Experiment: LB Aerobic with Trimethoprim 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dacC and deoR are separated by 46 nucleotides deoR and ybjG are separated by 57 nucleotides ybjG and cmr are separated by 284 nucleotides
b0839: dacC - D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 6a) (NCBI), at 879,950 to 881,152
dacC
b0840: deoR - DNA-binding transcriptional repressor (NCBI), at 881,199 to 881,957
deoR
b0841: ybjG - undecaprenyl pyrophosphate phosphatase (NCBI), at 882,015 to 882,611
ybjG
b0842: cmr - multidrug efflux system protein (NCBI), at 882,896 to 884,128
cmr
Position (kb) 881
882 Strain fitness (log2 ratio) -2
-1
0
1
2 at 880.270 kb on - strand, within dacC at 880.298 kb on + strand, within dacC at 880.298 kb on + strand, within dacC at 880.306 kb on + strand, within dacC at 880.453 kb on + strand, within dacC at 880.472 kb on - strand, within dacC at 880.489 kb on + strand, within dacC at 880.524 kb on - strand, within dacC at 880.550 kb on + strand, within dacC at 880.550 kb on + strand, within dacC at 880.568 kb on + strand, within dacC at 880.676 kb on + strand, within dacC at 880.829 kb on + strand, within dacC at 880.829 kb on + strand, within dacC at 880.917 kb on + strand, within dacC at 880.917 kb on + strand, within dacC at 881.000 kb on + strand, within dacC at 881.094 kb on + strand at 881.094 kb on + strand at 881.146 kb on - strand at 881.159 kb on + strand at 881.228 kb on - strand at 881.283 kb on - strand, within deoR at 881.294 kb on - strand, within deoR at 881.371 kb on + strand, within deoR at 881.497 kb on + strand, within deoR at 881.588 kb on - strand, within deoR at 881.661 kb on - strand, within deoR at 881.765 kb on + strand, within deoR at 881.778 kb on - strand, within deoR at 881.778 kb on - strand, within deoR at 881.829 kb on + strand, within deoR at 881.878 kb on + strand, within deoR at 881.878 kb on + strand, within deoR at 881.902 kb on - strand at 881.906 kb on + strand at 881.906 kb on + strand at 881.957 kb on - strand at 881.957 kb on - strand at 882.047 kb on + strand at 882.231 kb on + strand, within ybjG at 882.251 kb on - strand, within ybjG at 882.298 kb on - strand, within ybjG at 882.418 kb on - strand, within ybjG at 882.455 kb on + strand, within ybjG at 882.485 kb on - strand, within ybjG at 882.485 kb on - strand, within ybjG at 882.551 kb on - strand, within ybjG at 882.589 kb on - strand at 882.645 kb on + strand at 882.664 kb on - strand at 882.671 kb on + strand at 882.695 kb on + strand at 882.719 kb on - strand at 882.723 kb on + strand at 882.731 kb on - strand at 882.738 kb on + strand at 882.745 kb on - strand at 882.746 kb on + strand at 882.746 kb on + strand at 882.785 kb on - strand at 882.802 kb on - strand at 882.804 kb on + strand at 882.819 kb on - strand at 882.819 kb on - strand at 882.819 kb on - strand at 882.918 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Trimethoprim 0.00000125 mM remove 880,270 - dacC b0839 0.27 -1.2 880,298 + dacC b0839 0.29 -0.5 880,298 + dacC b0839 0.29 -0.2 880,306 + dacC b0839 0.30 +0.9 880,453 + dacC b0839 0.42 +0.9 880,472 - dacC b0839 0.43 +0.3 880,489 + dacC b0839 0.45 -0.3 880,524 - dacC b0839 0.48 -1.1 880,550 + dacC b0839 0.50 -1.2 880,550 + dacC b0839 0.50 -0.3 880,568 + dacC b0839 0.51 +0.3 880,676 + dacC b0839 0.60 +0.1 880,829 + dacC b0839 0.73 +0.2 880,829 + dacC b0839 0.73 +0.5 880,917 + dacC b0839 0.80 +0.3 880,917 + dacC b0839 0.80 +0.3 881,000 + dacC b0839 0.87 -1.0 881,094 + -0.4 881,094 + +0.9 881,146 - -0.7 881,159 + +0.3 881,228 - -1.5 881,283 - deoR b0840 0.11 +0.8 881,294 - deoR b0840 0.13 -0.8 881,371 + deoR b0840 0.23 -0.4 881,497 + deoR b0840 0.39 -1.1 881,588 - deoR b0840 0.51 -1.9 881,661 - deoR b0840 0.61 -0.3 881,765 + deoR b0840 0.75 +1.9 881,778 - deoR b0840 0.76 -0.8 881,778 - deoR b0840 0.76 -1.2 881,829 + deoR b0840 0.83 -0.7 881,878 + deoR b0840 0.89 -0.7 881,878 + deoR b0840 0.89 -1.2 881,902 - -0.9 881,906 + -1.2 881,906 + +0.3 881,957 - -1.7 881,957 - -0.5 882,047 + +0.7 882,231 + ybjG b0841 0.36 +0.9 882,251 - ybjG b0841 0.40 +0.5 882,298 - ybjG b0841 0.47 +0.9 882,418 - ybjG b0841 0.68 -0.7 882,455 + ybjG b0841 0.74 +0.3 882,485 - ybjG b0841 0.79 -0.8 882,485 - ybjG b0841 0.79 +0.7 882,551 - ybjG b0841 0.90 -0.2 882,589 - +1.3 882,645 + +0.2 882,664 - -2.0 882,671 + +0.1 882,695 + -1.8 882,719 - -0.1 882,723 + +0.3 882,731 - -0.0 882,738 + -0.4 882,745 - +0.1 882,746 + +0.1 882,746 + +1.5 882,785 - +0.3 882,802 - +0.5 882,804 + -0.1 882,819 - -0.1 882,819 - -1.0 882,819 - -0.7 882,918 + -1.2
Or see this region's nucleotide sequence