Experiment: LB Aerobic with Trimethoprim 0.00000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gcd and hpt are separated by 205 nucleotides hpt and can are separated by 40 nucleotides can and yadG are separated by 108 nucleotides
b0124: gcd - glucose dehydrogenase (NCBI), at 138,835 to 141,225
gcd
b0125: hpt - hypoxanthine phosphoribosyltransferase (VIMSS), at 141,431 to 141,967
hpt
b0126: can - carbonic anhydrase (NCBI), at 142,008 to 142,670
can
b0127: yadG - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 142,779 to 143,705
yadG
Position (kb) 141
142 Strain fitness (log2 ratio) -2
-1
0
1
2 at 140.466 kb on + strand, within gcd at 140.541 kb on + strand, within gcd at 140.542 kb on - strand, within gcd at 140.542 kb on - strand, within gcd at 140.576 kb on - strand, within gcd at 140.580 kb on - strand, within gcd at 140.630 kb on + strand, within gcd at 140.634 kb on - strand, within gcd at 140.663 kb on + strand, within gcd at 140.710 kb on + strand, within gcd at 140.710 kb on + strand, within gcd at 140.733 kb on - strand, within gcd at 140.733 kb on - strand, within gcd at 140.744 kb on - strand, within gcd at 140.744 kb on - strand, within gcd at 140.818 kb on + strand, within gcd at 140.818 kb on + strand, within gcd at 140.826 kb on - strand, within gcd at 140.826 kb on - strand, within gcd at 140.842 kb on - strand, within gcd at 140.854 kb on - strand, within gcd at 140.854 kb on - strand, within gcd at 140.856 kb on + strand, within gcd at 141.007 kb on + strand at 141.010 kb on + strand at 141.059 kb on + strand at 141.067 kb on - strand at 141.078 kb on + strand at 141.086 kb on - strand at 141.095 kb on + strand at 141.103 kb on - strand at 141.103 kb on - strand at 141.134 kb on + strand at 141.134 kb on + strand at 141.182 kb on - strand at 141.229 kb on - strand at 141.365 kb on + strand at 141.513 kb on - strand, within hpt at 141.566 kb on + strand, within hpt at 141.566 kb on + strand, within hpt at 141.574 kb on - strand, within hpt at 141.574 kb on - strand, within hpt at 141.586 kb on + strand, within hpt at 141.588 kb on + strand, within hpt at 141.588 kb on + strand, within hpt at 141.591 kb on - strand, within hpt at 141.591 kb on - strand, within hpt at 141.618 kb on + strand, within hpt at 141.899 kb on + strand, within hpt at 141.906 kb on + strand, within hpt at 141.910 kb on + strand, within hpt at 141.910 kb on + strand, within hpt at 141.967 kb on + strand at 142.000 kb on - strand at 142.000 kb on - strand at 142.836 kb on - strand at 142.836 kb on - strand at 142.925 kb on - strand, within yadG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Aerobic with Trimethoprim 0.00000125 mM remove 140,466 + gcd b0124 0.68 +0.0 140,541 + gcd b0124 0.71 +0.5 140,542 - gcd b0124 0.71 -0.3 140,542 - gcd b0124 0.71 +0.6 140,576 - gcd b0124 0.73 -1.0 140,580 - gcd b0124 0.73 +0.6 140,630 + gcd b0124 0.75 +0.8 140,634 - gcd b0124 0.75 -0.5 140,663 + gcd b0124 0.76 -0.1 140,710 + gcd b0124 0.78 +0.7 140,710 + gcd b0124 0.78 -0.3 140,733 - gcd b0124 0.79 -0.6 140,733 - gcd b0124 0.79 +0.2 140,744 - gcd b0124 0.80 -0.4 140,744 - gcd b0124 0.80 +0.8 140,818 + gcd b0124 0.83 -0.3 140,818 + gcd b0124 0.83 -0.6 140,826 - gcd b0124 0.83 -0.6 140,826 - gcd b0124 0.83 -0.1 140,842 - gcd b0124 0.84 +0.0 140,854 - gcd b0124 0.84 -2.0 140,854 - gcd b0124 0.84 +0.2 140,856 + gcd b0124 0.85 +0.8 141,007 + -0.2 141,010 + -0.4 141,059 + -0.2 141,067 - +0.2 141,078 + -1.7 141,086 - -0.2 141,095 + +0.4 141,103 - -0.4 141,103 - +2.6 141,134 + +1.4 141,134 + +1.2 141,182 - -0.4 141,229 - -0.9 141,365 + +0.5 141,513 - hpt b0125 0.15 +0.0 141,566 + hpt b0125 0.25 -0.2 141,566 + hpt b0125 0.25 +0.0 141,574 - hpt b0125 0.27 -1.4 141,574 - hpt b0125 0.27 -1.7 141,586 + hpt b0125 0.29 -0.6 141,588 + hpt b0125 0.29 -2.0 141,588 + hpt b0125 0.29 -0.4 141,591 - hpt b0125 0.30 -1.2 141,591 - hpt b0125 0.30 -0.7 141,618 + hpt b0125 0.35 -2.0 141,899 + hpt b0125 0.87 -1.9 141,906 + hpt b0125 0.88 +0.9 141,910 + hpt b0125 0.89 -0.4 141,910 + hpt b0125 0.89 -0.1 141,967 + +1.1 142,000 - -0.3 142,000 - +0.0 142,836 - -0.6 142,836 - -0.2 142,925 - yadG b0127 0.16 -0.1
Or see this region's nucleotide sequence