1
3,690,283	-	bcsB	b3532	0.85	-0.6
3,690,314	-	bcsB	b3532	0.86	-1.0
3,690,361	+	bcsB	b3532	0.88	-0.1
3,690,361	+	bcsB	b3532	0.88	+0.8
3,690,361	+	bcsB	b3532	0.88	+0.5
3,690,369	-	bcsB	b3532	0.89	+0.2
3,690,369	-	bcsB	b3532	0.89	-0.2
3,690,379	-	bcsB	b3532	0.89	+0.2
3,690,408	+				+0.1
3,690,414	+				+0.7
3,690,450	-				+0.3
3,690,460	+				-1.1
3,690,468	-				+0.2
3,690,468	-				+0.7
3,690,516	+				+0.4
3,690,535	-				-0.7
3,690,535	-				-0.4
3,690,535	-				+0.1
3,690,572	+				-0.1
3,690,572	+				-0.3
3,690,577	-				-0.3
3,690,577	-				+0.6
3,690,590	+				+0.1
3,690,649	-				+0.0
3,690,649	-				+0.3
3,690,659	-				-0.3
3,690,665	-				-0.2
3,690,669	-				-0.1
3,690,711	-				+0.0
3,690,718	-				+1.0
3,690,718	-				+0.3
3,690,742	-				+0.8
3,690,773	-				+0.1
3,690,786	-				-0.1
3,690,793	+				+0.4
3,690,887	+				+0.6
3,690,891	+				-0.2
3,690,891	+				+0.0
3,690,891	+				+0.7
3,690,891	+				-1.0
3,690,897	-				+0.3
3,690,899	+				-0.2
3,690,899	-				+0.2
3,690,899	-				+0.4
3,690,932	-	yhjO	b3533	0.11	-0.7
3,691,038	+	yhjO	b3533	0.15	-0.4
3,691,101	+	yhjO	b3533	0.18	-0.3
3,691,109	-	yhjO	b3533	0.18	-0.7
3,691,109	-	yhjO	b3533	0.18	-0.3
3,691,109	-	yhjO	b3533	0.18	-0.1
3,691,112	+	yhjO	b3533	0.18	-0.1
3,691,112	+	yhjO	b3533	0.18	-0.0
3,691,121	-	yhjO	b3533	0.18	-0.2
3,691,190	+	yhjO	b3533	0.21	-0.0
3,691,244	-	yhjO	b3533	0.23	-0.0
3,691,245	+	yhjO	b3533	0.23	-0.6
3,691,245	+	yhjO	b3533	0.23	+0.2
3,691,251	-	yhjO	b3533	0.23	-1.1
3,691,251	-	yhjO	b3533	0.23	-0.9
3,691,324	-	yhjO	b3533	0.26	+1.2
3,691,341	+	yhjO	b3533	0.27	-1.8
3,691,341	+	yhjO	b3533	0.27	-0.6
3,691,361	-	yhjO	b3533	0.27	-1.3
3,691,361	-	yhjO	b3533	0.27	-0.1
3,691,455	+	yhjO	b3533	0.31	+0.7
3,691,455	+	yhjO	b3533	0.31	-0.0
3,691,455	+	yhjO	b3533	0.31	-1.5
3,691,466	+	yhjO	b3533	0.32	-0.8
3,691,468	-	yhjO	b3533	0.32	+0.0
3,691,500	+	yhjO	b3533	0.33	-0.1
3,691,559	-	yhjO	b3533	0.35	-0.6
3,691,643	-	yhjO	b3533	0.38	-0.8
3,691,686	+	yhjO	b3533	0.40	-1.0
3,691,753	+	yhjO	b3533	0.42	-1.6
3,691,753	+	yhjO	b3533	0.42	-0.5
3,691,756	-	yhjO	b3533	0.43	+0.1
3,691,836	-	yhjO	b3533	0.46	-0.4
3,691,841	-	yhjO	b3533	0.46	-0.7
3,691,841	-	yhjO	b3533	0.46	-0.3
3,691,941	+	yhjO	b3533	0.50	+0.4
3,692,087	-	yhjO	b3533	0.55	-1.3
3,692,135	+	yhjO	b3533	0.57	+0.4
3,692,143	-	yhjO	b3533	0.57	-0.4
3,692,147	-	yhjO	b3533	0.58	+0.1
3,692,225	+	yhjO	b3533	0.60	-0.2
3,692,225	+	yhjO	b3533	0.60	+0.2
3,692,248	+	yhjO	b3533	0.61	-0.4
3,692,263	+	yhjO	b3533	0.62	+0.1
3,692,276	-	yhjO	b3533	0.62	+0.7
3,692,412	+	yhjO	b3533	0.68	+1.4
3,692,440	-	yhjO	b3533	0.69	-0.4
3,692,507	-	yhjO	b3533	0.71	-0.3
3,692,507	-	yhjO	b3533	0.71	+0.2
3,692,510	+	yhjO	b3533	0.71	-0.2
3,692,510	+	yhjO	b3533	0.71	-0.3
3,692,510	+	yhjO	b3533	0.71	-1.8
3,692,521	+	yhjO	b3533	0.72	-0.8
3,692,557	-	yhjO	b3533	0.73	-0.4
3,692,562	-	yhjO	b3533	0.73	-0.6
3,692,562	-	yhjO	b3533	0.73	+0.5
3,692,566	+	yhjO	b3533	0.74	-0.5
3,692,567	+	yhjO	b3533	0.74	-2.0
3,692,579	-	yhjO	b3533	0.74	-0.3
3,692,655	-	yhjO	b3533	0.77	-0.1
3,692,706	+	yhjO	b3533	0.79	-1.3
3,692,706	+	yhjO	b3533	0.79	+0.3
3,692,706	-	yhjO	b3533	0.79	+0.1
3,692,706	-	yhjO	b3533	0.79	+1.3
3,692,714	-	yhjO	b3533	0.79	-0.5
3,692,714	-	yhjO	b3533	0.79	-0.6
3,692,755	-	yhjO	b3533	0.81	-0.0
3,692,845	+	yhjO	b3533	0.84	+0.0
3,692,862	-	yhjO	b3533	0.85	-0.0
3,693,033	-				-0.2
3,693,102	-				-1.0
3,693,128	+				+0.6
3,693,128	+				-0.6
3,693,136	-				+0.8
3,693,177	-				-0.1
3,693,242	-				+0.2
3,693,262	-				-0.6
3,693,285	+				+0.0
3,693,285	+				-0.0
3,693,290	+				-0.1
3,693,293	-				-0.6
3,693,293	-				-0.4
3,693,328	-				+1.3
3,693,333	-	yhjQ	b3534	0.10	+0.4
3,693,333	-	yhjQ	b3534	0.10	-0.8
3,693,355	-	yhjQ	b3534	0.13	+0.2
3,693,375	-	yhjQ	b3534	0.16	-0.2
3,693,375	-	yhjQ	b3534	0.16	+0.2
3,693,386	+	yhjQ	b3534	0.17	-0.2
3,693,421	+	yhjQ	b3534	0.22	-0.9
3,693,464	-	yhjQ	b3534	0.28	+0.5
3,693,467	+	yhjQ	b3534	0.28	-0.6
3,693,481	+	yhjQ	b3534	0.30	-0.0
3,693,533	-	yhjQ	b3534	0.37	+2.0
3,693,533	-	yhjQ	b3534	0.37	-0.0
3,693,631	-	yhjQ	b3534	0.50	+0.2
3,693,713	+	yhjQ	b3534	0.61	-0.0
3,693,713	+	yhjQ	b3534	0.61	-0.2
3,693,751	-	yhjQ	b3534	0.66	+0.4
3,693,781	+	yhjQ	b3534	0.70	+0.2
3,693,782	+	yhjQ	b3534	0.70	+1.1
3,693,782	+	yhjQ	b3534	0.70	-1.2
3,693,790	-	yhjQ	b3534	0.71	-0.7
3,693,790	-	yhjQ	b3534	0.71	-0.3
3,693,800	-	yhjQ	b3534	0.72	+0.4
3,693,803	+	yhjQ	b3534	0.73	+0.4
3,693,808	+	yhjQ	b3534	0.73	-0.8
3,693,808	+	yhjQ	b3534	0.73	-0.4
3,693,816	-	yhjQ	b3534	0.74	-1.0
3,693,816	-	yhjQ	b3534	0.74	-0.2
3,693,827	+	yhjQ	b3534	0.76	+0.2
3,693,827	+	yhjQ	b3534	0.76	-0.3
3,693,828	+	yhjQ	b3534	0.76	-1.1
3,693,838	+	yhjQ	b3534	0.77	-0.6
3,693,838	+	yhjQ	b3534	0.77	-0.0
3,693,874	+	yhjQ	b3534	0.82	-0.4
3,693,882	-	yhjQ	b3534	0.83	+1.7
3,693,882	-	yhjQ	b3534	0.83	+1.1
3,693,899	-	yhjQ	b3534	0.85	+0.4
3,693,913	-	yhjQ	b3534	0.87	-0.3
3,693,948	-				+0.2
3,693,984	+				-1.4
3,693,984	+				-0.6
3,693,992	-				+1.2
3,693,992	-				-0.2
3,693,997	+				+0.2
3,693,997	+				-0.1
3,694,039	-	yhjR	b3535	0.10	+0.5
3,694,079	-	yhjR	b3535	0.31	-0.5
3,694,110	+	yhjR	b3535	0.48	-0.1
3,694,110	+	yhjR	b3535	0.48	+0.2
3,694,112	-	yhjR	b3535	0.49	+0.5
3,694,116	-	yhjR	b3535	0.51	-0.9
3,694,116	-	yhjR	b3535	0.51	+0.1
3,694,154	+	yhjR	b3535	0.71	+1.1
3,694,165	-	yhjR	b3535	0.77	-0.3
3,694,174	+	yhjR	b3535	0.81	-1.2
3,694,186	+	yhjR	b3535	0.88	-0.5
3,694,186	+	yhjR	b3535	0.88	-0.6
3,694,194	-				-0.1
3,694,215	-				-0.9